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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Salmonella typhimurium Aromatic amino acid transport protein AroP (aroP), partial CSB-YP315644SXB
CSB-EP315644SXB
CSB-BP315644SXB
CSB-MP315644SXB
CSB-EP315644SXB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella typhimurium Glutamate-1-semialdehyde 2,1-aminomutase (hemL) CSB-YP315645SXB
CSB-EP315645SXB
CSB-BP315645SXB
CSB-MP315645SXB
CSB-EP315645SXB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella typhimurium Pyruvate dehydrogenase complex repressor (pdhR) CSB-YP315646SXB
CSB-EP315646SXB
CSB-BP315646SXB
CSB-MP315646SXB
CSB-EP315646SXB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella typhimurium Invasion protein iagB (iagB) CSB-YP315647SXB
CSB-BP315647SXB
CSB-MP315647SXB
CSB-EP315647SXB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Coccidioides immitis Ornithine carbamoyltransferase, mitochondrial (CIMG_04084), partial CSB-YP315648DUV
CSB-EP315648DUV
CSB-BP315648DUV
CSB-MP315648DUV
CSB-EP315648DUV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Putative uncharacterized protein YER190C-A (YER190C-A), partial CSB-YP315649SVG1
CSB-EP315649SVG1
CSB-BP315649SVG1
CSB-MP315649SVG1
CSB-EP315649SVG1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Putative uncharacterized protein YNL339W-A (YNL339W-A), partial CSB-YP315650SVG1
CSB-EP315650SVG1
CSB-BP315650SVG1
CSB-MP315650SVG1
CSB-EP315650SVG1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Putative UPF0479 protein YLR466C-A (YLR466C-A), partial CSB-YP315651SVG1
CSB-EP315651SVG1
CSB-BP315651SVG1
CSB-MP315651SVG1
CSB-EP315651SVG1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Putative UPF0479 protein YIL177W-A (YIL177W-A), partial CSB-YP315652SVG1
CSB-EP315652SVG1
CSB-BP315652SVG1
CSB-MP315652SVG1
CSB-EP315652SVG1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella typhimurium Oxygen-regulated invasion protein OrgA (orgA) CSB-YP315653SXB
CSB-EP315653SXB
CSB-BP315653SXB
CSB-MP315653SXB
CSB-EP315653SXB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella typhimurium Arginine--tRNA ligase (argS), partial CSB-YP315654SXB
CSB-EP315654SXB
CSB-BP315654SXB
CSB-MP315654SXB
CSB-EP315654SXB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Borrelia burgdorferi Protein BptA (bptA) CSB-YP315655BUD
CSB-EP315655BUD
CSB-BP315655BUD
CSB-MP315655BUD
CSB-EP315655BUD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Borrelia burgdorferi Decorin-binding protein B (dbpB) CSB-YP315656BUD
CSB-EP315656BUD
CSB-BP315656BUD
CSB-MP315656BUD
CSB-EP315656BUD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Glutamate dehydrogenase (gdhA) CSB-YP315657PXS
CSB-EP315657PXS
CSB-BP315657PXS
CSB-MP315657PXS
CSB-EP315657PXS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pyrococcus abyssi Orotate phosphoribosyltransferase (pyrE) CSB-YP315658FHV
CSB-EP315658FHV
CSB-BP315658FHV
CSB-MP315658FHV
CSB-EP315658FHV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cryptococcus neoformans var. neoformans serotype D Probable acetate kinase (CNN01560) CSB-YP315660CTL
CSB-EP315660CTL
CSB-BP315660CTL
CSB-MP315660CTL
CSB-EP315660CTL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cryptococcus neoformans var. neoformans serotype D Actin-like protein ARP6 (ARP6) CSB-YP315661CTL
CSB-EP315661CTL
CSB-BP315661CTL
CSB-MP315661CTL
CSB-EP315661CTL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cryptococcus neoformans var. neoformans serotype D Actin-like protein ARP6 (ARP6) CSB-YP315662CTK
CSB-EP315662CTK
CSB-BP315662CTK
CSB-MP315662CTK
CSB-EP315662CTK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cryptococcus neoformans var. neoformans serotype D Fe-S cluster assembly protein DRE2 (DRE2) CSB-YP315663CTK
CSB-EP315663CTK
CSB-BP315663CTK
CSB-MP315663CTK
CSB-EP315663CTK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cryptococcus neoformans var. neoformans serotype D ADP-ribosylation factor (ARF) CSB-YP315664CTK
CSB-EP315664CTK
CSB-BP315664CTK
CSB-MP315664CTK
CSB-EP315664CTK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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