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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Escherichia coli Transposase InsI for insertion sequence element IS30D (insI4) CSB-YP317179ENV
CSB-EP317179ENV
CSB-BP317179ENV
CSB-MP317179ENV
CSB-EP317179ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse C2 calcium-dependent domain-containing protein 4D (C2cd4d) CSB-YP317180MO
CSB-EP317180MO
CSB-BP317180MO
CSB-MP317180MO
CSB-EP317180MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Zinc finger protein 883 (ZNF883) CSB-YP317181HU
CSB-EP317181HU
CSB-BP317181HU
CSB-MP317181HU
CSB-EP317181HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Gorilla gorilla gorilla Polyubiquitin-C (UBC) CSB-YP317182GGZ
CSB-EP317182GGZ
CSB-BP317182GGZ
CSB-MP317182GGZ
CSB-EP317182GGZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Schizosaccharomyces pombe Polyubiquitin (ubi4) CSB-YP317183SXV
CSB-EP317183SXV
CSB-BP317183SXV
CSB-MP317183SXV
CSB-EP317183SXV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Hordeum vulgare Polyubiquitin CSB-YP317184HWQ
CSB-EP317184HWQ
CSB-BP317184HWQ
CSB-MP317184HWQ
CSB-EP317184HWQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arthrobacter aurescens Prokaryotic ubiquitin-like protein Pup (pup) CSB-YP317185AUZ
CSB-EP317185AUZ
CSB-BP317185AUZ
CSB-MP317185AUZ
CSB-EP317185AUZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium smegmatis Ribonucleoside-diphosphate reductase subunit alpha 2 (nrdE2), partial CSB-YP317186MVX
CSB-EP317186MVX
CSB-BP317186MVX
CSB-MP317186MVX
CSB-EP317186MVX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Petroselinum crispum Polyubiquitin (PCUBI4-1) CSB-YP317187PGL
CSB-EP317187PGL
CSB-BP317187PGL
CSB-MP317187PGL
CSB-EP317187PGL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Conus parius Conotoxin pr6a CSB-YP317188COZ
CSB-EP317188COZ
CSB-BP317188COZ
CSB-MP317188COZ
CSB-EP317188COZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Conus pulicarius Conotoxin Pu3.6 CSB-YP317189DWG
CSB-EP317189DWG
CSB-BP317189DWG
CSB-MP317189DWG
CSB-EP317189DWG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vespa magnifica Probable phospholipase A1 magnifin CSB-YP317190VBQ
CSB-EP317190VBQ
CSB-BP317190VBQ
CSB-MP317190VBQ
CSB-EP317190VBQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chilobrachys jingzhao U17-theraphotoxin-Cj1a CSB-YP317191DRU
CSB-EP317191DRU
CSB-BP317191DRU
CSB-MP317191DRU
CSB-EP317191DRU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ustilago maydis Putative uncharacterized protein UM10309 (UM10309), partial CSB-YP317192UBS
CSB-EP317192UBS
CSB-BP317192UBS
CSB-MP317192UBS
CSB-EP317192UBS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Haplopelma hainanum Hainantoxin F4-23.43 CSB-YP317193HUK
CSB-EP317193HUK
CSB-BP317193HUK
CSB-MP317193HUK
CSB-EP317193HUK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Haplopelma hainanum Hainantoxin F8-35.23 CSB-YP317194HUK
CSB-EP317194HUK
CSB-BP317194HUK
CSB-MP317194HUK
CSB-EP317194HUK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nematostella vectensis Neurotoxin Nv1-116.37.1 (v1g113210) CSB-YP317195NGO
CSB-EP317195NGO
CSB-BP317195NGO
CSB-MP317195NGO
CSB-EP317195NGO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Coccidioides immitis Deoxyribose-phosphate aldolase (CIMG_00461) CSB-YP317196DUV
CSB-EP317196DUV
CSB-BP317196DUV
CSB-MP317196DUV
CSB-EP317196DUV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Putative protein FAM106C (FAM106CP) CSB-YP317197HU
CSB-EP317197HU
CSB-BP317197HU
CSB-MP317197HU
CSB-EP317197HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aphonopelma californicum Omega-theraphotoxin-Asp3a CSB-YP317198AMF
CSB-EP317198AMF
CSB-BP317198AMF
CSB-MP317198AMF
CSB-EP317198AMF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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