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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Bean golden yellow mosaic virus Transcriptional activator protein (AC2, AL2) CSB-YP317239BFS
CSB-EP317239BFS
CSB-BP317239BFS
CSB-MP317239BFS
CSB-EP317239BFS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bean golden yellow mosaic virus Movement protein BC1 (BC1, BL1) CSB-YP317240BFS
CSB-EP317240BFS
CSB-BP317240BFS
CSB-MP317240BFS
CSB-EP317240BFS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Epstein-Barr virus Tegument protein BGLF2 (BGLF2) CSB-YP317241EFC
CSB-EP317241EFC
CSB-BP317241EFC
CSB-MP317241EFC
CSB-EP317241EFC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Faba bean necrotic yellows virus Para-Rep C9 (C9) CSB-YP317242FDZ
CSB-EP317242FDZ
CSB-BP317242FDZ
CSB-MP317242FDZ
CSB-EP317242FDZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Putative lipoprotein AcfD homolog (yghJ), partial CSB-YP317243ENV
CSB-EP317243ENV
CSB-BP317243ENV
CSB-MP317243ENV
CSB-EP317243ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella typhimurium Protein CsiD (csiD) CSB-YP317244SXB
CSB-EP317244SXB
CSB-BP317244SXB
CSB-MP317244SXB
CSB-EP317244SXB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella typhimurium Inner membrane transporter rhtA (rhtA), partial CSB-YP317245SXB
CSB-EP317245SXB
CSB-BP317245SXB
CSB-MP317245SXB
CSB-EP317245SXB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella typhimurium HTH-type transcriptional regulator galR (galR) CSB-YP317246SXB
CSB-EP317246SXB
CSB-BP317246SXB
CSB-MP317246SXB
CSB-EP317246SXB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella typhimurium Transcriptional regulatory protein tctD (tctD) CSB-YP317247SXB
CSB-EP317247SXB
CSB-BP317247SXB
CSB-MP317247SXB
CSB-EP317247SXB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Putative UPF0377 protein YIL174W (YIL174W) CSB-YP317248SVG
CSB-EP317248SVG
CSB-BP317248SVG
CSB-MP317248SVG
CSB-EP317248SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Putative uncharacterized protein YGR296C-A (YGR296C-A), partial CSB-YP317249SVG1
CSB-EP317249SVG1
CSB-BP317249SVG1
CSB-MP317249SVG1
CSB-EP317249SVG1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Putative uncharacterized protein YPL283W-A (YPL283W-A), partial CSB-YP317250SVG1
CSB-EP317250SVG1
CSB-BP317250SVG1
CSB-MP317250SVG1
CSB-EP317250SVG1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Putative UPF0479 protein YHR219C-A (YHR219C-A), partial CSB-YP317251SVG1
CSB-EP317251SVG1
CSB-BP317251SVG1
CSB-MP317251SVG1
CSB-EP317251SVG1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Putative UPF0479 protein YDR545C-A (YDR545C-A), partial CSB-YP317252SVG1
CSB-EP317252SVG1
CSB-BP317252SVG1
CSB-MP317252SVG1
CSB-EP317252SVG1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella typhimurium SPI-1 type 3 secretion system pilotin (invH) CSB-YP317253SXB
CSB-EP317253SXB
CSB-BP317253SXB
CSB-MP317253SXB
CSB-EP317253SXB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella typhimurium Cell invasion protein SipC (sipC) CSB-YP317254SXB
CSB-EP317254SXB
CSB-BP317254SXB
CSB-MP317254SXB
CSB-EP317254SXB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella typhimurium Cell invasion protein SipA (sipA), partial CSB-YP317255SXB
CSB-EP317255SXB
CSB-BP317255SXB
CSB-MP317255SXB
CSB-EP317255SXB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Antitoxin MazE6 (mazE6) CSB-YP317256MVZ
CSB-EP317256MVZ
CSB-BP317256MVZ
CSB-MP317256MVZ
CSB-EP317256MVZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Borrelia burgdorferi Outer surface protein A (ospA) CSB-YP317257BUD
CSB-EP317257BUD
CSB-BP317257BUD
CSB-MP317257BUD
CSB-EP317257BUD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pyrococcus abyssi DNA polymerase 1 (polI), partial CSB-YP317258FHV
CSB-EP317258FHV
CSB-BP317258FHV
CSB-MP317258FHV
CSB-EP317258FHV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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