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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Chicken Non-histone chromosomal protein HMG-14A CSB-YP319023CH
CSB-EP319023CH
CSB-BP319023CH
CSB-MP319023CH
CSB-EP319023CH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Murine polyomavirus Middle T antigen, partial CSB-YP319024MKJ
CSB-EP319024MKJ
CSB-BP319024MKJ
CSB-MP319024MKJ
CSB-EP319024MKJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Murine polyomavirus Major capsid protein VP1 CSB-YP319025MKJ
CSB-EP319025MKJ
CSB-BP319025MKJ
CSB-MP319025MKJ
CSB-EP319025MKJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chimpanzee hepatitis B virus Protein X (X) CSB-YP319026CIJ
CSB-EP319026CIJ
CSB-BP319026CIJ
CSB-MP319026CIJ
CSB-EP319026CIJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bovine enterovirus Genome polyprotein CSB-YP319027BJP
CSB-EP319027BJP
CSB-BP319027BJP
CSB-MP319027BJP
CSB-EP319027BJP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae N-terminal acetyltransferase A complex subunit NAT1 (NAT1), partial CSB-YP319028SVG
CSB-EP319028SVG
CSB-BP319028SVG
CSB-MP319028SVG
CSB-EP319028SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Hordeum vulgare Dehydrin DHN4 (DHN4) CSB-YP319029HWQ
CSB-EP319029HWQ
CSB-BP319029HWQ
CSB-MP319029HWQ
CSB-EP319029HWQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Zea mays Regulatory protein opaque-2 (O2) CSB-YP319030ZAX
CSB-EP319030ZAX
CSB-BP319030ZAX
CSB-MP319030ZAX
CSB-EP319030ZAX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lysinibacillus sphaericus 41.9 kDa insecticidal toxin (binA) CSB-YP319031LRU
CSB-EP319031LRU
CSB-BP319031LRU
CSB-MP319031LRU
CSB-EP319031LRU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Epstein-Barr virus Epstein-Barr nuclear antigen 3 (EBNA3), partial CSB-YP319032EFA
CSB-BP319032EFA
CSB-MP319032EFA
CSB-EP319032EFA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant ATP synthase protein I (atpI), partial CSB-YP319033VEU1
CSB-EP319033VEU1
CSB-BP319033VEU1
CSB-MP319033VEU1
CSB-EP319033VEU1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Adenosylmethionine-8-amino-7-oxononanoate aminotransferase (bioA) CSB-YP319034ENV
CSB-BP319034ENV
CSB-MP319034ENV
CSB-EP319034ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Pimeloyl-[acyl-carrier protein] methyl ester esterase (bioH) CSB-YP319035ENV
CSB-BP319035ENV
CSB-MP319035ENV
CSB-EP319035ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Protein FdhE (fdhE) CSB-YP319036ENV
CSB-EP319036ENV
CSB-BP319036ENV
CSB-MP319036ENV
CSB-EP319036ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Catalase-peroxidase (katG) CSB-YP319037ENV
CSB-EP319037ENV
CSB-BP319037ENV
CSB-MP319037ENV
CSB-EP319037ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Enterochelin esterase (fes) CSB-YP319038ENV
CSB-EP319038ENV
CSB-BP319038ENV
CSB-MP319038ENV
CSB-EP319038ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Halobacterium salinarum Protein gvpD 1 (gvpD11), partial CSB-YP319039HTM
CSB-EP319039HTM
CSB-BP319039HTM
CSB-MP319039HTM
CSB-EP319039HTM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Enterobacteria phage P4 Protein beta (Beta) CSB-YP319040EDH
CSB-EP319040EDH
CSB-BP319040EDH
CSB-MP319040EDH
CSB-EP319040EDH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Periplasmic [NiFe] hydrogenase small subunit (hydA) CSB-YP319041DDE
CSB-EP319041DDE
CSB-BP319041DDE
CSB-MP319041DDE
CSB-EP319041DDE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Desulfomicrobium norvegicum Periplasmic [NiFeSe] hydrogenase large subunit CSB-YP319042DCZ
CSB-EP319042DCZ
CSB-BP319042DCZ
CSB-MP319042DCZ
CSB-EP319042DCZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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