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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Nicotiana sylvestris 33 kDa ribonucleoprotein, chloroplastic CSB-YP323138NHD
CSB-EP323138NHD
CSB-BP323138NHD
CSB-MP323138NHD
CSB-EP323138NHD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Infectious hematopoietic necrosis virus Nucleoprotein (N) CSB-YP323139IAP
CSB-EP323139IAP
CSB-BP323139IAP
CSB-MP323139IAP
CSB-EP323139IAP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Influenza A virus Hemagglutinin (HA), partial CSB-YP323140ILL
CSB-EP323140ILL
CSB-BP323140ILL
CSB-MP323140ILL
CSB-EP323140ILL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Influenza A virus Hemagglutinin (HA), partial CSB-YP323141IIA
CSB-EP323141IIA
CSB-BP323141IIA
CSB-MP323141IIA
CSB-EP323141IIA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oncorhynchus keta Pro-MCH 1 (mch1) CSB-YP323142OBG
CSB-EP323142OBG
CSB-BP323142OBG
CSB-MP323142OBG
CSB-EP323142OBG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mumps virus Matrix protein (M) CSB-YP323143MJM
CSB-EP323143MJM
CSB-BP323143MJM
CSB-MP323143MJM
CSB-EP323143MJM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Enterobacteria phage T7 Major capsid protein (10) CSB-YP323144EEB
CSB-EP323144EEB
CSB-BP323144EEB
CSB-MP323144EEB
CSB-EP323144EEB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas sp. Phenol hydroxylase P0 protein (dmpK) CSB-YP323145PVK
CSB-EP323145PVK
CSB-BP323145PVK
CSB-MP323145PVK
CSB-EP323145PVK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Murine coronavirus Non-structural protein 2a (2a) CSB-YP323146MJY
CSB-EP323146MJY
CSB-BP323146MJY
CSB-MP323146MJY
CSB-EP323146MJY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Feline coronavirus Non-structural protein 7b (7b) CSB-YP323147FEJ
CSB-EP323147FEJ
CSB-BP323147FEJ
CSB-MP323147FEJ
CSB-EP323147FEJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neurospora crassa 30 kDa heat shock protein (hsp30) CSB-YP323148NHA
CSB-EP323148NHA
CSB-BP323148NHA
CSB-MP323148NHA
CSB-EP323148NHA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mumps virus Hemagglutinin-neuraminidase (HN), partial CSB-YP323149MJM2
CSB-EP323149MJM2
CSB-BP323149MJM2
CSB-MP323149MJM2
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Insertion element IS150 uncharacterized 19.7 kDa protein (insJ) CSB-YP323150ENV
CSB-EP323150ENV
CSB-BP323150ENV
CSB-MP323150ENV
CSB-EP323150ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptomyces coelicolor Insertion element IS110 uncharacterized 43.6 kDa protein (SCO1005) CSB-YP323151FOB
CSB-EP323151FOB
CSB-BP323151FOB
CSB-MP323151FOB
CSB-EP323151FOB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 3-phosphoshikimate 1-carboxyvinyltransferase (aroA) CSB-YP323152SWW
CSB-EP323152SWW
CSB-BP323152SWW
CSB-MP323152SWW
CSB-EP323152SWW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aspergillus saitoi Ribonuclease M CSB-YP323153APR
CSB-EP323153APR
CSB-BP323153APR
CSB-MP323153APR
CSB-EP323153APR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Endopolygalacturonase II (pgaII) CSB-YP323154DPD
CSB-EP323154DPD
CSB-BP323154DPD
CSB-MP323154DPD
CSB-EP323154DPD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Equine arteritis virus Nucleoprotein (N) CSB-YP323155EFH
CSB-EP323155EFH
CSB-BP323155EFH
CSB-MP323155EFH
CSB-EP323155EFH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Trigonoceps occipitalis Hemoglobin subunit alpha-A (HBAA) CSB-YP323157TII
CSB-EP323157TII
CSB-BP323157TII
CSB-MP323157TII
CSB-EP323157TII-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas stutzeri Membrane protein nosY, partial CSB-YP323158FGJ1
CSB-EP323158FGJ1
CSB-BP323158FGJ1
CSB-MP323158FGJ1
CSB-EP323158FGJ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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