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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Streptomyces coelicolor Probable glycerol uptake facilitator protein (glpF), partial CSB-YP324786FOB1
CSB-EP324786FOB1
CSB-BP324786FOB1
CSB-MP324786FOB1
CSB-EP324786FOB1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella typhimurium Ethanolamine ammonia-lyase light chain (eutC) CSB-YP324787SXB
CSB-EP324787SXB
CSB-BP324787SXB
CSB-MP324787SXB
CSB-EP324787SXB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermoproteus tenax virus 1 Coat protein TP2 CSB-YP324788TDO
CSB-EP324788TDO
CSB-BP324788TDO
CSB-MP324788TDO
CSB-EP324788TDO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermoproteus tenax virus 1 Viral protein TPX CSB-YP324789TDO
CSB-EP324789TDO
CSB-BP324789TDO
CSB-MP324789TDO
CSB-EP324789TDO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermoproteus tenax virus 1 Uncharacterized 9.5 kDa protein CSB-YP324790TDO
CSB-EP324790TDO
CSB-BP324790TDO
CSB-MP324790TDO
CSB-EP324790TDO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermoproteus tenax virus 1 Uncharacterized 9.4 kDa protein CSB-YP324791TDO
CSB-EP324791TDO
CSB-BP324791TDO
CSB-MP324791TDO
CSB-EP324791TDO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermoproteus tenax virus 1 Uncharacterized 16.6 kDa protein CSB-YP324792TDO
CSB-EP324792TDO
CSB-BP324792TDO
CSB-MP324792TDO
CSB-EP324792TDO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermoproteus tenax virus 1 Uncharacterized 38.6 kDa protein CSB-YP324793TDO
CSB-EP324793TDO
CSB-BP324793TDO
CSB-MP324793TDO
CSB-EP324793TDO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermoproteus tenax virus 1 Uncharacterized 8.8 kDa protein CSB-YP324794TDO
CSB-EP324794TDO
CSB-BP324794TDO
CSB-MP324794TDO
CSB-EP324794TDO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lemna gibba Ribulose bisphosphate carboxylase small chain SSU40A, chloroplastic (SSU40A) CSB-YP324795LDU
CSB-EP324795LDU
CSB-BP324795LDU
CSB-MP324795LDU
CSB-EP324795LDU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Respiratory nitrate reductase 2 alpha chain (narZ), partial CSB-YP324796ENV
CSB-EP324796ENV
CSB-BP324796ENV
CSB-MP324796ENV
CSB-EP324796ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lymphocytic choriomeningitis virus RING finger protein Z (Z) CSB-YP324797LKY
CSB-EP324797LKY
CSB-BP324797LKY
CSB-MP324797LKY
CSB-EP324797LKY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Phaseolus vulgaris Arcelin-2 (ARC2) CSB-YP324798EUN
CSB-EP324798EUN
CSB-BP324798EUN
CSB-MP324798EUN
CSB-EP324798EUN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Enterobacteria phage BF23 Probable protein A1 (A1) CSB-YP324799ECE
CSB-EP324799ECE
CSB-BP324799ECE
CSB-MP324799ECE
CSB-EP324799ECE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Artemia sp. Extracellular globin-E1 CSB-YP324800AOF
CSB-EP324800AOF
CSB-BP324800AOF
CSB-MP324800AOF
CSB-EP324800AOF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Flexible pilin (aerA) CSB-YP324801AXW
CSB-EP324801AXW
CSB-BP324801AXW
CSB-MP324801AXW
CSB-EP324801AXW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Strongylocentrotus purpuratus Histone H1, early embryonic CSB-YP324802FOT
CSB-EP324802FOT
CSB-BP324802FOT
CSB-MP324802FOT
CSB-EP324802FOT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nicotiana tabacum 60S ribosomal protein L40 CSB-YP324803NHE
CSB-EP324803NHE
CSB-BP324803NHE
CSB-MP324803NHE
CSB-EP324803NHE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus phage Cp-9 Lysozyme (CPL9) CSB-YP324804SMO
CSB-EP324804SMO
CSB-BP324804SMO
CSB-MP324804SMO
CSB-EP324804SMO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Eubacterium sp. Bile acid 7-alpha dehydratase (baiE) CSB-YP324805EHF
CSB-EP324805EHF
CSB-BP324805EHF
CSB-MP324805EHF
CSB-EP324805EHF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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