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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Mesobuthus eupeus Toxin BeI2 CSB-YP323346MRD
CSB-EP323346MRD
CSB-BP323346MRD
CSB-MP323346MRD
CSB-EP323346MRD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Tityus serrulatus Beta-mammal/insect toxin Ts1 CSB-YP323347TON
CSB-MP323347TON
CSB-EP323347TON-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Enterobacteria phage N4 Uncharacterized protein Gp1 CSB-YP323348EDB
CSB-EP323348EDB
CSB-BP323348EDB
CSB-MP323348EDB
CSB-EP323348EDB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sheep Keratin, type II microfibrillar, component 7C CSB-YP323349SH
CSB-EP323349SH
CSB-BP323349SH
CSB-MP323349SH
CSB-EP323349SH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chromosomal protein MC1a CSB-YP323350MFH
CSB-EP323350MFH
CSB-BP323350MFH
CSB-MP323350MFH
CSB-EP323350MFH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Zea mays Autonomous transposable element EN-1 mosaic protein, partial CSB-YP323351ZAX
CSB-EP323351ZAX
CSB-BP323351ZAX
CSB-MP323351ZAX
CSB-EP323351ZAX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sinapis alba Allergen Sin a 1 CSB-YP323352SZO
CSB-EP323352SZO
CSB-BP323352SZO
CSB-MP323352SZO
CSB-EP323352SZO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nitrogenase vanadium-iron protein alpha chain (vnfD) CSB-YP323353DPU
CSB-EP323353DPU
CSB-BP323353DPU
CSB-MP323353DPU
CSB-EP323353DPU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycoplasma hyorhinis High affinity transport system protein p37 (p37) CSB-YP323355MLR
CSB-BP323355MLR
CSB-MP323355MLR
CSB-EP323355MLR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rat Probasin (Pbsn) CSB-YP323356RA
CSB-EP323356RA
CSB-BP323356RA
CSB-MP323356RA
CSB-EP323356RA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae ATP-dependent RNA helicase MSS116, mitochondrial (MSS116), partial CSB-YP323357SVG
CSB-EP323357SVG
CSB-BP323357SVG
CSB-MP323357SVG
CSB-EP323357SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Struthio camelus Neurophysin 1 CSB-YP323358FOU
CSB-EP323358FOU
CSB-BP323358FOU
CSB-MP323358FOU
CSB-EP323358FOU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Apis mellifera Abaecin CSB-YP323359DNK
CSB-EP323359DNK
CSB-BP323359DNK
CSB-MP323359DNK
CSB-EP323359DNK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana 2S seed storage protein 1 (AT2S1) CSB-YP323360DOA
CSB-EP323360DOA
CSB-BP323360DOA
CSB-MP323360DOA
CSB-EP323360DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Solanum tuberosum Patatin-B1 (PATB1) CSB-YP323361FIG
CSB-EP323361FIG
CSB-BP323361FIG
CSB-MP323361FIG
CSB-EP323361FIG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli CS3 fimbrial subunit A CSB-YP323362ENL
CSB-EP323362ENL
CSB-BP323362ENL
CSB-MP323362ENL
CSB-EP323362ENL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dictyostelium discoideum Spore germination protein 3 (gerC) CSB-YP323363DKK
CSB-EP323363DKK
CSB-BP323363DKK
CSB-MP323363DKK
CSB-EP323363DKK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prosthecochloris aestuarii Bacteriochlorophyll c-binding protein (csmA), partial CSB-YP323364EYX
CSB-EP323364EYX
CSB-BP323364EYX
CSB-MP323364EYX
CSB-EP323364EYX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Podospora anserina Uncharacterized 50.9 kDa protein in ND4 intron 1 CSB-YP323365EXJ
CSB-EP323365EXJ
CSB-BP323365EXJ
CSB-MP323365EXJ
CSB-EP323365EXJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Agrobacterium tumefaciens Conjugal transfer protein traF (traF) CSB-YP323366AEZ
CSB-EP323366AEZ
CSB-BP323366AEZ
CSB-MP323366AEZ
CSB-EP323366AEZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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