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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Triticum aestivum Alpha-amylase/trypsin inhibitor CM16 CSB-YP325407TQN
CSB-EP325407TQN
CSB-BP325407TQN
CSB-MP325407TQN
CSB-EP325407TQN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mercuric reductase (merA), partial CSB-YP325408BQJ
CSB-EP325408BQJ
CSB-BP325408BQJ
CSB-MP325408BQJ
CSB-EP325408BQJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human HLA class I histocompatibility antigen, A-30 alpha chain (HLA-A), partial CSB-YP325409HU
CSB-EP325409HU
CSB-BP325409HU
CSB-MP325409HU
CSB-EP325409HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Influenza B virus Glycoprotein NB (NB), partial CSB-YP325410IJN1
CSB-EP325410IJN1
CSB-BP325410IJN1
CSB-MP325410IJN1
CSB-EP325410IJN1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Influenza B virus Glycoprotein NB (NB), partial CSB-YP325411IJV1
CSB-EP325411IJV1
CSB-BP325411IJV1
CSB-MP325411IJV1
CSB-EP325411IJV1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pongo pygmaeus Popy class I histocompatibility antigen, alpha chain E (Popy-E), partial CSB-YP325412EXP
CSB-EP325412EXP
CSB-BP325412EXP
CSB-MP325412EXP
CSB-EP325412EXP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Locusta migratoria Locustatachykinin-1 CSB-YP325413LHY
CSB-EP325413LHY
CSB-BP325413LHY
CSB-MP325413LHY
CSB-EP325413LHY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Sodium/pantothenate symporter (panF), partial CSB-YP325414ENV
CSB-EP325414ENV
CSB-BP325414ENV
CSB-MP325414ENV
CSB-EP325414ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Hordeum vulgare Pathogen-related protein CSB-YP325415HWQ
CSB-EP325415HWQ
CSB-BP325415HWQ
CSB-MP325415HWQ
CSB-EP325415HWQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rabies virus Glycoprotein (G), partial CSB-YP325416RAJ1
CSB-EP325416RAJ1
CSB-BP325416RAJ1
CSB-MP325416RAJ1
CSB-EP325416RAJ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Amaranthus hypochondriacus Ribulose bisphosphate carboxylase large chain (rbcL) CSB-YP325417BYA
CSB-EP325417BYA
CSB-BP325417BYA
CSB-MP325417BYA
CSB-EP325417BYA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Serralysin C (prtC) CSB-YP325418EGI
CSB-EP325418EGI
CSB-BP325418EGI
CSB-MP325418EGI
CSB-EP325418EGI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Medicago sativa Bowman-Birk type wound-induced trypsin inhibitor CSB-YP325419MQO
CSB-EP325419MQO
CSB-BP325419MQO
CSB-MP325419MQO
CSB-EP325419MQO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Crotalaria juncea Lectin CSB-YP325420CSP
CSB-EP325420CSP
CSB-BP325420CSP
CSB-MP325420CSP
CSB-EP325420CSP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila pseudoobscura pseudoobscura Pupal cuticle protein (Pcp) CSB-YP325421DME
CSB-EP325421DME
CSB-BP325421DME
CSB-MP325421DME
CSB-EP325421DME-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila melanogaster Guanine nucleotide-binding protein G (o) subunit alpha 47A CSB-YP325422DLU
CSB-EP325422DLU
CSB-BP325422DLU
CSB-MP325422DLU
CSB-EP325422DLU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Pyruvate dehydrogenase E1 component subunit alpha, mitochondrial (PDA1) CSB-YP325423SVG
CSB-EP325423SVG
CSB-BP325423SVG
CSB-MP325423SVG
CSB-EP325423SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Minor extracellular protease epr (epr), partial CSB-YP325424BRJ
CSB-EP325424BRJ
CSB-BP325424BRJ
CSB-MP325424BRJ
CSB-EP325424BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Formate hydrogenlyase subunit 7 (hycG) CSB-YP325425ENV
CSB-EP325425ENV
CSB-BP325425ENV
CSB-MP325425ENV
CSB-EP325425ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae 78 kDa glucose-regulated protein homolog (KAR2), partial CSB-YP325426SVG
CSB-EP325426SVG
CSB-BP325426SVG
CSB-MP325426SVG
CSB-EP325426SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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