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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Escherichia coli Protein rhsD (rhsD), partial CSB-YP325467ENV
CSB-EP325467ENV
CSB-BP325467ENV
CSB-MP325467ENV
CSB-EP325467ENV-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia cepacia HTH-type transcriptional regulator dgdR (dgdR) CSB-YP325468BXR
CSB-EP325468BXR
CSB-BP325468BXR
CSB-MP325468BXR
CSB-EP325468BXR-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Insertion element IS600 uncharacterized 31 kDa protein CSB-YP325469SGH
CSB-EP325469SGH
CSB-BP325469SGH
CSB-MP325469SGH
CSB-EP325469SGH-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Influenza A virus Nucleoprotein (NP) CSB-YP325470IBK
CSB-EP325470IBK
CSB-BP325470IBK
CSB-MP325470IBK
CSB-EP325470IBK-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Influenza A virus Hemagglutinin (HA), partial CSB-YP325471IDN
CSB-EP325471IDN
CSB-BP325471IDN
CSB-MP325471IDN
CSB-EP325471IDN-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Influenza A virus Hemagglutinin (HA), partial CSB-YP325472IEA
CSB-EP325472IEA
CSB-BP325472IEA
CSB-MP325472IEA
CSB-EP325472IEA-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Zinc finger protein 28 (ZNF28) CSB-YP325473HU
CSB-EP325473HU
CSB-BP325473HU
CSB-MP325473HU
CSB-EP325473HU-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant HTH-type transcriptional regulator AlkS (alkS), partial CSB-YP325474PUQ
CSB-EP325474PUQ
CSB-BP325474PUQ
CSB-MP325474PUQ
CSB-EP325474PUQ-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodobacter capsulatus Methoxyneurosporene dehydrogenase (crtD) CSB-YP325475RLD
CSB-EP325475RLD
CSB-BP325475RLD
CSB-MP325475RLD
CSB-EP325475RLD-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Rab guanine nucleotide exchange factor SEC2 (SEC2), partial CSB-YP325476SVG
CSB-EP325476SVG
CSB-BP325476SVG
CSB-MP325476SVG
CSB-EP325476SVG-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Proteus mirabilis Urease accessory protein UreD (ureD) CSB-YP325477EYZ
CSB-EP325477EYZ
CSB-BP325477EYZ
CSB-MP325477EYZ
CSB-EP325477EYZ-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sulfolobus acidocaldarius Thermopsin (thpS) CSB-YP325478FOZ
CSB-EP325478FOZ
CSB-BP325478FOZ
CSB-MP325478FOZ
CSB-EP325478FOZ-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Hydra vulgaris Actin, non-muscle 6.2 CSB-YP325479HXN
CSB-EP325479HXN
CSB-BP325479HXN
CSB-MP325479HXN
CSB-EP325479HXN-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Endo-1,4-beta-xylanase (xynB) CSB-YP325480CME
CSB-EP325480CME
CSB-BP325480CME
CSB-MP325480CME
CSB-EP325480CME-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human cytomegalovirus Uncharacterized protein US25 (US25) CSB-YP325481HWV
CSB-EP325481HWV
CSB-BP325481HWV
CSB-MP325481HWV
CSB-EP325481HWV-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ribosome hibernation promoting factor (hpf) CSB-YP325482KBE
CSB-EP325482KBE
CSB-BP325482KBE
CSB-MP325482KBE
CSB-EP325482KBE-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Enterobacteria phage T4 Baseplate structural protein Gp27 (27) CSB-YP325483EDZ
CSB-EP325483EDZ
CSB-BP325483EDZ
CSB-MP325483EDZ
CSB-EP325483EDZ-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sulfolobus acidocaldarius Elongation factor 1-alpha (tuf) CSB-YP325484FOZ
CSB-EP325484FOZ
CSB-BP325484FOZ
CSB-MP325484FOZ
CSB-EP325484FOZ-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila melanogaster Serine protease 3 (Jon99Ci) CSB-YP325485DLU
CSB-EP325485DLU
CSB-BP325485DLU
CSB-MP325485DLU
CSB-EP325485DLU-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Telomere elongation protein EST1 (EST1), partial CSB-YP325486SVG
CSB-EP325486SVG
CSB-BP325486SVG
CSB-MP325486SVG
CSB-EP325486SVG-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式，可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中，除了表達(dá)系統(tǒng)之外，還面臨著一系列令人困惑的選擇，如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí)，你必須做出很多決定。如果你選擇的明智，你將獲得高質(zhì)量的重組蛋白，后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定，你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里，來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示，可以幫助你做出明智的決策。

第一條：重組蛋白的生產(chǎn)，在這篇文章中，我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條：如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子，它攜帶特定的基因進(jìn)入宿主細(xì)胞，并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá)，經(jīng)過多年的發(fā)展，表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示，本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條：如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用，對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題，并提供一些解決方案。除了以上文章，我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外，我們還為我們的客戶提供重組蛋白表達(dá)服務(wù)，您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問，涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等，您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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