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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Influenza A virus Non-structural protein 1 (NS) CSB-YP325975IGX
CSB-EP325975IGX
CSB-BP325975IGX
CSB-MP325975IGX
CSB-EP325975IGX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Invertebrate iridescent virus 6 Uncharacterized protein 443R (IIV6-443R), partial CSB-YP325976INA
CSB-EP325976INA
CSB-BP325976INA
CSB-MP325976INA
CSB-EP325976INA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Enterobacter aerogenes Urease subunit gamma (ureA) CSB-YP325977EKN
CSB-EP325977EKN
CSB-BP325977EKN
CSB-MP325977EKN
CSB-EP325977EKN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clypeaster japonicus Profilin CSB-YP325978CMU
CSB-EP325978CMU
CSB-BP325978CMU
CSB-MP325978CMU
CSB-EP325978CMU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Transposon Tn552 resolvase (tnpR) CSB-YP325979FKZ
CSB-EP325979FKZ
CSB-BP325979FKZ
CSB-MP325979FKZ
CSB-EP325979FKZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vaccinia virus Protein K5 (K5L) CSB-YP325980VAI
CSB-EP325980VAI
CSB-BP325980VAI
CSB-MP325980VAI
CSB-EP325980VAI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Sensor protein RstB (rstB), partial CSB-YP325981ENV
CSB-EP325981ENV
CSB-BP325981ENV
CSB-MP325981ENV
CSB-EP325981ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse Seminal vesicle secretory protein 4 (Svs4) CSB-YP325982MO
CSB-BP325982MO
CSB-MP325982MO
CSB-EP325982MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila melanogaster Protein kinase shaggy (sgg), partial CSB-YP325983DLU
CSB-EP325983DLU
CSB-BP325983DLU
CSB-MP325983DLU
CSB-EP325983DLU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Porcine transmissible gastroenteritis coronavirus Non-structural protein 3a (3a) CSB-YP325984PQF
CSB-EP325984PQF
CSB-BP325984PQF
CSB-MP325984PQF
CSB-EP325984PQF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human HLA class I histocompatibility antigen, A-25 alpha chain (HLA-A), partial CSB-YP325985HU
CSB-EP325985HU
CSB-BP325985HU
CSB-MP325985HU
CSB-EP325985HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse H-2 class II histocompatibility antigen, I-A beta chain (H2-Eb1), partial CSB-YP325986MO
CSB-EP325986MO
CSB-BP325986MO
CSB-MP325986MO
CSB-EP325986MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Watermelon mosaic virus II Genome polyprotein CSB-YP325987WAC
CSB-EP325987WAC
CSB-BP325987WAC
CSB-MP325987WAC
CSB-EP325987WAC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila melanogaster Protein anon-37Cs (anon-37Cs), partial CSB-YP325988DLU
CSB-EP325988DLU
CSB-BP325988DLU
CSB-MP325988DLU
CSB-EP325988DLU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Nitrogen regulatory protein GLN3 (GLN3), partial CSB-YP325989SVG
CSB-EP325989SVG
CSB-BP325989SVG
CSB-MP325989SVG
CSB-EP325989SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Haloarcula marismortui Ribonuclease P protein component 1 (rnp1) CSB-YP325990HTJ
CSB-EP325990HTJ
CSB-BP325990HTJ
CSB-MP325990HTJ
CSB-EP325990HTJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Heat-stable enterotoxin II (stiI) CSB-YP325991ENL
CSB-EP325991ENL
CSB-BP325991ENL
CSB-MP325991ENL
CSB-EP325991ENL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nitrogenase iron protein 4 (nifH4) CSB-YP325992CMA
CSB-EP325992CMA
CSB-BP325992CMA
CSB-MP325992CMA
CSB-EP325992CMA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neurospora crassa Carbamoyl-phosphate synthase arginine-specific small chain, mitochondrial (cpa-1) CSB-YP325993NHA
CSB-EP325993NHA
CSB-BP325993NHA
CSB-MP325993NHA
CSB-EP325993NHA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Psophocarpus scandens Basic lectin B2 CSB-YP325995PWP
CSB-EP325995PWP
CSB-BP325995PWP
CSB-MP325995PWP
CSB-EP325995PWP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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