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重組蛋白

產品名稱 貨號 規(guī)格
Recombinant Putative peptidyl-prolyl cis-trans isomerase NifM (nifM) CSB-YP326038DPU
CSB-EP326038DPU
CSB-BP326038DPU
CSB-MP326038DPU
CSB-EP326038DPU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila melanogaster Putative ATP-dependent RNA helicase me31b (me31B) CSB-YP326039DLU
CSB-EP326039DLU
CSB-BP326039DLU
CSB-MP326039DLU
CSB-EP326039DLU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptomyces coelicolor Probable acyl carrier protein (SCO5316) CSB-YP326041FOB
CSB-EP326041FOB
CSB-BP326041FOB
CSB-MP326041FOB
CSB-EP326041FOB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 34.2 kDa protein in rubredoxin operon CSB-YP326042CMA
CSB-EP326042CMA
CSB-BP326042CMA
CSB-MP326042CMA
CSB-EP326042CMA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant GTPase HRas (H-RAS) CSB-YP326043MKN
CSB-EP326043MKN
CSB-BP326043MKN
CSB-MP326043MKN
CSB-EP326043MKN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Microcin B17-processing protein mcbB (mcbB) CSB-YP326044ENL
CSB-EP326044ENL
CSB-BP326044ENL
CSB-MP326044ENL
CSB-EP326044ENL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bradyrhizobium japonicum Sensor protein fixL (fixL), partial CSB-YP326045BVW
CSB-EP326045BVW
CSB-BP326045BVW
CSB-MP326045BVW
CSB-EP326045BVW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chlamydomonas reinhardtii Cytochrome b6-f complex subunit 4 (petD), partial CSB-YP326046DSJ1
CSB-EP326046DSJ1
CSB-BP326046DSJ1
CSB-MP326046DSJ1
CSB-EP326046DSJ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Negative RAS protein regulator protein (RPI1) CSB-YP326047SVG
CSB-EP326047SVG
CSB-BP326047SVG
CSB-MP326047SVG
CSB-EP326047SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Protein MalY (malY) CSB-YP326048ENV
CSB-EP326048ENV
CSB-BP326048ENV
CSB-MP326048ENV
CSB-EP326048ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas putida Salicylate hydroxylase (nahG) CSB-YP326049FFZ
CSB-BP326049FFZ
CSB-MP326049FFZ
CSB-EP326049FFZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rat Olfactory receptor 1493 (Olr1493), partial CSB-YP326050RA1
CSB-EP326050RA1
CSB-BP326050RA1
CSB-MP326050RA1
CSB-EP326050RA1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nicotiana tabacum 3-phosphoshikimate 1-carboxyvinyltransferase 2 (EPSPS-2), partial CSB-YP326051NHE
CSB-EP326051NHE
CSB-BP326051NHE
CSB-MP326051NHE
CSB-EP326051NHE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacillus stearothermophilus 50S ribosomal protein L22 (rplV) CSB-YP326052GFM
CSB-EP326052GFM
CSB-BP326052GFM
CSB-MP326052GFM
CSB-EP326052GFM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermosynechococcus vulcanus Photosystem I reaction center subunit PsaK (psaK), partial CSB-YP326053TNI1
CSB-EP326053TNI1
CSB-BP326053TNI1
CSB-MP326053TNI1
CSB-EP326053TNI1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Spinacia oleracea 50S ribosomal protein L35, chloroplastic (RPL35) CSB-YP326054FKI
CSB-EP326054FKI
CSB-BP326054FKI
CSB-MP326054FKI
CSB-EP326054FKI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Daucus carota Actin-1 CSB-YP326055DIR
CSB-EP326055DIR
CSB-BP326055DIR
CSB-MP326055DIR
CSB-EP326055DIR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xanthomonas campestris pv. campestris PTS system fructose-specific EIIBC component (fruA), partial CSB-YP326056XAY
CSB-EP326056XAY
CSB-BP326056XAY
CSB-MP326056XAY
CSB-EP326056XAY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 30S ribosomal protein S6 (rpsF) CSB-YP326057TNR
CSB-EP326057TNR
CSB-BP326057TNR
CSB-MP326057TNR
CSB-EP326057TNR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodobacter capsulatus 1-phosphofructokinase (fruK) CSB-YP326058RLC
CSB-EP326058RLC
CSB-BP326058RLC
CSB-MP326058RLC
CSB-EP326058RLC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質形式,可以通過多種方式產生大量蛋白質、修改基因序列和制造有用的商業(yè)產品。重組蛋白的技術資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據他們開發(fā)重組蛋白的經驗總結出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產,在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質合成機制來產生由該基因編碼的蛋白質。表達載體可使外源基因在宿主細胞中高效表達,經過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術。在重組蛋白生產過程中是一種基本成分。正如標題所示,本文主要總結了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質?

根據重組蛋白的不同應用,對其生物活性有不同的要求。但是我們如何確保蛋白質的產生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務,您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質有任何疑問,涉及到蛋白質的價格、交貨、質量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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