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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Putative transposase for insertion sequence element IS112 CSB-YP326059FNU
CSB-EP326059FNU
CSB-BP326059FNU
CSB-MP326059FNU
CSB-EP326059FNU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cyanelle 30S ribosomal protein S3 (rps3) CSB-YP326060DZX
CSB-EP326060DZX
CSB-BP326060DZX
CSB-MP326060DZX
CSB-EP326060DZX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 50S ribosomal protein L16, cyanelle (rpl16) CSB-YP326061DZX
CSB-EP326061DZX
CSB-BP326061DZX
CSB-MP326061DZX
CSB-EP326061DZX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Solanum lycopersicum Chalcone synthase 1 (CHS1) CSB-YP326062SZY
CSB-EP326062SZY
CSB-BP326062SZY
CSB-MP326062SZY
CSB-EP326062SZY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Maedi visna virus Gag polyprotein (gag) CSB-YP326063MAU
CSB-EP326063MAU
CSB-BP326063MAU
CSB-MP326063MAU
CSB-EP326063MAU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nicotiana tabacum Glucan endo-1,3-beta-glucosidase (SP41A) CSB-YP326064NHE
CSB-EP326064NHE
CSB-BP326064NHE
CSB-MP326064NHE
CSB-EP326064NHE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Probable flagellar assembly protein fliH (fliH) CSB-YP326065BRJ
CSB-EP326065BRJ
CSB-BP326065BRJ
CSB-MP326065BRJ
CSB-EP326065BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodobacter capsulatus Protein pucC (pucC), partial CSB-YP326066RLC
CSB-EP326066RLC
CSB-BP326066RLC
CSB-MP326066RLC
CSB-EP326066RLC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse X-ray repair cross-complementing protein 6 (Xrcc6) CSB-YP326067MO
CSB-EP326067MO
CSB-BP326067MO
CSB-MP326067MO
CSB-EP326067MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila melanogaster Protein midgut expression 1 (mex1) CSB-YP326068DLU
CSB-EP326068DLU
CSB-BP326068DLU
CSB-MP326068DLU
CSB-EP326068DLU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactococcus lactis subsp. lactis Protein lacX, plasmid (lacX) CSB-YP326069LNI
CSB-EP326069LNI
CSB-BP326069LNI
CSB-MP326069LNI
CSB-EP326069LNI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Cytosolic Fe-S cluster assembly factor NAR1 (NAR1) CSB-YP326070SVG
CSB-EP326070SVG
CSB-BP326070SVG
CSB-MP326070SVG
CSB-EP326070SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus carnosus Phosphoenolpyruvate-protein phosphotransferase (ptsI), partial CSB-YP326071FLK
CSB-EP326071FLK
CSB-BP326071FLK
CSB-MP326071FLK
CSB-EP326071FLK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nicotiana tabacum Glucan endo-1,3-beta-glucosidase, basic vacuolar isoform GGIB50 CSB-YP326072NHE
CSB-EP326072NHE
CSB-BP326072NHE
CSB-MP326072NHE
CSB-EP326072NHE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Caldocellum saccharolyticum Acetyl esterase (xynC) CSB-YP326073CAM
CSB-EP326073CAM
CSB-BP326073CAM
CSB-MP326073CAM
CSB-EP326073CAM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Serine/threonine-protein kinase STE11 (STE11), partial CSB-YP326074SVG
CSB-BP326074SVG
CSB-MP326074SVG
CSB-EP326074SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Serine/threonine-protein phosphatase PP2A-1 catalytic subunit (PPH21) CSB-YP326075SVG
CSB-EP326075SVG
CSB-BP326075SVG
CSB-MP326075SVG
CSB-EP326075SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mustela putorius Hemoglobin subunit alpha-1/2 CSB-YP326076MKU
CSB-EP326076MKU
CSB-BP326076MKU
CSB-MP326076MKU
CSB-EP326076MKU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cupriavidus necator Poly-beta-hydroxybutyrate polymerase (phbC), partial CSB-YP326077DZQ
CSB-EP326077DZQ
CSB-BP326077DZQ
CSB-MP326077DZQ
CSB-EP326077DZQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neurospora crassa Sulfate permease 2 (cys-14), partial CSB-YP326078NHA
CSB-EP326078NHA
CSB-BP326078NHA
CSB-MP326078NHA
CSB-EP326078NHA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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