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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Putative membrane protein insertion efficiency factor CSB-YP326349EYY
CSB-EP326349EYY
CSB-BP326349EYY
CSB-MP326349EYY
CSB-EP326349EYY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant DNA replication and repair protein recF (recF) CSB-YP326350EYY
CSB-EP326350EYY
CSB-BP326350EYY
CSB-MP326350EYY
CSB-EP326350EYY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Allochromatium vinosum Ribulose bisphosphate carboxylase large chain 2 (cbbL2) CSB-YP326352AZP
CSB-EP326352AZP
CSB-BP326352AZP
CSB-MP326352AZP
CSB-EP326352AZP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Glycerol operon regulatory protein (gylR) CSB-YP326353SUW
CSB-EP326353SUW
CSB-BP326353SUW
CSB-MP326353SUW
CSB-EP326353SUW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dictyostelium discoideum Nucleoside diphosphate kinase, cytosolic (ndkC-1) CSB-YP326354DKK
CSB-BP326354DKK
CSB-MP326354DKK
CSB-EP326354DKK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Glycine max P34 probable thiol protease CSB-YP326355GGV
CSB-EP326355GGV
CSB-BP326355GGV
CSB-MP326355GGV
CSB-EP326355GGV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Enterobacteria phage T4 Transcription regulatory protein motA (motA) CSB-YP326357EDZ
CSB-EP326357EDZ
CSB-BP326357EDZ
CSB-MP326357EDZ
CSB-EP326357EDZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Petunia hybrida Chalcone synthase B (CHSB) CSB-YP326358EUD
CSB-EP326358EUD
CSB-BP326358EUD
CSB-MP326358EUD
CSB-EP326358EUD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomycopsis fibuligera Acid protease (PEP1) CSB-YP326359SAK
CSB-EP326359SAK
CSB-BP326359SAK
CSB-MP326359SAK
CSB-EP326359SAK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Orientia tsutsugamushi 56 kDa type-specific antigen, partial CSB-YP326360OCJ1
CSB-EP326360OCJ1
CSB-BP326360OCJ1
CSB-MP326360OCJ1
CSB-EP326360OCJ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methyl-coenzyme M reductase subunit beta CSB-YP326361MSO
CSB-EP326361MSO
CSB-BP326361MSO
CSB-MP326361MSO
CSB-EP326361MSO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Tobacco necrosis virus Capsid protein (ORF4) CSB-YP326362TFG
CSB-EP326362TFG
CSB-BP326362TFG
CSB-MP326362TFG
CSB-EP326362TFG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ulex europaeus Anti-H (O) lectin 2 CSB-YP326363UAB
CSB-EP326363UAB
CSB-BP326363UAB
CSB-MP326363UAB
CSB-EP326363UAB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodococcus erythropolis Amidase (amdA), partial CSB-YP326364RLJ
CSB-EP326364RLJ
CSB-BP326364RLJ
CSB-MP326364RLJ
CSB-EP326364RLJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Uncharacterized 28.3 kDa protein in PAR locus CSB-YP326365ENL
CSB-EP326365ENL
CSB-BP326365ENL
CSB-MP326365ENL
CSB-EP326365ENL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Flavodoxin-A CSB-YP326366DPU
CSB-EP326366DPU
CSB-BP326366DPU
CSB-MP326366DPU
CSB-EP326366DPU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudonaja textilis Phospholipase A2, textilotoxin D chain CSB-YP326368FGS
CSB-EP326368FGS
CSB-BP326368FGS
CSB-MP326368FGS
CSB-EP326368FGS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rotavirus A Outer capsid protein VP4, partial CSB-YP326369RGW
CSB-EP326369RGW
CSB-BP326369RGW
CSB-MP326369RGW
CSB-EP326369RGW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Tetracycline resistance leader peptide (tetL) CSB-YP326370BRJ
CSB-EP326370BRJ
CSB-BP326370BRJ
CSB-MP326370BRJ
CSB-EP326370BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pardachirus pavoninus Pardaxin P-2 CSB-YP326371PCV
CSB-EP326371PCV
CSB-BP326371PCV
CSB-MP326371PCV
CSB-EP326371PCV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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