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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Genome polyprotein CSB-YP326329TAO
CSB-EP326329TAO
CSB-BP326329TAO
CSB-MP326329TAO
CSB-EP326329TAO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas aeruginosa Amino-acid acetyltransferase (argA) CSB-YP326330EZX
CSB-EP326330EZX
CSB-BP326330EZX
CSB-MP326330EZX
CSB-EP326330EZX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Probable amidase (AMD2), partial CSB-YP326331SVG
CSB-EP326331SVG
CSB-BP326331SVG
CSB-MP326331SVG
CSB-EP326331SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Odontoglossum ringspot virus Movement protein (MP) CSB-YP326332OAM
CSB-EP326332OAM
CSB-BP326332OAM
CSB-MP326332OAM
CSB-EP326332OAM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Potato virus Y Genome polyprotein, partial CSB-YP326333PRL
CSB-EP326333PRL
CSB-BP326333PRL
CSB-MP326333PRL
CSB-EP326333PRL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Myxoma virus Kelch repeat protein M-T8 (m008L), partial CSB-YP326334MMS
CSB-EP326334MMS
CSB-BP326334MMS
CSB-MP326334MMS
CSB-EP326334MMS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Myxine glutinosa Insulin-like growth factor CSB-YP326335MWW
CSB-EP326335MWW
CSB-BP326335MWW
CSB-MP326335MWW
CSB-EP326335MWW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Protobothrops mucrosquamatus Phospholipase A2, basic CSB-YP326336EZD
CSB-EP326336EZD
CSB-BP326336EZD
CSB-MP326336EZD
CSB-EP326336EZD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Schistocerca americana Fasciclin-2 (FAS2), partial CSB-YP326337SDK
CSB-EP326337SDK
CSB-BP326337SDK
CSB-MP326337SDK
CSB-EP326337SDK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant V-type ATP synthase alpha chain (atpA), partial CSB-YP326338MSL
CSB-EP326338MSL
CSB-BP326338MSL
CSB-MP326338MSL
CSB-EP326338MSL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lithobates catesbeiana Ranatachykinin-D CSB-YP326339LQA
CSB-EP326339LQA
CSB-BP326339LQA
CSB-MP326339LQA
CSB-EP326339LQA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila melanogaster Calcium-transporting ATPase sarcoplasmic/endoplasmic reticulum type (Ca-P60A), partial CSB-YP326340DLU
CSB-EP326340DLU
CSB-BP326340DLU
CSB-MP326340DLU
CSB-EP326340DLU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Multifunctional conjugation protein TraI (traI), partial CSB-YP326341ENL
CSB-EP326341ENL
CSB-BP326341ENL
CSB-MP326341ENL
CSB-EP326341ENL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila melanogaster Muscle-specific homeobox protein tinman (tin) CSB-YP326342DLU
CSB-EP326342DLU
CSB-BP326342DLU
CSB-MP326342DLU
CSB-EP326342DLU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Francolinus pondicerianus Hemoglobin subunit alpha-A (HBAA) CSB-YP326343FCS
CSB-EP326343FCS
CSB-BP326343FCS
CSB-MP326343FCS
CSB-EP326343FCS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Hediste diversicolor Myohemerythrin CSB-YP326344HCK
CSB-EP326344HCK
CSB-BP326344HCK
CSB-MP326344HCK
CSB-EP326344HCK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Thioredoxin-2 (TRX2) CSB-YP326345SVG
CSB-EP326345SVG
CSB-BP326345SVG
CSB-MP326345SVG
CSB-EP326345SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila melanogaster Homeotic protein ocelliless (oc), partial CSB-YP326346DLU
CSB-EP326346DLU
CSB-BP326346DLU
CSB-MP326346DLU
CSB-EP326346DLU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lysinibacillus sphaericus Probable biotin transporter BioY (bioY), partial CSB-YP326347LRU1
CSB-EP326347LRU1
CSB-BP326347LRU1
CSB-MP326347LRU1
CSB-EP326347LRU1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Eristicophis macmahoni Disintegrin eristicophin CSB-YP326348EGH
CSB-EP326348EGH
CSB-BP326348EGH
CSB-MP326348EGH
CSB-EP326348EGH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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