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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Drosophila pseudoobscura pseudoobscura Opsin Rh3 (Rh3), partial CSB-YP326823DME1
CSB-EP326823DME1
CSB-BP326823DME1
CSB-MP326823DME1
CSB-EP326823DME1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Feline calicivirus Protein VP2 (ORF3) CSB-YP326824FAG
CSB-EP326824FAG
CSB-BP326824FAG
CSB-MP326824FAG
CSB-EP326824FAG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Zymomonas mobilis DNA ligase (ligA), partial CSB-YP326825ZBM
CSB-EP326825ZBM
CSB-BP326825ZBM
CSB-MP326825ZBM
CSB-EP326825ZBM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Giardia intestinalis NADP-specific glutamate dehydrogenase CSB-YP326826GFW
CSB-EP326826GFW
CSB-BP326826GFW
CSB-MP326826GFW
CSB-EP326826GFW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Kinesin-like protein KIP1 (KIP1), partial CSB-YP326827SVG
CSB-EP326827SVG
CSB-BP326827SVG
CSB-MP326827SVG
CSB-EP326827SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Glycine max Superoxide dismutase [Fe], chloroplastic (SODB) CSB-YP326828GGV
CSB-EP326828GGV
CSB-BP326828GGV
CSB-MP326828GGV
CSB-EP326828GGV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Fatty acid oxidation complex subunit alpha (fadB), partial CSB-YP326829PUK
CSB-EP326829PUK
CSB-BP326829PUK
CSB-MP326829PUK
CSB-EP326829PUK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae 3-hydroxyisobutyryl-CoA hydrolase, mitochondrial (EHD3), partial CSB-YP326830SVG
CSB-EP326830SVG
CSB-BP326830SVG
CSB-MP326830SVG
CSB-EP326830SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Amsacta moorei entomopoxvirus Uncharacterized protein AMV017/Q1 (AMV017) CSB-YP326831AJE
CSB-EP326831AJE
CSB-BP326831AJE
CSB-MP326831AJE
CSB-EP326831AJE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ictalurid herpesvirus 1 DNA polymerase (57/58), partial CSB-YP326832IAD
CSB-EP326832IAD
CSB-BP326832IAD
CSB-MP326832IAD
CSB-EP326832IAD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human herpesvirus 6A Virion egress protein U37 (U37) CSB-YP326833HJZ
CSB-EP326833HJZ
CSB-BP326833HJZ
CSB-MP326833HJZ
CSB-EP326833HJZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Schizosaccharomyces pombe Plasma membrane ATPase 2 (pma2), partial CSB-YP326834SXV
CSB-EP326834SXV
CSB-BP326834SXV
CSB-MP326834SXV
CSB-EP326834SXV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Conus striatus Omega-conotoxin SVIB CSB-YP326835DWI
CSB-EP326835DWI
CSB-BP326835DWI
CSB-MP326835DWI
CSB-EP326835DWI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Agkistrodon contortrix contortrix Zinc metalloproteinase fibrolase CSB-YP326836AET
CSB-EP326836AET
CSB-BP326836AET
CSB-MP326836AET
CSB-EP326836AET-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Anaerobic ribonucleoside-triphosphate reductase (nrdD) CSB-YP326837ENV
CSB-EP326837ENV
CSB-BP326837ENV
CSB-MP326837ENV
CSB-EP326837ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli H repeat-associated protein yhhI (yhhI) CSB-YP326838ENV
CSB-EP326838ENV
CSB-BP326838ENV
CSB-MP326838ENV
CSB-EP326838ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Equine herpesvirus 1 Serine/threonine-protein kinase US3 homolog (69) CSB-YP326839EFJ
CSB-EP326839EFJ
CSB-BP326839EFJ
CSB-MP326839EFJ
CSB-EP326839EFJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Equine herpesvirus 1 Probable ATP-dependent helicase 57 (57), partial CSB-YP326840EFJ
CSB-EP326840EFJ
CSB-BP326840EFJ
CSB-MP326840EFJ
CSB-EP326840EFJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Equine herpesvirus 1 Nuclear phosphoprotein UL3 homolog (60) CSB-YP326841EFJ
CSB-EP326841EFJ
CSB-BP326841EFJ
CSB-MP326841EFJ
CSB-EP326841EFJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Equine herpesvirus 1 Tegument protein UL14 homolog (48) CSB-YP326842EFJ
CSB-EP326842EFJ
CSB-BP326842EFJ
CSB-MP326842EFJ
CSB-EP326842EFJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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