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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Zea mays Ferritin-2, chloroplastic (FER2) CSB-YP326886ZAX
CSB-EP326886ZAX
CSB-BP326886ZAX
CSB-MP326886ZAX
CSB-EP326886ZAX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermotoga maritima 50S ribosomal protein L1 (rplA) CSB-YP326887TNJ
CSB-EP326887TNJ
CSB-BP326887TNJ
CSB-MP326887TNJ
CSB-EP326887TNJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. japonica Alpha-amylase/subtilisin inhibitor (RASI) CSB-YP326888OFG
CSB-EP326888OFG
CSB-BP326888OFG
CSB-MP326888OFG
CSB-EP326888OFG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pediococcus acidilactici Bacteriocin pediocin PA-1 (pedA) CSB-YP326889PEC
CSB-BP326889PEC
CSB-MP326889PEC
CSB-EP326889PEC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant DNA polymerase III subunit beta (dnaN) CSB-YP326890SYN
CSB-EP326890SYN
CSB-BP326890SYN
CSB-MP326890SYN
CSB-EP326890SYN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nicotiana tabacum Thioredoxin H-type 1 CSB-YP326891NHE
CSB-EP326891NHE
CSB-BP326891NHE
CSB-MP326891NHE
CSB-EP326891NHE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Tyrosine-protein phosphatase 2 (PTP2), partial CSB-YP326892SVG
CSB-EP326892SVG
CSB-BP326892SVG
CSB-MP326892SVG
CSB-EP326892SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio cholerae serotype O1 Toxin coregulated pilus biosynthesis protein T (tcpT), partial CSB-YP326893VEX
CSB-EP326893VEX
CSB-BP326893VEX
CSB-MP326893VEX
CSB-EP326893VEX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio cholerae serotype O1 Toxin coregulated pilus biosynthesis protein D (tcpD), partial CSB-YP326894VEX1
CSB-EP326894VEX1
CSB-BP326894VEX1
CSB-MP326894VEX1
CSB-EP326894VEX1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Tubulin alpha-2/alpha-4 chain (TUBA2) CSB-YP326895DOA
CSB-EP326895DOA
CSB-BP326895DOA
CSB-MP326895DOA
CSB-EP326895DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bombyx mori Bombyxin B-12 (BBXB12) CSB-YP326896BTT
CSB-EP326896BTT
CSB-BP326896BTT
CSB-MP326896BTT
CSB-EP326896BTT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Gossypium hirsutum Oleosin 18.2 kDa (MATP6-A), partial CSB-YP326897GHB1
CSB-EP326897GHB1
CSB-BP326897GHB1
CSB-MP326897GHB1
CSB-EP326897GHB1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Glycine max P24 oleosin isoform B, partial CSB-YP326898GGV1
CSB-EP326898GGV1
CSB-BP326898GGV1
CSB-MP326898GGV1
CSB-EP326898GGV1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Modification methylase HpaI (hpaIM) CSB-YP326899HAL
CSB-EP326899HAL
CSB-BP326899HAL
CSB-MP326899HAL
CSB-EP326899HAL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. japonica Elongation factor 1-beta (Os07g0662500, LOC_Os07g46750) CSB-YP326900OFG
CSB-EP326900OFG
CSB-BP326900OFG
CSB-MP326900OFG
CSB-EP326900OFG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Elongation factor 3 (TEF3), partial CSB-YP326901EXG
CSB-EP326901EXG
CSB-BP326901EXG
CSB-MP326901EXG
CSB-EP326901EXG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Uncharacterized protein ORF5B CSB-YP326902MEV
CSB-EP326902MEV
CSB-BP326902MEV
CSB-MP326902MEV
CSB-EP326902MEV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Uncharacterized protein ORF8 CSB-YP326903MEV
CSB-EP326903MEV
CSB-BP326903MEV
CSB-MP326903MEV
CSB-EP326903MEV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lederbergia lentus Subtilisin Savinase CSB-YP326904BAT
CSB-EP326904BAT
CSB-BP326904BAT
CSB-MP326904BAT
CSB-EP326904BAT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Subtilisin inhibitor-like protein 1 CSB-YP326905SNR
CSB-EP326905SNR
CSB-BP326905SNR
CSB-MP326905SNR
CSB-EP326905SNR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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