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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Arabidopsis thaliana Chaperonin CPN60, mitochondrial (CPN60), partial CSB-YP326866DOA
CSB-EP326866DOA
CSB-BP326866DOA
CSB-MP326866DOA
CSB-EP326866DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pelophylax ridibundus Peptide YY-like CSB-YP326867PEN
CSB-EP326867PEN
CSB-BP326867PEN
CSB-MP326867PEN
CSB-EP326867PEN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli UPF0502 protein YceH (yceH) CSB-YP326868ENV
CSB-EP326868ENV
CSB-BP326868ENV
CSB-MP326868ENV
CSB-EP326868ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 30S ribosomal protein S19 (rpsS) CSB-YP326869ABI
CSB-EP326869ABI
CSB-BP326869ABI
CSB-MP326869ABI
CSB-EP326869ABI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycoplasma hyorhinis Variant surface antigen C (vlpC), partial CSB-YP326870MLR
CSB-EP326870MLR
CSB-BP326870MLR
CSB-MP326870MLR
CSB-EP326870MLR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Protein RecA (recA) CSB-YP326871DPX
CSB-EP326871DPX
CSB-BP326871DPX
CSB-MP326871DPX
CSB-EP326871DPX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rice dwarf virus Movement protein, partial CSB-YP326872REN
CSB-EP326872REN
CSB-BP326872REN
CSB-MP326872REN
CSB-EP326872REN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prunus serotina Amygdalin beta-glucosidase 1 CSB-YP326873EZL
CSB-EP326873EZL
CSB-BP326873EZL
CSB-MP326873EZL
CSB-EP326873EZL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prunus serotina Prunasin beta-glucosidase 2A CSB-YP326874EZL
CSB-EP326874EZL
CSB-BP326874EZL
CSB-MP326874EZL
CSB-EP326874EZL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodobacter sphaeroides Glyceraldehyde-3-phosphate dehydrogenase B (gapB) CSB-YP326875RLE
CSB-EP326875RLE
CSB-BP326875RLE
CSB-MP326875RLE
CSB-EP326875RLE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodobacter sphaeroides Ribulose bisphosphate carboxylase (cbbM) CSB-YP326876RLE
CSB-EP326876RLE
CSB-BP326876RLE
CSB-MP326876RLE
CSB-EP326876RLE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bradyrhizobium japonicum Nitrogen fixation regulation protein fixK (fixK) CSB-YP326877BVW
CSB-EP326877BVW
CSB-BP326877BVW
CSB-MP326877BVW
CSB-EP326877BVW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nicotiana sylvestris Photosystem I reaction center subunit II, chloroplastic (psaD) CSB-YP326878NHD
CSB-EP326878NHD
CSB-BP326878NHD
CSB-MP326878NHD
CSB-EP326878NHD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila melanogaster 14-3-3 protein zeta (14-3-3zeta) CSB-YP326879DLU
CSB-EP326879DLU
CSB-BP326879DLU
CSB-MP326879DLU
CSB-EP326879DLU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus downei Glucosyltransferase-S (gtfS), partial CSB-YP326880SMJ
CSB-EP326880SMJ
CSB-BP326880SMJ
CSB-MP326880SMJ
CSB-EP326880SMJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 50S ribosomal protein L10 (rplJ) CSB-YP326881FNV
CSB-EP326881FNV
CSB-BP326881FNV
CSB-MP326881FNV
CSB-EP326881FNV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila melanogaster Tyrosine-protein phosphatase corkscrew (csw), partial CSB-YP326882DLU
CSB-EP326882DLU
CSB-BP326882DLU
CSB-MP326882DLU
CSB-EP326882DLU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Spinacia oleracea Chloroplast envelope membrane 70 kDa heat shock-related protein (SCE70), partial CSB-YP326883FKI
CSB-EP326883FKI
CSB-BP326883FKI
CSB-MP326883FKI
CSB-EP326883FKI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Bud emergence protein 1 (BEM1), partial CSB-YP326884SVG
CSB-EP326884SVG
CSB-BP326884SVG
CSB-MP326884SVG
CSB-EP326884SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Agamous-like MADS-box protein AGL3 (AGL3) CSB-YP326885DOA
CSB-EP326885DOA
CSB-BP326885DOA
CSB-MP326885DOA
CSB-EP326885DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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