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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Radianthus magnifica Cytolysin-1 CSB-YP348325RJC
CSB-EP348325RJC
CSB-BP348325RJC
CSB-MP348325RJC
CSB-EP348325RJC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Heteractis crispa Cytolysin RTX-A CSB-YP348326HWE
CSB-EP348326HWE
CSB-BP348326HWE
CSB-MP348326HWE
CSB-EP348326HWE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Asparagine--tRNA ligase (asnS) CSB-YP348327CMB
CSB-EP348327CMB
CSB-BP348327CMB
CSB-MP348327CMB
CSB-EP348327CMB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Listeria monocytogenes serovar 1/2a Asparagine--tRNA ligase (asnS) CSB-YP348328LPY
CSB-EP348328LPY
CSB-BP348328LPY
CSB-MP348328LPY
CSB-EP348328LPY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Asparagine--tRNA ligase (asnS) CSB-YP348329YAS
CSB-EP348329YAS
CSB-BP348329YAS
CSB-MP348329YAS
CSB-EP348329YAS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Trp operon repressor (trpR) CSB-YP348330SWW
CSB-EP348330SWW
CSB-BP348330SWW
CSB-MP348330SWW
CSB-EP348330SWW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Uncharacterized protein CPE0383 (CPE0383), partial CSB-YP348331CMB1
CSB-EP348331CMB1
CSB-BP348331CMB1
CSB-MP348331CMB1
CSB-EP348331CMB1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nostoc sp. Cyanate hydratase (cynS) CSB-YP348332NHR
CSB-EP348332NHR
CSB-BP348332NHR
CSB-MP348332NHR
CSB-EP348332NHR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Anthopleura elegantissima Antho-KAamide CSB-YP348333DNA
CSB-EP348333DNA
CSB-BP348333DNA
CSB-MP348333DNA
CSB-EP348333DNA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Anthopleura elegantissima Antho-RNamide CSB-YP348334DNA
CSB-EP348334DNA
CSB-BP348334DNA
CSB-MP348334DNA
CSB-EP348334DNA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant D-arabinitol 4-dehydrogenase (dalD) CSB-YP348335YAS
CSB-EP348335YAS
CSB-BP348335YAS
CSB-MP348335YAS
CSB-EP348335YAS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nostoc sp. 4-hydroxythreonine-4-phosphate dehydrogenase (pdxA) CSB-YP348336NHR
CSB-EP348336NHR
CSB-BP348336NHR
CSB-MP348336NHR
CSB-EP348336NHR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ralstonia solanacearum 4-hydroxythreonine-4-phosphate dehydrogenase (pdxA) CSB-YP348337RAR
CSB-EP348337RAR
CSB-BP348337RAR
CSB-MP348337RAR
CSB-EP348337RAR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 4-hydroxythreonine-4-phosphate dehydrogenase 2 (pdxA2) CSB-YP348338SWW
CSB-EP348338SWW
CSB-BP348338SWW
CSB-MP348338SWW
CSB-EP348338SWW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella typhimurium 4-hydroxythreonine-4-phosphate dehydrogenase 2 (pdxA2) CSB-YP348339SXB
CSB-EP348339SXB
CSB-BP348339SXB
CSB-MP348339SXB
CSB-EP348339SXB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Putative membrane protein igaA homolog (yrfF), partial CSB-YP348340EOD1
CSB-EP348340EOD1
CSB-BP348340EOD1
CSB-MP348340EOD1
CSB-EP348340EOD1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Putative membrane protein igaA homolog (YPO0142, y3922, YP_0143), partial CSB-YP348341YAS1
CSB-EP348341YAS1
CSB-BP348341YAS1
CSB-MP348341YAS1
CSB-EP348341YAS1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Listeria monocytogenes serovar 1/2a Blue-light photoreceptor (lmo0799) CSB-YP348342LPY
CSB-EP348342LPY
CSB-BP348342LPY
CSB-MP348342LPY
CSB-EP348342LPY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella typhimurium Chorismate synthase (aroC) CSB-YP348343SXB
CSB-EP348343SXB
CSB-BP348343SXB
CSB-MP348343SXB
CSB-EP348343SXB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aeropyrum pernix 30S ribosomal protein S14P type Z (rps14p) CSB-YP348344AYB
CSB-EP348344AYB
CSB-BP348344AYB
CSB-MP348344AYB
CSB-EP348344AYB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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