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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Streptococcus pneumoniae 8-oxo-dGTP diphosphatase (mutX) CSB-YP349541SMP
CSB-EP349541SMP
CSB-BP349541SMP
CSB-MP349541SMP
CSB-EP349541SMP-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pneumoniae Glutamyl-tRNA (Gln) amidotransferase subunit C CSB-YP349542SMP
CSB-EP349542SMP
CSB-BP349542SMP
CSB-MP349542SMP
CSB-EP349542SMP-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Malate synthase G (glcB), partial CSB-YP349543SZB
CSB-EP349543SZB
CSB-BP349543SZB
CSB-MP349543SZB
CSB-EP349543SZB-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geodia cydonium Polyubiquitin CSB-YP349544GFU
CSB-EP349544GFU
CSB-BP349544GFU
CSB-MP349544GFU
CSB-EP349544GFU-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Protein fixA (fixA) CSB-YP349545SZB
CSB-EP349545SZB
CSB-BP349545SZB
CSB-MP349545SZB
CSB-EP349545SZB-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Protein fixB (fixB) CSB-YP349546SZB
CSB-EP349546SZB
CSB-BP349546SZB
CSB-MP349546SZB
CSB-EP349546SZB-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chenopodium album Chlorophyllase type 1 CSB-YP349547CIA
CSB-EP349547CIA
CSB-BP349547CIA
CSB-MP349547CIA
CSB-EP349547CIA-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chenopodium album Chlorophyllase type 2 CSB-YP349548CIA
CSB-EP349548CIA
CSB-BP349548CIA
CSB-MP349548CIA
CSB-EP349548CIA-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yarrowia lipolytica 6-phosphofructokinase subunit alpha (PFK1), partial CSB-YP349549YAP
CSB-EP349549YAP
CSB-BP349549YAP
CSB-MP349549YAP
CSB-EP349549YAP-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacillus stearothermophilus 30S ribosomal protein S20 (rpsT) CSB-YP349550GFM
CSB-EP349550GFM
CSB-BP349550GFM
CSB-MP349550GFM
CSB-EP349550GFM-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacillus stearothermophilus 30S ribosomal protein S10 (rpsJ) CSB-YP349551GFM
CSB-EP349551GFM
CSB-BP349551GFM
CSB-MP349551GFM
CSB-EP349551GFM-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Litopenaeus schmitti Crustacean hyperglycemic hormone CSB-YP349552LHB
CSB-EP349552LHB
CSB-BP349552LHB
CSB-MP349552LHB
CSB-EP349552LHB-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Helicobacter pylori Diaminopimelate decarboxylase (lysA) CSB-YP349553HUV
CSB-EP349553HUV
CSB-BP349553HUV
CSB-MP349553HUV
CSB-EP349553HUV-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Helicobacter pylori RNA polymerase sigma-54 factor (rpoN) CSB-YP349554HUV
CSB-EP349554HUV
CSB-BP349554HUV
CSB-MP349554HUV
CSB-EP349554HUV-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Helicobacter pylori Aspartate ammonia-lyase (aspA) CSB-YP349555HUV
CSB-EP349555HUV
CSB-BP349555HUV
CSB-MP349555HUV
CSB-EP349555HUV-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chlorobaculum parvum Histidinol-phosphatase (hisN) CSB-YP349556DSQ
CSB-EP349556DSQ
CSB-BP349556DSQ
CSB-MP349556DSQ
CSB-EP349556DSQ-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Phalaris aquatica Major pollen allergen Pha a 5.4 CSB-YP349557PGU
CSB-EP349557PGU
CSB-BP349557PGU
CSB-MP349557PGU
CSB-EP349557PGU-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Helicobacter pylori Carbon starvation protein A homolog (cstA), partial CSB-YP349559HUV
CSB-EP349559HUV
CSB-BP349559HUV
CSB-MP349559HUV
CSB-EP349559HUV-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vanillyl-alcohol oxidase (VAOA), partial CSB-YP349560ETP
CSB-EP349560ETP
CSB-BP349560ETP
CSB-MP349560ETP
CSB-EP349560ETP-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Danio rerio POU domain, class 3, transcription factor 3-A (pou3f3a) CSB-YP349561DIL
CSB-EP349561DIL
CSB-BP349561DIL
CSB-MP349561DIL
CSB-EP349561DIL-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式，可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中，除了表達(dá)系統(tǒng)之外，還面臨著一系列令人困惑的選擇，如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí)，你必須做出很多決定。如果你選擇的明智，你將獲得高質(zhì)量的重組蛋白，后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定，你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里，來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示，可以幫助你做出明智的決策。

第一條：重組蛋白的生產(chǎn)，在這篇文章中，我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條：如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子，它攜帶特定的基因進(jìn)入宿主細(xì)胞，并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá)，經(jīng)過多年的發(fā)展，表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示，本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條：如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用，對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題，并提供一些解決方案。除了以上文章，我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外，我們還為我們的客戶提供重組蛋白表達(dá)服務(wù)，您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問，涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等，您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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