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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant (3R)-hydroxymyristoyl-[acyl-carrier-protein] dehydratase CSB-YP348525FMW
CSB-EP348525FMW
CSB-BP348525FMW
CSB-MP348525FMW
CSB-EP348525FMW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Maltodextrose utilization protein malA (malA) CSB-YP348526FMW
CSB-EP348526FMW
CSB-BP348526FMW
CSB-MP348526FMW
CSB-EP348526FMW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Putative endo-beta-N-acetylglucosaminidase (lytB), partial CSB-YP348527FMW
CSB-EP348527FMW
CSB-BP348527FMW
CSB-MP348527FMW
CSB-EP348527FMW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pneumoniae Putative endo-beta-N-acetylglucosaminidase (lytB), partial CSB-YP348528SMP
CSB-EP348528SMP
CSB-BP348528SMP
CSB-MP348528SMP
CSB-EP348528SMP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Regulatory protein recX (recX) CSB-YP348529SMH
CSB-EP348529SMH
CSB-BP348529SMH
CSB-MP348529SMH
CSB-EP348529SMH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Regulatory protein recX (recX) CSB-YP348530FOI
CSB-EP348530FOI
CSB-BP348530FOI
CSB-MP348530FOI
CSB-EP348530FOI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Maltose/maltodextrin-binding protein (malX) CSB-YP348531FMW
CSB-EP348531FMW
CSB-BP348531FMW
CSB-MP348531FMW
CSB-EP348531FMW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana 40S ribosomal protein S13-2 (RPS13B) CSB-YP348532DOA
CSB-EP348532DOA
CSB-BP348532DOA
CSB-MP348532DOA
CSB-EP348532DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana 60S ribosomal protein L10a-3 (RPL10AC) CSB-YP348533DOA
CSB-EP348533DOA
CSB-BP348533DOA
CSB-MP348533DOA
CSB-EP348533DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Ubiquitin-40S ribosomal protein S27a-3 (RPS27AC) CSB-YP348534DOA
CSB-EP348534DOA
CSB-BP348534DOA
CSB-MP348534DOA
CSB-EP348534DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Murein tetrapeptide carboxypeptidase (ldcA) CSB-YP348535EGX
CSB-EP348535EGX
CSB-BP348535EGX
CSB-MP348535EGX
CSB-EP348535EGX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Endo-type membrane-bound lytic murein transglycosylase A (emtA) CSB-YP348536EGX
CSB-EP348536EGX
CSB-BP348536EGX
CSB-MP348536EGX
CSB-EP348536EGX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Putative pyrophosphorylase modD (modD) CSB-YP348537EGX
CSB-EP348537EGX
CSB-BP348537EGX
CSB-MP348537EGX
CSB-EP348537EGX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Leptospira interrogans serogroup Icterohaemorrhagiae serovar Lai Glycerol-3-phosphate acyltransferase (plsY), partial CSB-YP348538LOS1
CSB-EP348538LOS1
CSB-BP348538LOS1
CSB-MP348538LOS1
CSB-EP348538LOS1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella oneidensis Glycerol-3-phosphate acyltransferase (plsY), partial CSB-YP348539SGA1
CSB-EP348539SGA1
CSB-BP348539SGA1
CSB-MP348539SGA1
CSB-EP348539SGA1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus epidermidis Glycerol-3-phosphate acyltransferase (plsY), partial CSB-YP348540SLD1
CSB-EP348540SLD1
CSB-BP348540SLD1
CSB-MP348540SLD1
CSB-EP348540SLD1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermosynechococcus elongatus Glycerol-3-phosphate acyltransferase (plsY), partial CSB-YP348541TDQ1
CSB-EP348541TDQ1
CSB-BP348541TDQ1
CSB-MP348541TDQ1
CSB-EP348541TDQ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vespula maculifrons Melittin (MELT) CSB-YP348542VBZ
CSB-EP348542VBZ
CSB-BP348542VBZ
CSB-MP348542VBZ
CSB-EP348542VBZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Protobothrops flavoviridis Phospholipase A2 isozyme PLA-A, basic CSB-YP348543EZB
CSB-EP348543EZB
CSB-BP348543EZB
CSB-MP348543EZB
CSB-EP348543EZB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant UPF0227 protein VV1_2072 (VV1_2072) CSB-YP348544VFI
CSB-EP348544VFI
CSB-BP348544VFI
CSB-MP348544VFI
CSB-EP348544VFI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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