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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Buchnera aphidicola subsp. Schizaphis graminum Phenylalanine--tRNA ligase beta subunit (pheT), partial CSB-YP348465BXE
CSB-EP348465BXE
CSB-BP348465BXE
CSB-MP348465BXE
CSB-EP348465BXE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Buchnera aphidicola subsp. Schizaphis graminum 30S ribosomal protein S7 (rpsG) CSB-YP348466BXE
CSB-EP348466BXE
CSB-BP348466BXE
CSB-MP348466BXE
CSB-EP348466BXE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodobacter capsulatus 30S ribosomal protein S7 (rpsG) CSB-YP348467RLC
CSB-EP348467RLC
CSB-BP348467RLC
CSB-MP348467RLC
CSB-EP348467RLC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermosynechococcus elongatus Formamidopyrimidine-DNA glycosylase (mutM) CSB-YP348468TDQ
CSB-EP348468TDQ
CSB-BP348468TDQ
CSB-MP348468TDQ
CSB-EP348468TDQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Austrelaps superbus Phospholipase A2, superbin a CSB-YP348469DPN
CSB-EP348469DPN
CSB-BP348469DPN
CSB-MP348469DPN
CSB-EP348469DPN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Austrelaps superbus Phospholipase A2, superbin c CSB-YP348470DPN
CSB-EP348470DPN
CSB-BP348470DPN
CSB-MP348470DPN
CSB-EP348470DPN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Daboia russelli russelli Phospholipase A2 VRV-PL-VIIIa CSB-YP348471DAA
CSB-EP348471DAA
CSB-BP348471DAA
CSB-MP348471DAA
CSB-EP348471DAA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudonaja textilis Short neurotoxin N2 CSB-YP348472FGS
CSB-EP348472FGS
CSB-BP348472FGS
CSB-MP348472FGS
CSB-EP348472FGS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Phytophthora infestans GMP reductase CSB-YP348473PJO
CSB-EP348473PJO
CSB-BP348473PJO
CSB-MP348473PJO
CSB-EP348473PJO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chlorobium tepidum Methionine--tRNA ligase (metG), partial CSB-YP348474DST
CSB-EP348474DST
CSB-BP348474DST
CSB-MP348474DST
CSB-EP348474DST-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oceanobacillus iheyensis Methionine--tRNA ligase (metG), partial CSB-YP348475OAB
CSB-EP348475OAB
CSB-BP348475OAB
CSB-MP348475OAB
CSB-EP348475OAB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Allium cepa Lachrymatory-factor synthase (LFS) CSB-YP348476AHI
CSB-EP348476AHI
CSB-BP348476AHI
CSB-MP348476AHI
CSB-EP348476AHI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Anemonia sulcata Antihypertensive protein BDS-2 CSB-YP348477AKE
CSB-EP348477AKE
CSB-BP348477AKE
CSB-MP348477AKE
CSB-EP348477AKE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Buchnera aphidicola subsp. Schizaphis graminum Acetylornithine/succinyldiaminopimelate aminotransferase (argD) CSB-YP348478BXE
CSB-EP348478BXE
CSB-BP348478BXE
CSB-MP348478BXE
CSB-EP348478BXE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Putative uncharacterized protein encoded by LINC00205 (LINC00205) CSB-YP348479HU
CSB-EP348479HU
CSB-BP348479HU
CSB-MP348479HU
CSB-EP348479HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Putative uncharacterized protein encoded by LINC00315 (LINC00315) CSB-YP348480HU
CSB-EP348480HU
CSB-BP348480HU
CSB-MP348480HU
CSB-EP348480HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Trametes hirsuta Pyranose 2-oxidase (P2OX), partial CSB-YP348481THK
CSB-EP348481THK
CSB-BP348481THK
CSB-MP348481THK
CSB-EP348481THK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Buchnera aphidicola subsp. Schizaphis graminum Amino-acid acetyltransferase (argA) CSB-YP348482BXE
CSB-EP348482BXE
CSB-BP348482BXE
CSB-MP348482BXE
CSB-EP348482BXE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oceanobacillus iheyensis Spore cortex-lytic enzyme (sleB) CSB-YP348483OAB
CSB-EP348483OAB
CSB-BP348483OAB
CSB-MP348483OAB
CSB-EP348483OAB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermosynechococcus elongatus Methylenetetrahydrofolate--tRNA- (uracil-5-)-methyltransferase TrmFO CSB-YP348484TDQ
CSB-EP348484TDQ
CSB-BP348484TDQ
CSB-MP348484TDQ
CSB-EP348484TDQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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