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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Human HERV-K_11q22.1 provirus Rec protein CSB-YP350361HU
CSB-EP350361HU
CSB-BP350361HU
CSB-MP350361HU
CSB-EP350361HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human HERV-K_3q12.3 provirus Np9 protein (ERVK-5) CSB-YP350362HU
CSB-EP350362HU
CSB-BP350362HU
CSB-MP350362HU
CSB-EP350362HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant LexA repressor (lexA) CSB-YP350363CQF
CSB-EP350363CQF
CSB-BP350363CQF
CSB-MP350363CQF
CSB-EP350363CQF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant LexA repressor (lexA) CSB-YP350364MLA
CSB-EP350364MLA
CSB-BP350364MLA
CSB-MP350364MLA
CSB-EP350364MLA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodopseudomonas palustris UDP-N-acetylenolpyruvoylglucosamine reductase (murB) CSB-YP350365RLU
CSB-EP350365RLU
CSB-BP350365RLU
CSB-MP350365RLU
CSB-EP350365RLU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Excisionase from transposon Tn916 (xis) CSB-YP350366SMI
CSB-EP350366SMI
CSB-BP350366SMI
CSB-MP350366SMI
CSB-EP350366SMI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pan troglodytes Alpha-2,8-sialyltransferase 8E (ST8SIA5), partial CSB-YP350367EQV
CSB-EP350367EQV
CSB-BP350367EQV
CSB-MP350367EQV
CSB-EP350367EQV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Leptospira interrogans serogroup Icterohaemorrhagiae serovar copenhageni 2-dehydro-3-deoxyphosphooctonate aldolase (kdsA) CSB-YP350368LEV
CSB-EP350368LEV
CSB-BP350368LEV
CSB-MP350368LEV
CSB-EP350368LEV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactobacillus johnsonii DNA mismatch repair protein MutS (mutS), partial CSB-YP350369LAW
CSB-EP350369LAW
CSB-BP350369LAW
CSB-MP350369LAW
CSB-EP350369LAW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacter sulfurreducens UDP-N-acetylmuramate--L-alanine ligase (murC) CSB-YP350370GBK
CSB-EP350370GBK
CSB-BP350370GBK
CSB-MP350370GBK
CSB-EP350370GBK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant UDP-N-acetylmuramate--L-alanine ligase (murC) CSB-YP350371WAQ
CSB-EP350371WAQ
CSB-BP350371WAQ
CSB-MP350371WAQ
CSB-EP350371WAQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Desulfovibrio vulgaris Translation initiation factor IF-1 1 (infA1) CSB-YP350372DDH
CSB-EP350372DDH
CSB-BP350372DDH
CSB-MP350372DDH
CSB-EP350372DDH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Leptospira interrogans serogroup Icterohaemorrhagiae serovar copenhageni Translation initiation factor IF-1 (infA) CSB-YP350373LEV
CSB-EP350373LEV
CSB-BP350373LEV
CSB-MP350373LEV
CSB-EP350373LEV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Translation initiation factor IF-1 (infA) CSB-YP350374SJV
CSB-EP350374SJV
CSB-BP350374SJV
CSB-MP350374SJV
CSB-EP350374SJV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Alanine--tRNA ligase (alaS), partial CSB-YP350375BQN
CSB-EP350375BQN
CSB-BP350375BQN
CSB-MP350375BQN
CSB-EP350375BQN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Desulfovibrio vulgaris Alanine--tRNA ligase (alaS), partial CSB-YP350376DDH
CSB-EP350376DDH
CSB-BP350376DDH
CSB-MP350376DDH
CSB-EP350376DDH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodopseudomonas palustris Alanine--tRNA ligase (alaS), partial CSB-YP350377RLU
CSB-EP350377RLU
CSB-BP350377RLU
CSB-MP350377RLU
CSB-EP350377RLU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus maripaludis Alanine--tRNA ligase (alaS), partial CSB-YP350378MSB
CSB-EP350378MSB
CSB-BP350378MSB
CSB-MP350378MSB
CSB-EP350378MSB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli 6,7-dimethyl-8-ribityllumazine synthase (ribH) CSB-YP350379ENV
CSB-EP350379ENV
CSB-BP350379ENV
CSB-MP350379ENV
CSB-EP350379ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus 6,7-dimethyl-8-ribityllumazine synthase (ribH) CSB-YP350380BQN
CSB-EP350380BQN
CSB-BP350380BQN
CSB-MP350380BQN
CSB-EP350380BQN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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