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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Arabidopsis thaliana Probable disease resistance protein At1g12280 (At1g12280), partial CSB-YP350279DOA
CSB-EP350279DOA
CSB-BP350279DOA
CSB-MP350279DOA
CSB-EP350279DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O6:H1 Aquaporin Z (aqpZ), partial CSB-YP350280EGX1
CSB-EP350280EGX1
CSB-BP350280EGX1
CSB-MP350280EGX1
CSB-EP350280EGX1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nanoarchaeum equitans DNA/RNA-binding protein Alba (albA) CSB-YP350281NAQ
CSB-EP350281NAQ
CSB-BP350281NAQ
CSB-MP350281NAQ
CSB-EP350281NAQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Glucose-specific phosphotransferase enzyme IIA component (crr) CSB-YP350282SKY
CSB-EP350282SKY
CSB-BP350282SKY
CSB-MP350282SKY
CSB-EP350282SKY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Metallothiol transferase fosB (fosB) CSB-YP350283SKY
CSB-EP350283SKY
CSB-BP350283SKY
CSB-MP350283SKY
CSB-EP350283SKY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Putative transport protein YbjL (ybjL), partial CSB-YP350284EOD
CSB-EP350284EOD
CSB-BP350284EOD
CSB-MP350284EOD
CSB-EP350284EOD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Histidine--tRNA ligase (hisS) CSB-YP350285ENV
CSB-EP350285ENV
CSB-BP350285ENV
CSB-MP350285ENV
CSB-EP350285ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bdellovibrio bacteriovorus Histidine--tRNA ligase (hisS) CSB-YP350286BFP
CSB-EP350286BFP
CSB-BP350286BFP
CSB-MP350286BFP
CSB-EP350286BFP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodopseudomonas palustris Histidine--tRNA ligase (hisS), partial CSB-YP350287RLU
CSB-EP350287RLU
CSB-BP350287RLU
CSB-MP350287RLU
CSB-EP350287RLU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacter sulfurreducens Glycerol-3-phosphate acyltransferase (plsY), partial CSB-YP350288GBK1
CSB-EP350288GBK1
CSB-BP350288GBK1
CSB-MP350288GBK1
CSB-EP350288GBK1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Undecaprenyl-diphosphatase (uppP), partial CSB-YP350289EOD1
CSB-EP350289EOD1
CSB-BP350289EOD1
CSB-MP350289EOD1
CSB-EP350289EOD1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodopseudomonas palustris Undecaprenyl-diphosphatase (uppP), partial CSB-YP350290RLU1
CSB-EP350290RLU1
CSB-BP350290RLU1
CSB-MP350290RLU1
CSB-EP350290RLU1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Putative ribose uptake protein rbsU (rbsU), partial CSB-YP350291SKY
CSB-EP350291SKY
CSB-BP350291SKY
CSB-MP350291SKY
CSB-EP350291SKY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Prolipoprotein diacylglyceryl transferase (lgt), partial CSB-YP350292ENV1
CSB-EP350292ENV1
CSB-BP350292ENV1
CSB-MP350292ENV1
CSB-EP350292ENV1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Prolipoprotein diacylglyceryl transferase (lgt), partial CSB-YP350293SKY1
CSB-EP350293SKY1
CSB-BP350293SKY1
CSB-MP350293SKY1
CSB-EP350293SKY1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prolipoprotein diacylglyceryl transferase (lgt), partial CSB-YP350294MLA1
CSB-EP350294MLA1
CSB-BP350294MLA1
CSB-MP350294MLA1
CSB-EP350294MLA1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Calisoga sp. U1-nemetoxin-Csp1b CSB-YP350295CAP
CSB-EP350295CAP
CSB-BP350295CAP
CSB-MP350295CAP
CSB-EP350295CAP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pan troglodytes Prolactin-inducible protein homolog (PIP) CSB-YP350296EQV
CSB-EP350296EQV
CSB-BP350296EQV
CSB-MP350296EQV
CSB-EP350296EQV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant His operon leader peptide (hisL) CSB-YP350297EGX
CSB-EP350297EGX
CSB-BP350297EGX
CSB-MP350297EGX
CSB-EP350297EGX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Histidinol-phosphate aminotransferase (hisC) CSB-YP350298MLA
CSB-EP350298MLA
CSB-BP350298MLA
CSB-MP350298MLA
CSB-EP350298MLA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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