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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Treponema denticola Chaperone protein htpG (htpG), partial CSB-YP350319TPP
CSB-EP350319TPP
CSB-BP350319TPP
CSB-MP350319TPP
CSB-EP350319TPP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus maripaludis 30S ribosomal protein S24e (rps24e) CSB-YP350320MSB
CSB-EP350320MSB
CSB-BP350320MSB
CSB-MP350320MSB
CSB-EP350320MSB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Phoneutria nigriventer U1-ctenitoxin-Pn1a CSB-YP350321EUV
CSB-EP350321EUV
CSB-BP350321EUV
CSB-MP350321EUV
CSB-EP350321EUV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus maripaludis Peptidyl-tRNA hydrolase (pth) CSB-YP350322MSB
CSB-EP350322MSB
CSB-BP350322MSB
CSB-MP350322MSB
CSB-EP350322MSB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pyrococcus horikoshii 30S ribosomal protein S27ae (rps27ae) CSB-YP350323FHX
CSB-EP350323FHX
CSB-BP350323FHX
CSB-MP350323FHX
CSB-EP350323FHX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ribulose bisphosphate carboxylase large chain (rbcL) CSB-YP350325MPP
CSB-EP350325MPP
CSB-BP350325MPP
CSB-MP350325MPP
CSB-EP350325MPP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Alligator mississippiensis Somatostatin-14 (SST) CSB-YP350326AZO
CSB-EP350326AZO
CSB-BP350326AZO
CSB-MP350326AZO
CSB-EP350326AZO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Outer-membrane lipoprotein carrier protein (lolA) CSB-YP350327ENV
CSB-EP350327ENV
CSB-BP350327ENV
CSB-MP350327ENV
CSB-EP350327ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Outer-membrane lipoprotein carrier protein (lolA) CSB-YP350328EOD
CSB-EP350328EOD
CSB-BP350328EOD
CSB-MP350328EOD
CSB-EP350328EOD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacter sulfurreducens HPr kinase/phosphorylase (hprK) CSB-YP350329GBK
CSB-EP350329GBK
CSB-BP350329GBK
CSB-MP350329GBK
CSB-EP350329GBK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacter sulfurreducens Aspartyl/glutamyl-tRNA (Asn/Gln) amidotransferase subunit B CSB-YP350330GBK
CSB-EP350330GBK
CSB-BP350330GBK
CSB-MP350330GBK
CSB-EP350330GBK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus maripaludis Aspartyl/glutamyl-tRNA (Asn/Gln) amidotransferase subunit B CSB-YP350331MSB
CSB-EP350331MSB
CSB-BP350331MSB
CSB-MP350331MSB
CSB-EP350331MSB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Protein CrcB homolog 2 (crcB2), partial CSB-YP350332SKX1
CSB-EP350332SKX1
CSB-BP350332SKX1
CSB-MP350332SKX1
CSB-EP350332SKX1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Protein CrcB homolog 1 (crcB1), partial CSB-YP350333MLA1
CSB-EP350333MLA1
CSB-BP350333MLA1
CSB-MP350333MLA1
CSB-EP350333MLA1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Aspartate--ammonia ligase (asnA) CSB-YP350334BQN
CSB-EP350334BQN
CSB-BP350334BQN
CSB-MP350334BQN
CSB-EP350334BQN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus maripaludis Glutamyl-tRNA (Gln) amidotransferase subunit D CSB-YP350335MSB
CSB-EP350335MSB
CSB-BP350335MSB
CSB-MP350335MSB
CSB-EP350335MSB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacter sulfurreducens UPF0313 protein GSU2873 (GSU2873), partial CSB-YP350336GBK
CSB-EP350336GBK
CSB-BP350336GBK
CSB-MP350336GBK
CSB-EP350336GBK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Grammostola rosea Kappa-theraphotoxin-Gr2c CSB-YP350337GDE
CSB-EP350337GDE
CSB-BP350337GDE
CSB-MP350337GDE
CSB-EP350337GDE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Phosphomethylpyrimidine synthase (thiC), partial CSB-YP350338CQF
CSB-EP350338CQF
CSB-BP350338CQF
CSB-MP350338CQF
CSB-EP350338CQF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Desulfovibrio vulgaris Periplasmic [NiFe] hydrogenase small subunit 2 (hynB2) CSB-YP350339DDH
CSB-EP350339DDH
CSB-BP350339DDH
CSB-MP350339DDH
CSB-EP350339DDH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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