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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Bdellovibrio bacteriovorus 6,7-dimethyl-8-ribityllumazine synthase (ribH) CSB-YP352830BFP
CSB-EP352830BFP
CSB-BP352830BFP
CSB-MP352830BFP
CSB-EP352830BFP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 6,7-dimethyl-8-ribityllumazine synthase (ribH) CSB-YP352831MLA
CSB-EP352831MLA
CSB-BP352831MLA
CSB-MP352831MLA
CSB-EP352831MLA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus maripaludis 6,7-dimethyl-8-ribityllumazine synthase (ribH) CSB-YP352832MSB
CSB-EP352832MSB
CSB-BP352832MSB
CSB-MP352832MSB
CSB-EP352832MSB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacter sulfurreducens L-seryl-tRNA (Sec) selenium transferase CSB-YP352833GBK
CSB-EP352833GBK
CSB-BP352833GBK
CSB-MP352833GBK
CSB-EP352833GBK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactobacillus johnsonii Glycerol-3-phosphate dehydrogenase [NAD (P)+] CSB-YP352834LAW
CSB-EP352834LAW
CSB-BP352834LAW
CSB-MP352834LAW
CSB-EP352834LAW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Glycerol-3-phosphate dehydrogenase 2 [NAD (P)+] CSB-YP352835MLA
CSB-EP352835MLA
CSB-BP352835MLA
CSB-MP352835MLA
CSB-EP352835MLA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermus thermophilus Glycerol-3-phosphate dehydrogenase [NAD (P)+] CSB-YP352836TNS
CSB-EP352836TNS
CSB-BP352836TNS
CSB-MP352836TNS
CSB-EP352836TNS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Probable disulfide formation protein C 1 (bdbC1), partial CSB-YP352837BQN1
CSB-EP352837BQN1
CSB-BP352837BQN1
CSB-MP352837BQN1
CSB-EP352837BQN1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermus thermophilus Imidazole glycerol phosphate synthase subunit HisH (hisH) CSB-YP352838TNS
CSB-EP352838TNS
CSB-BP352838TNS
CSB-MP352838TNS
CSB-EP352838TNS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Protein CrcB homolog 2 (crcB2), partial CSB-YP352839CQF1
CSB-EP352839CQF1
CSB-BP352839CQF1
CSB-MP352839CQF1
CSB-EP352839CQF1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant RNA pyrophosphohydrolase (rppH) CSB-YP352840WAQ
CSB-EP352840WAQ
CSB-BP352840WAQ
CSB-MP352840WAQ
CSB-EP352840WAQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Heteropoda venatoria Kappa-sparatoxin-Hv1e CSB-YP352841HGT
CSB-EP352841HGT
CSB-BP352841HGT
CSB-MP352841HGT
CSB-EP352841HGT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus maripaludis Probable cobalt-precorrin-6Y C (15)-methyltransferase [decarboxylating] CSB-YP352842MSB
CSB-EP352842MSB
CSB-BP352842MSB
CSB-MP352842MSB
CSB-EP352842MSB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae ATP synthase subunit 9, mitochondrial (OLI1), partial CSB-YP352843SVG1
CSB-EP352843SVG1
CSB-BP352843SVG1
CSB-MP352843SVG1
CSB-EP352843SVG1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Penicillium funiculosum Histone H3 (HHT1) CSB-YP352844PEX
CSB-EP352844PEX
CSB-BP352844PEX
CSB-MP352844PEX
CSB-EP352844PEX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Photosystem II reaction center protein T (psbT), partial CSB-YP352845DOA
CSB-EP352845DOA
CSB-BP352845DOA
CSB-MP352845DOA
CSB-EP352845DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brassica rapa 50S ribosomal protein L32, chloroplastic (rpl32) CSB-YP352846BWH
CSB-EP352846BWH
CSB-BP352846BWH
CSB-MP352846BWH
CSB-EP352846BWH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhizopus oryzae Lipase CSB-YP352847RDE
CSB-EP352847RDE
CSB-BP352847RDE
CSB-MP352847RDE
CSB-EP352847RDE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pyrococcus furiosus DNA polymerase (pol), partial CSB-YP352848FHW
CSB-EP352848FHW
CSB-BP352848FHW
CSB-MP352848FHW
CSB-EP352848FHW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanosarcina acetivorans 30S ribosomal protein S27e (rps27e) CSB-YP352851MFB
CSB-EP352851MFB
CSB-BP352851MFB
CSB-MP352851MFB
CSB-EP352851MFB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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