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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Brucella melitensis biotype 1 DNA mismatch repair protein mutL (mutL), partial CSB-YP354547BMO
CSB-EP354547BMO
CSB-BP354547BMO
CSB-MP354547BMO
CSB-EP354547BMO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus DNA mismatch repair protein MutS (mutS), partial CSB-YP354548SKY
CSB-EP354548SKY
CSB-BP354548SKY
CSB-MP354548SKY
CSB-EP354548SKY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus MutS2 protein (mutS2), partial CSB-YP354549SKX
CSB-EP354549SKX
CSB-BP354549SKX
CSB-MP354549SKX
CSB-EP354549SKX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant L-aspartate oxidase (nadB), partial CSB-YP354550MVZ
CSB-EP354550MVZ
CSB-BP354550MVZ
CSB-MP354550MVZ
CSB-EP354550MVZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus NH (3)-dependent NAD (+) synthetase CSB-YP354551SKX
CSB-EP354551SKX
CSB-BP354551SKX
CSB-MP354551SKX
CSB-EP354551SKX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant NH (3)-dependent NAD (+) synthetase CSB-YP354552FMW
CSB-EP354552FMW
CSB-BP354552FMW
CSB-MP354552FMW
CSB-EP354552FMW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Putative N-acetylmannosamine-6-phosphate 2-epimerase (nanE) CSB-YP354553SKX
CSB-EP354553SKX
CSB-BP354553SKX
CSB-MP354553SKX
CSB-EP354553SKX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Putative N-acetylmannosamine-6-phosphate 2-epimerase (nanE) CSB-YP354554SMH
CSB-EP354554SMH
CSB-BP354554SMH
CSB-MP354554SMH
CSB-EP354554SMH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Putative N-acetylmannosamine-6-phosphate 2-epimerase 2 (nanE2) CSB-YP354555FMW
CSB-EP354555FMW
CSB-BP354555FMW
CSB-MP354555FMW
CSB-EP354555FMW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Putative N-acetylmannosamine-6-phosphate 2-epimerase (nanE) CSB-YP354556SMT
CSB-EP354556SMT
CSB-BP354556SMT
CSB-MP354556SMT
CSB-EP354556SMT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cell volume regulation protein A (cvrA), partial CSB-YP354557SWW
CSB-EP354557SWW
CSB-BP354557SWW
CSB-MP354557SWW
CSB-EP354557SWW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella typhimurium Electron transport complex protein RnfA (rnfA), partial CSB-YP354558SXB1
CSB-EP354558SXB1
CSB-BP354558SXB1
CSB-MP354558SXB1
CSB-EP354558SXB1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella melitensis biotype 1 Probable manganese transport protein MntH (mntH), partial CSB-YP354559BMO
CSB-EP354559BMO
CSB-BP354559BMO
CSB-MP354559BMO
CSB-EP354559BMO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Protein nrdI (nrdI) CSB-YP354560MVH
CSB-EP354560MVH
CSB-BP354560MVH
CSB-MP354560MVH
CSB-EP354560MVH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Uncharacterized Nudix hydrolase yfcD (yfcD) CSB-YP354561EGX
CSB-EP354561EGX
CSB-BP354561EGX
CSB-MP354561EGX
CSB-EP354561EGX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Uncharacterized Nudix hydrolase yfcD (yfcD) CSB-YP354562SWW
CSB-EP354562SWW
CSB-BP354562SWW
CSB-MP354562SWW
CSB-EP354562SWW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus N utilization substance protein B homolog (nusB) CSB-YP354563SKY
CSB-EP354563SKY
CSB-BP354563SKY
CSB-MP354563SKY
CSB-EP354563SKY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant N utilization substance protein B homolog (nusB) CSB-YP354564SMI
CSB-EP354564SMI
CSB-BP354564SMI
CSB-MP354564SMI
CSB-EP354564SMI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant N utilization substance protein B homolog (nusB) CSB-YP354565SMU
CSB-EP354565SMU
CSB-BP354565SMU
CSB-MP354565SMU
CSB-EP354565SMU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neisseria meningitidis serogroup B Transcription antitermination protein NusG (nusG) CSB-YP354566NGG
CSB-EP354566NGG
CSB-BP354566NGG
CSB-MP354566NGG
CSB-EP354566NGG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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