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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant 4-diphosphocytidyl-2-C-methyl-D-erythritol kinase (ispE) CSB-YP354487MVH
CSB-EP354487MVH
CSB-BP354487MVH
CSB-MP354487MVH
CSB-EP354487MVH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase (ispF) CSB-YP354488MVZ
CSB-EP354488MVZ
CSB-BP354488MVZ
CSB-MP354488MVZ
CSB-EP354488MVZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase (ispF) CSB-YP354489MVH
CSB-EP354489MVH
CSB-BP354489MVH
CSB-MP354489MVH
CSB-EP354489MVH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neisseria meningitidis serogroup B 4-hydroxy-3-methylbut-2-enyl diphosphate reductase (ispH) CSB-YP354490NGG
CSB-EP354490NGG
CSB-BP354490NGG
CSB-MP354490NGG
CSB-EP354490NGG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neisseria meningitidis serogroup A / serotype 4A Probable intracellular septation protein A (NMA2145), partial CSB-YP354491NGF1
CSB-EP354491NGF1
CSB-BP354491NGF1
CSB-MP354491NGF1
CSB-EP354491NGF1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bordetella pertussis Alcaligin biosynthesis enzyme (alcA) CSB-YP354492BUA
CSB-EP354492BUA
CSB-BP354492BUA
CSB-MP354492BUA
CSB-EP354492BUA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Adenylate kinase (adk) CSB-YP354493SKX
CSB-EP354493SKX
CSB-BP354493SKX
CSB-MP354493SKX
CSB-EP354493SKX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Putative ATP:guanido phosphotransferase SAV0524 (SAV0524) CSB-YP354494SKX
CSB-EP354494SKX
CSB-BP354494SKX
CSB-MP354494SKX
CSB-EP354494SKX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Potassium-transporting ATPase C chain (kdpC), partial CSB-YP354495MVZ
CSB-EP354495MVZ
CSB-BP354495MVZ
CSB-MP354495MVZ
CSB-EP354495MVZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella typhimurium 2-dehydro-3-deoxyphosphooctonate aldolase (kdsA) CSB-YP354496SXB
CSB-EP354496SXB
CSB-BP354496SXB
CSB-MP354496SXB
CSB-EP354496SXB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Homoserine kinase (thrB) CSB-YP354497MVZ
CSB-EP354497MVZ
CSB-BP354497MVZ
CSB-MP354497MVZ
CSB-EP354497MVZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Homoserine kinase (thrB) CSB-YP354498SWW
CSB-EP354498SWW
CSB-BP354498SWW
CSB-MP354498SWW
CSB-EP354498SWW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Ribose-phosphate pyrophosphokinase (prs) CSB-YP354499SUL
CSB-EP354499SUL
CSB-BP354499SUL
CSB-MP354499SUL
CSB-EP354499SUL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant L-lactate permease (lldP), partial CSB-YP354500EOD
CSB-EP354500EOD
CSB-BP354500EOD
CSB-MP354500EOD
CSB-EP354500EOD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus L-lactate dehydrogenase 1 (ldhA) CSB-YP354501SKX
CSB-BP354501SKX
CSB-MP354501SKX
CSB-EP354501SKX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neisseria meningitidis serogroup A / serotype 4A Lipoprotein signal peptidase (lspA), partial CSB-YP354502NGF1
CSB-EP354502NGF1
CSB-BP354502NGF1
CSB-MP354502NGF1
CSB-EP354502NGF1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Lipoprotein signal peptidase (lspA), partial CSB-YP354503SKY1
CSB-EP354503SKY1
CSB-BP354503SKY1
CSB-MP354503SKY1
CSB-EP354503SKY1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Elongation factor 4 (lepA), partial CSB-YP354504SMH
CSB-EP354504SMH
CSB-BP354504SMH
CSB-MP354504SMH
CSB-EP354504SMH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Uncharacterized lipoprotein ygdI (ygdI) CSB-YP354505EGX
CSB-EP354505EGX
CSB-BP354505EGX
CSB-MP354505EGX
CSB-EP354505EGX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Uncharacterized lipoprotein ygdR (ygdR) CSB-YP354506ENV
CSB-EP354506ENV
CSB-BP354506ENV
CSB-MP354506ENV
CSB-EP354506ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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