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重組蛋白

產(chǎn)品名稱(chēng) 貨號(hào) 規(guī)格
Recombinant Bacillus subtilis Indole-3-glycerol phosphate synthase (trpC) CSB-YP356176BRJ
CSB-EP356176BRJ
CSB-BP356176BRJ
CSB-MP356176BRJ
CSB-EP356176BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse Ig kappa chain V-II region 17S29.1 CSB-YP356177MO
CSB-EP356177MO
CSB-BP356177MO
CSB-MP356177MO
CSB-EP356177MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse Ig heavy chain V region TEPC 1017 CSB-YP356178MO
CSB-EP356178MO
CSB-BP356178MO
CSB-MP356178MO
CSB-EP356178MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Caiman crocodilus Ig heavy chain V region G4 (G4) CSB-YP356179CAF
CSB-EP356179CAF
CSB-BP356179CAF
CSB-MP356179CAF
CSB-EP356179CAF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rabbit Ig mu chain C region secreted form CSB-YP356180RB
CSB-EP356180RB
CSB-BP356180RB
CSB-MP356180RB
CSB-EP356180RB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nicotiana alata Ribonuclease S-2 (S-2) CSB-YP356181NDF
CSB-EP356181NDF
CSB-BP356181NDF
CSB-MP356181NDF
CSB-EP356181NDF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant B-lymphotropic polyomavirus Major capsid protein VP1 CSB-YP356182BAA
CSB-EP356182BAA
CSB-BP356182BAA
CSB-MP356182BAA
CSB-EP356182BAA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human papillomavirus type 11 Major capsid protein L1 (L1), partial CSB-YP356183HMG
CSB-EP356183HMG
CSB-BP356183HMG
CSB-MP356183HMG
CSB-EP356183HMG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human papillomavirus type 11 Probable protein E5A CSB-YP356184HMG
CSB-EP356184HMG
CSB-BP356184HMG
CSB-MP356184HMG
CSB-EP356184HMG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vaccinia virus Major envelope protein (VACWR052) CSB-YP356185VAI
CSB-BP356185VAI
CSB-MP356185VAI
CSB-EP356185VAI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Protease (pro) CSB-YP356186SHM
CSB-EP356186SHM
CSB-BP356186SHM
CSB-MP356186SHM
CSB-EP356186SHM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Simian retrovirus SRV-1 Envelope glycoprotein (env), partial CSB-BP356187SHM
CSB-MP356187SHM
CSB-EP356187SHM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Dihydrodipicolinate reductase (dapB) CSB-YP356188ENV
CSB-EP356188ENV
CSB-BP356188ENV
CSB-MP356188ENV
CSB-EP356188ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella typhimurium Chemotaxis response regulator protein-glutamate methylesterase (cheB) CSB-YP356189SXB
CSB-EP356189SXB
CSB-BP356189SXB
CSB-MP356189SXB
CSB-EP356189SXB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Feline sarcoma virus Tyrosine-protein kinase transforming protein kit (V-KIT) CSB-YP356190FAZ
CSB-EP356190FAZ
CSB-BP356190FAZ
CSB-MP356190FAZ
CSB-EP356190FAZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Endo-beta-N-acetylglucosaminidase H CSB-EP356191SPF
CSB-BP356191SPF
CSB-MP356191SPF
CSB-EP356191SPF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermitase CSB-YP356192TCT
CSB-EP356192TCT
CSB-BP356192TCT
CSB-MP356192TCT
CSB-EP356192TCT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Tyrosine--tRNA ligase (tyrS) CSB-YP356193BAJ
CSB-EP356193BAJ
CSB-BP356193BAJ
CSB-MP356193BAJ
CSB-EP356193BAJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vipera ammodytes meridionalis Phospholipase A2 inhibitor CSB-YP356194VDE
CSB-EP356194VDE
CSB-BP356194VDE
CSB-MP356194VDE
CSB-EP356194VDE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lophius americanus Glucagon-2 (gcg2) CSB-YP356195LIF
CSB-EP356195LIF
CSB-BP356195LIF
CSB-MP356195LIF
CSB-EP356195LIF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶(hù)提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€(xiàn)留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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