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重組蛋白

產(chǎn)品名稱(chēng) 貨號(hào) 規(guī)格
Recombinant Bifunctional protein pyrR (pyrR) CSB-YP355215FMW
CSB-EP355215FMW
CSB-BP355215FMW
CSB-MP355215FMW
CSB-EP355215FMW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus S-adenosylmethionine:tRNA ribosyltransferase-isomerase (queA) CSB-YP355216SKX
CSB-EP355216SKX
CSB-BP355216SKX
CSB-MP355216SKX
CSB-EP355216SKX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella melitensis biotype 1 UPF0758 protein BMEI0718 (BMEI0718) CSB-YP355217BMO
CSB-EP355217BMO
CSB-BP355217BMO
CSB-MP355217BMO
CSB-EP355217BMO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant UPF0758 protein yicR (yicR) CSB-YP355218EOD
CSB-EP355218EOD
CSB-BP355218EOD
CSB-MP355218EOD
CSB-EP355218EOD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant UPF0758 protein SPy_1118/M5005_Spy0840 (SPy_1118, M5005_Spy0840) CSB-YP355219SMT
CSB-EP355219SMT
CSB-BP355219SMT
CSB-MP355219SMT
CSB-EP355219SMT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Ribosome-binding factor A (rbfA) CSB-YP355220SKX
CSB-EP355220SKX
CSB-BP355220SKX
CSB-MP355220SKX
CSB-EP355220SKX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ribosome-binding factor A (rbfA) CSB-YP355221SMI
CSB-EP355221SMI
CSB-BP355221SMI
CSB-MP355221SMI
CSB-EP355221SMI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella melitensis biotype 1 Protein RecA (recA) CSB-YP355222BMO
CSB-EP355222BMO
CSB-BP355222BMO
CSB-MP355222BMO
CSB-EP355222BMO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Protein RecA (recA) CSB-YP355223SMH
CSB-EP355223SMH
CSB-BP355223SMH
CSB-MP355223SMH
CSB-EP355223SMH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant DNA repair protein recO (recO) CSB-YP355224MVZ
CSB-EP355224MVZ
CSB-BP355224MVZ
CSB-MP355224MVZ
CSB-EP355224MVZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Recombination protein RecR (recR) CSB-YP355225MVZ
CSB-EP355225MVZ
CSB-BP355225MVZ
CSB-MP355225MVZ
CSB-EP355225MVZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Recombination protein RecR (recR) CSB-YP355226SMT
CSB-EP355226SMT
CSB-BP355226SMT
CSB-MP355226SMT
CSB-EP355226SMT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Regulatory protein recX (recX) CSB-YP355227EGX
CSB-EP355227EGX
CSB-BP355227EGX
CSB-MP355227EGX
CSB-EP355227EGX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Regulatory protein recX (recX) CSB-YP355228SKY
CSB-EP355228SKY
CSB-BP355228SKY
CSB-MP355228SKY
CSB-EP355228SKY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Probable soluble pyridine nucleotide transhydrogenase (sthA) CSB-YP355229MVH
CSB-EP355229MVH
CSB-BP355229MVH
CSB-MP355229MVH
CSB-EP355229MVH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Soluble pyridine nucleotide transhydrogenase (sthA) CSB-YP355230SWW
CSB-EP355230SWW
CSB-BP355230SWW
CSB-MP355230SWW
CSB-EP355230SWW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Peptide chain release factor 3 (prfC), partial CSB-YP355231SMT
CSB-EP355231SMT
CSB-BP355231SMT
CSB-MP355231SMT
CSB-EP355231SMT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Peptide chain release factor 2 (prfB) CSB-YP355232MVH
CSB-EP355232MVH
CSB-BP355232MVH
CSB-MP355232MVH
CSB-EP355232MVH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant GTP cyclohydrolase-2 (ribA) CSB-YP355233SWW
CSB-EP355233SWW
CSB-BP355233SWW
CSB-MP355233SWW
CSB-EP355233SWW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 6,7-dimethyl-8-ribityllumazine synthase (ribH) CSB-YP355234MVZ
CSB-EP355234MVZ
CSB-BP355234MVZ
CSB-MP355234MVZ
CSB-EP355234MVZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶(hù)提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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