Your Good Partner in Biology Research

重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Pseudomonas putida Transcriptional regulatory protein xylR (xylR), partial CSB-YP356853FFZ
CSB-EP356853FFZ
CSB-BP356853FFZ
CSB-MP356853FFZ
CSB-EP356853FFZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Modification methylase BsuRI (hsdRM) CSB-YP356854BRI
CSB-EP356854BRI
CSB-BP356854BRI
CSB-MP356854BRI
CSB-EP356854BRI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus amyloliquefaciens Degradation enzyme regulation protein DegQ (degQ) CSB-YP356855BQB
CSB-EP356855BQB
CSB-BP356855BQB
CSB-MP356855BQB
CSB-EP356855BQB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis HTH-type transcriptional regulator sinR (sinR) CSB-YP356856BRJ
CSB-EP356856BRJ
CSB-BP356856BRJ
CSB-MP356856BRJ
CSB-EP356856BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Sporulation initiation phosphotransferase B (spo0B) CSB-YP356857BRJ
CSB-EP356857BRJ
CSB-BP356857BRJ
CSB-MP356857BRJ
CSB-EP356857BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechococcus elongatus C-phycocyanin beta chain (cpcB1) CSB-YP356858FPY
CSB-EP356858FPY
CSB-BP356858FPY
CSB-MP356858FPY
CSB-EP356858FPY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Autographa californica nuclear polyhedrosis virus DNA-binding protein (P6.9) CSB-YP356859ARA
CSB-EP356859ARA
CSB-BP356859ARA
CSB-MP356859ARA
CSB-EP356859ARA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Beta-amylase, partial CSB-YP356860BAN
CSB-EP356860BAN
CSB-BP356860BAN
CSB-MP356860BAN
CSB-EP356860BAN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Protein lctB (lctB) CSB-YP356861BAJ
CSB-EP356861BAJ
CSB-BP356861BAJ
CSB-MP356861BAJ
CSB-EP356861BAJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacillus stearothermophilus Small, acid-soluble spore protein 1 (sasP-1) CSB-YP356862GFM
CSB-EP356862GFM
CSB-BP356862GFM
CSB-MP356862GFM
CSB-EP356862GFM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Penicillinase repressor (blaI) CSB-YP356863BQT
CSB-EP356863BQT
CSB-BP356863BQT
CSB-MP356863BQT
CSB-EP356863BQT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Corynebacterium glutamicum Anthranilate synthase component 1 (trpE), partial CSB-YP356864DWY
CSB-EP356864DWY
CSB-BP356864DWY
CSB-MP356864DWY
CSB-EP356864DWY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Corynebacterium glutamicum Anthranilate phosphoribosyltransferase (trpD) CSB-YP356865DWY
CSB-EP356865DWY
CSB-BP356865DWY
CSB-MP356865DWY
CSB-EP356865DWY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Corynebacterium glutamicum Tryptophan synthase beta chain (trpB) CSB-YP356866DWY
CSB-EP356866DWY
CSB-BP356866DWY
CSB-MP356866DWY
CSB-EP356866DWY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus sp. Endoglucanase, partial CSB-YP356867BBP
CSB-EP356867BBP
CSB-BP356867BBP
CSB-MP356867BBP
CSB-EP356867BBP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus sp. Endoglucanase A (celA) CSB-YP356868BCF
CSB-EP356868BCF
CSB-BP356868BCF
CSB-MP356868BCF
CSB-EP356868BCF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis TVP38/TMEM64 family membrane protein ytxB (ytxB), partial CSB-YP356869BRJ1
CSB-EP356869BRJ1
CSB-BP356869BRJ1
CSB-MP356869BRJ1
CSB-EP356869BRJ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Uncharacterized protein ytxC (ytxC) CSB-YP356870BRJ
CSB-EP356870BRJ
CSB-BP356870BRJ
CSB-MP356870BRJ
CSB-EP356870BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant rRNA adenine N-6-methyltransferase CSB-YP356871SNB
CSB-EP356871SNB
CSB-BP356871SNB
CSB-MP356871SNB
CSB-EP356871SNB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lysinibacillus sphaericus 41.9 kDa insecticidal toxin (binA) CSB-YP356872LRU
CSB-EP356872LRU
CSB-BP356872LRU
CSB-MP356872LRU
CSB-EP356872LRU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

Read More >>