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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Kanamycin nucleotidyltransferase (knt) CSB-YP356446FKZ
CSB-EP356446FKZ
CSB-BP356446FKZ
CSB-MP356446FKZ
CSB-EP356446FKZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Deoxyribodipyrimidine photo-lyase, mitochondrial (PHR1), partial CSB-YP356447SVG
CSB-EP356447SVG
CSB-BP356447SVG
CSB-MP356447SVG
CSB-EP356447SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Tobacco rattle virus Coat protein CSB-YP356449TFI
CSB-EP356449TFI
CSB-BP356449TFI
CSB-MP356449TFI
CSB-EP356449TFI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Tobacco rattle virus 29 kDa protein CSB-YP356450TFM
CSB-EP356450TFM
CSB-BP356450TFM
CSB-MP356450TFM
CSB-EP356450TFM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Tobacco rattle virus 16 kDa protein CSB-YP356451TFM
CSB-EP356451TFM
CSB-BP356451TFM
CSB-MP356451TFM
CSB-EP356451TFM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Tobacco rattle virus Protein 2b CSB-YP356452TFM
CSB-EP356452TFM
CSB-BP356452TFM
CSB-MP356452TFM
CSB-EP356452TFM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Tobacco rattle virus Replicase large subunit, partial CSB-YP356453TFL
CSB-EP356453TFL
CSB-BP356453TFL
CSB-MP356453TFL
CSB-EP356453TFL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Arginine metabolism regulation protein II (ARG81), partial CSB-YP356454SVG
CSB-EP356454SVG
CSB-BP356454SVG
CSB-MP356454SVG
CSB-EP356454SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Phaseolus vulgaris Erythroagglutinating phytohemagglutinin (DLEC1) CSB-YP356455EUN
CSB-EP356455EUN
CSB-BP356455EUN
CSB-MP356455EUN
CSB-EP356455EUN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dictyostelium discoideum Alpha-actinin A (abpA), partial CSB-YP356456DKK
CSB-EP356456DKK
CSB-BP356456DKK
CSB-MP356456DKK
CSB-EP356456DKK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Fremyella diplosiphon C-phycoerythrin beta chain (cpeB) CSB-YP356457FCY
CSB-EP356457FCY
CSB-BP356457FCY
CSB-MP356457FCY
CSB-EP356457FCY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Modification methylase EcoRII (ecoRIIM) CSB-YP356458ENL
CSB-EP356458ENL
CSB-BP356458ENL
CSB-MP356458ENL
CSB-EP356458ENL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Modification methylase PaeR7I (paeR7IM), partial CSB-YP356459EZW
CSB-EP356459EZW
CSB-BP356459EZW
CSB-MP356459EZW
CSB-EP356459EZW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Hyalophora cecropia Lysozyme CSB-YP356460HQQ
CSB-EP356460HQQ
CSB-BP356460HQQ
CSB-MP356460HQQ
CSB-EP356460HQQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Solanum lycopersicum Polygalacturonase-2 (PG2) CSB-YP356461SZY
CSB-EP356461SZY
CSB-BP356461SZY
CSB-MP356461SZY
CSB-EP356461SZY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Solanum lycopersicum Wound-induced proteinase inhibitor 2 CSB-YP356462SZY
CSB-EP356462SZY
CSB-BP356462SZY
CSB-MP356462SZY
CSB-EP356462SZY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila melanogaster Protein kinase C, brain isozyme (Pkc53E), partial CSB-YP356463DLU
CSB-EP356463DLU
CSB-BP356463DLU
CSB-MP356463DLU
CSB-EP356463DLU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Avian infectious bronchitis virus Spike glycoprotein (S), partial CSB-YP356464ARJ
CSB-EP356464ARJ
CSB-BP356464ARJ
CSB-MP356464ARJ
CSB-EP356464ARJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Avian infectious bronchitis virus Non-structural protein 3a (3a) CSB-YP356465ARW
CSB-EP356465ARW
CSB-BP356465ARW
CSB-MP356465ARW
CSB-EP356465ARW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Avian infectious bronchitis virus Envelope small membrane protein (E), partial CSB-YP356466ARW1
CSB-EP356466ARW1
CSB-BP356466ARW1
CSB-MP356466ARW1
CSB-EP356466ARW1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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