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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Insertion element iso-IS1N protein insA (insA) CSB-YP356154SZA
CSB-EP356154SZA
CSB-BP356154SZA
CSB-MP356154SZA
CSB-EP356154SZA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Insertion element iso-IS1n protein insB (insB) CSB-YP356155SZA
CSB-EP356155SZA
CSB-BP356155SZA
CSB-MP356155SZA
CSB-EP356155SZA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Maltose operon periplasmic protein (malM) CSB-YP356156ENV
CSB-EP356156ENV
CSB-BP356156ENV
CSB-MP356156ENV
CSB-EP356156ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Putative uncharacterized protein 1 CSB-YP356157ENL
CSB-EP356157ENL
CSB-BP356157ENL
CSB-MP356157ENL
CSB-EP356157ENL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Uncharacterized 9.4 kDa protein CSB-YP356158ENL
CSB-EP356158ENL
CSB-BP356158ENL
CSB-MP356158ENL
CSB-EP356158ENL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Uncharacterized 9.2 kDa protein CSB-YP356159ENL
CSB-EP356159ENL
CSB-BP356159ENL
CSB-MP356159ENL
CSB-EP356159ENL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Plasmid recombination enzyme type 1 (pre) CSB-YP356160FKZ
CSB-EP356160FKZ
CSB-BP356160FKZ
CSB-MP356160FKZ
CSB-EP356160FKZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Uncharacterized 12.4 kDa protein CSB-YP356161FKZ
CSB-EP356161FKZ
CSB-BP356161FKZ
CSB-MP356161FKZ
CSB-EP356161FKZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Protein rlx (rlx) CSB-YP356162FKZ
CSB-EP356162FKZ
CSB-BP356162FKZ
CSB-MP356162FKZ
CSB-EP356162FKZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Uncharacterized protein C CSB-YP356163FKZ
CSB-EP356163FKZ
CSB-BP356163FKZ
CSB-MP356163FKZ
CSB-EP356163FKZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Partitioning protein REP2 (REP2) CSB-YP356164SVG
CSB-EP356164SVG
CSB-BP356164SVG
CSB-MP356164SVG
CSB-EP356164SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Putative COX1/OXI3 intron 1 protein (aI1), partial CSB-YP356165SVG
CSB-EP356165SVG
CSB-BP356165SVG
CSB-MP356165SVG
CSB-EP356165SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Emericella nidulans Intron-encoded DNA endonuclease I-AniI (I-AniI), partial CSB-YP356166EBP
CSB-EP356166EBP
CSB-BP356166EBP
CSB-MP356166EBP
CSB-EP356166EBP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Transposable element Tc1 transposase (tc1a) CSB-YP356168CXY
CSB-EP356168CXY
CSB-BP356168CXY
CSB-MP356168CXY
CSB-EP356168CXY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Zea mays Uncharacterized protein ycf68 (ycf68) CSB-YP356169ZAX
CSB-EP356169ZAX
CSB-BP356169ZAX
CSB-MP356169ZAX
CSB-EP356169ZAX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Alicyclobacillus acidocaldarius Ferredoxin CSB-YP356170AHD
CSB-EP356170AHD
CSB-BP356170AHD
CSB-MP356170AHD
CSB-EP356170AHD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechococcus sp. C-phycocyanin subunit beta (cpcB) CSB-YP356171FQA
CSB-EP356171FQA
CSB-BP356171FQA
CSB-MP356171FQA
CSB-EP356171FQA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Anthranilate phosphoribosyltransferase (trpD) CSB-YP356172BRJ
CSB-EP356172BRJ
CSB-BP356172BRJ
CSB-MP356172BRJ
CSB-EP356172BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Macaca fuscata fuscata Pepsin A-1 (PGA) CSB-YP356173MMX
CSB-EP356173MMX
CSB-BP356173MMX
CSB-MP356173MMX
CSB-EP356173MMX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Potassium-transporting ATPase A chain (kdpA), partial CSB-YP356174ENV
CSB-EP356174ENV
CSB-BP356174ENV
CSB-MP356174ENV
CSB-EP356174ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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