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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Petunia sp. Chlorophyll a-b binding protein 91R, chloroplastic (CAB91R), partial CSB-YP356361PGN1
CSB-EP356361PGN1
CSB-BP356361PGN1
CSB-MP356361PGN1
CSB-EP356361PGN1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella typhimurium 3-isopropylmalate dehydratase small subunit 1 (leuD1) CSB-YP356362SXB
CSB-EP356362SXB
CSB-BP356362SXB
CSB-MP356362SXB
CSB-EP356362SXB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Glycine max 17.6 kDa class I heat shock protein (HSP17.6-L) CSB-YP356363GGV
CSB-EP356363GGV
CSB-BP356363GGV
CSB-MP356363GGV
CSB-EP356363GGV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Threonine--tRNA ligase, cytoplasmic (THS1), partial CSB-YP356364SVG
CSB-EP356364SVG
CSB-BP356364SVG
CSB-MP356364SVG
CSB-EP356364SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Tryptophan--tRNA ligase, mitochondrial (MSW1) CSB-YP356365SVG
CSB-EP356365SVG
CSB-BP356365SVG
CSB-MP356365SVG
CSB-EP356365SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila simulans Heat shock protein 83 (Hsp83) CSB-YP356366DMJ
CSB-EP356366DMJ
CSB-BP356366DMJ
CSB-MP356366DMJ
CSB-EP356366DMJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rat Seminal vesicle secretory protein 5 (Svs5) CSB-YP356367RA
CSB-EP356367RA
CSB-BP356367RA
CSB-MP356367RA
CSB-EP356367RA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila melanogaster Trypsin alpha (alphaTry) CSB-YP356368DLU
CSB-EP356368DLU
CSB-BP356368DLU
CSB-MP356368DLU
CSB-EP356368DLU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Leucine-specific-binding protein (livK) CSB-YP356369ENV
CSB-EP356369ENV
CSB-BP356369ENV
CSB-MP356369ENV
CSB-EP356369ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Cell division control protein 25 (CDC25), partial CSB-YP356370SVG
CSB-EP356370SVG
CSB-BP356370SVG
CSB-MP356370SVG
CSB-EP356370SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Paenibacillus macerans Cyclomaltodextrin glucanotransferase (cgtM), partial CSB-YP356372EQJ
CSB-EP356372EQJ
CSB-BP356372EQJ
CSB-MP356372EQJ
CSB-EP356372EQJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Small, acid-soluble spore protein B (sspB) CSB-YP356373BRJ
CSB-EP356373BRJ
CSB-BP356373BRJ
CSB-MP356373BRJ
CSB-EP356373BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Small, acid-soluble spore protein C4 (SASP-C4) CSB-YP356374BQV
CSB-EP356374BQV
CSB-BP356374BQV
CSB-MP356374BQV
CSB-EP356374BQV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Mitochondrial outer membrane protein porin 1 (POR1) CSB-YP356375SVG
CSB-EP356375SVG
CSB-BP356375SVG
CSB-MP356375SVG
CSB-EP356375SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pichia pastoris Alcohol oxidase 1 (AOX1) CSB-YP356376EVS
CSB-EP356376EVS
CSB-BP356376EVS
CSB-MP356376EVS
CSB-EP356376EVS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Lipoprotein 28 (nlpA) CSB-YP356377ENV
CSB-EP356377ENV
CSB-BP356377ENV
CSB-MP356377ENV
CSB-EP356377ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Simian virus 5 Hemagglutinin-neuraminidase (HN), partial CSB-YP356378SHU1
CSB-EP356378SHU1
CSB-BP356378SHU1
CSB-MP356378SHU1
CSB-EP356378SHU1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sendai virus Hemagglutinin-neuraminidase (HN), partial CSB-YP356379SFB1
CSB-EP356379SFB1
CSB-BP356379SFB1
CSB-MP356379SFB1
CSB-EP356379SFB1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sendai virus Fusion glycoprotein F0 (F), partial CSB-YP356380SEZ
CSB-EP356380SEZ
CSB-BP356380SEZ
CSB-MP356380SEZ
CSB-EP356380SEZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sendai virus Nucleoprotein (N), partial CSB-YP356381SFB
CSB-EP356381SFB
CSB-BP356381SFB
CSB-MP356381SFB
CSB-EP356381SFB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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