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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Major sperm protein 10/36/56/76 (msp-10) CSB-YP356572CXY
CSB-EP356572CXY
CSB-BP356572CXY
CSB-MP356572CXY
CSB-EP356572CXY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pisum sativum 30S ribosomal protein S14, chloroplastic (rps14) CSB-YP356573EWE
CSB-EP356573EWE
CSB-BP356573EWE
CSB-MP356573EWE
CSB-EP356573EWE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Zea mays Cytochrome b6 (petB), partial CSB-YP356574ZAX1
CSB-EP356574ZAX1
CSB-BP356574ZAX1
CSB-MP356574ZAX1
CSB-EP356574ZAX1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 3-isopropylmalate dehydrogenase (leuB) CSB-YP356575BAJ
CSB-EP356575BAJ
CSB-BP356575BAJ
CSB-MP356575BAJ
CSB-EP356575BAJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chaperone protein DnaK (dnaK), partial CSB-YP356576BQV
CSB-EP356576BQV
CSB-BP356576BQV
CSB-MP356576BQV
CSB-EP356576BQV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Chromosomal replication initiator protein DnaA (dnaA) CSB-YP356577BRJ
CSB-EP356577BRJ
CSB-BP356577BRJ
CSB-MP356577BRJ
CSB-EP356577BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Uncharacterized protein yaaA (yaaA) CSB-YP356578BRJ
CSB-EP356578BRJ
CSB-BP356578BRJ
CSB-MP356578BRJ
CSB-EP356578BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis DNA replication and repair protein recF (recF) CSB-YP356579BRJ
CSB-EP356579BRJ
CSB-BP356579BRJ
CSB-MP356579BRJ
CSB-EP356579BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Aspartate carbamoyltransferase (pyrB) CSB-YP356580BRJ
CSB-EP356580BRJ
CSB-BP356580BRJ
CSB-MP356580BRJ
CSB-EP356580BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Levanase (sacC), partial CSB-YP356581BRJ
CSB-BP356581BRJ
CSB-MP356581BRJ
CSB-EP356581BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acanthamoeba castellanii Myosin-2 heavy chain, non muscle, partial CSB-YP356582AAJ
CSB-EP356582AAJ
CSB-BP356582AAJ
CSB-MP356582AAJ
CSB-EP356582AAJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human adenovirus B serotype 7 Probable DNA-binding protein CSB-YP356583HII
CSB-EP356583HII
CSB-BP356583HII
CSB-MP356583HII
CSB-EP356583HII-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human adenovirus B serotype 7 Late L1 52 kDa protein CSB-YP356584HII
CSB-EP356584HII
CSB-BP356584HII
CSB-MP356584HII
CSB-EP356584HII-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human adenovirus B serotype 7 Uncharacterized 20.6 kDa early protein CSB-YP356585HII
CSB-EP356585HII
CSB-BP356585HII
CSB-MP356585HII
CSB-EP356585HII-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human adenovirus B serotype 7 Uncharacterized 14.5 kDa early protein CSB-YP356586HII
CSB-EP356586HII
CSB-BP356586HII
CSB-MP356586HII
CSB-EP356586HII-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human adenovirus B serotype 7 Uncharacterized 19.0 kDa early protein CSB-YP356587HII
CSB-EP356587HII
CSB-BP356587HII
CSB-MP356587HII
CSB-EP356587HII-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human adenovirus B serotype 7 Uncharacterized 11.3 kDa early protein CSB-YP356588HII
CSB-EP356588HII
CSB-BP356588HII
CSB-MP356588HII
CSB-EP356588HII-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Alfalfa mosaic virus Capsid protein (ORF3b) CSB-YP356589AHA
CSB-EP356589AHA
CSB-BP356589AHA
CSB-MP356589AHA
CSB-EP356589AHA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Venezuelan equine encephalitis virus Structural polyprotein, partial CSB-YP356590VAY1
CSB-EP356590VAY1
CSB-BP356590VAY1
CSB-MP356590VAY1
CSB-EP356590VAY1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechococcus sp. Uncharacterized serine protease syc0938_d (syc0938_d) CSB-YP356591FPZ
CSB-EP356591FPZ
CSB-BP356591FPZ
CSB-MP356591FPZ
CSB-EP356591FPZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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