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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant TrfA transcriptional repressor protein (trfA) CSB-YP357176ENL
CSB-EP357176ENL
CSB-BP357176ENL
CSB-MP357176ENL
CSB-EP357176ENL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhizopus oryzae Glucoamylase 1 CSB-YP357177RDE
CSB-EP357177RDE
CSB-BP357177RDE
CSB-MP357177RDE
CSB-EP357177RDE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neurospora crassa Histidine biosynthesis trifunctional protein (his-3), partial CSB-YP357178NHA
CSB-EP357178NHA
CSB-BP357178NHA
CSB-MP357178NHA
CSB-EP357178NHA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pisum sativum Ribulose bisphosphate carboxylase small chain 3A, chloroplastic (RBCS-3A) CSB-YP357179EWE
CSB-EP357179EWE
CSB-BP357179EWE
CSB-MP357179EWE
CSB-EP357179EWE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pisum sativum Glutamine synthetase root isozyme A (GS3A) CSB-YP357180EWE
CSB-EP357180EWE
CSB-BP357180EWE
CSB-MP357180EWE
CSB-EP357180EWE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Enterobacteria phage P2 Replication gene B protein (B) CSB-YP357181EDE
CSB-EP357181EDE
CSB-BP357181EDE
CSB-MP357181EDE
CSB-EP357181EDE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Potato virus X Coat protein CSB-YP357182PRC
CSB-EP357182PRC
CSB-BP357182PRC
CSB-MP357182PRC
CSB-EP357182PRC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila melanogaster Salivary glue protein Sgs-5 (Sgs5) CSB-YP357183DLU
CSB-EP357183DLU
CSB-BP357183DLU
CSB-MP357183DLU
CSB-EP357183DLU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila melanogaster Protein decapentaplegic (dpp) CSB-YP357184DLU
CSB-EP357184DLU
CSB-BP357184DLU
CSB-MP357184DLU
CSB-EP357184DLU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Enterobacteria phage T3 Gene 1.2 protein (1.2) CSB-YP357185EDY
CSB-EP357185EDY
CSB-BP357185EDY
CSB-MP357185EDY
CSB-EP357185EDY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Enterobacteria phage T3 Uncharacterized gene 1.6 protein (1.6) CSB-YP357186EDY
CSB-EP357186EDY
CSB-BP357186EDY
CSB-MP357186EDY
CSB-EP357186EDY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Tick-borne encephalitis virus Far Eastern subtype Genome polyprotein, partial CSB-YP357187TEN
CSB-MP357187TEN
CSB-EP357187TEN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. japonica Glutelin type-A 2 (GLUA2) CSB-YP357188OFG
CSB-EP357188OFG
CSB-BP357188OFG
CSB-MP357188OFG
CSB-EP357188OFG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis Gastrula-specific protein 17 (gs17) CSB-YP357189XBE
CSB-EP357189XBE
CSB-BP357189XBE
CSB-MP357189XBE
CSB-EP357189XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ustilago sphaerogena Guanyl-specific ribonuclease U1 CSB-YP357190UBD
CSB-EP357190UBD
CSB-BP357190UBD
CSB-MP357190UBD
CSB-EP357190UBD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio harveyi Alkanal monooxygenase alpha chain (luxA) CSB-YP357191VFD
CSB-EP357191VFD
CSB-BP357191VFD
CSB-MP357191VFD
CSB-EP357191VFD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oncorhynchus mykiss High mobility group-T protein CSB-YP357192OEI
CSB-EP357192OEI
CSB-BP357192OEI
CSB-MP357192OEI
CSB-EP357192OEI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nitrogen fixation protein fixZ (fixZ) CSB-YP357193RKN
CSB-EP357193RKN
CSB-BP357193RKN
CSB-MP357193RKN
CSB-EP357193RKN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Fructose-bisphosphate aldolase, glycosomal (ALD) CSB-YP357194TQV
CSB-EP357194TQV
CSB-BP357194TQV
CSB-MP357194TQV
CSB-EP357194TQV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse Alpha-1-antitrypsin 1-1 (Serpina1a) CSB-YP357195MO
CSB-EP357195MO
CSB-BP357195MO
CSB-MP357195MO
CSB-EP357195MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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