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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Escherichia coli Protein traJ (traJ) CSB-YP356893ENV
CSB-EP356893ENV
CSB-BP356893ENV
CSB-MP356893ENV
CSB-EP356893ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Sporulation initiation phosphotransferase F (spo0F) CSB-YP356894BRJ
CSB-EP356894BRJ
CSB-BP356894BRJ
CSB-MP356894BRJ
CSB-EP356894BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Uncharacterized protein eag (eag) CSB-YP356895BRJ
CSB-EP356895BRJ
CSB-BP356895BRJ
CSB-MP356895BRJ
CSB-EP356895BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Corynebacterium sp. 2,5-diketo-D-gluconic acid reductase A (dkgA) CSB-YP356896CQO
CSB-EP356896CQO
CSB-BP356896CQO
CSB-MP356896CQO
CSB-EP356896CQO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae ATP-dependent RNA helicase DED1 (DED1), partial CSB-YP356897SVG
CSB-EP356897SVG
CSB-BP356897SVG
CSB-MP356897SVG
CSB-EP356897SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis Hemoglobin subunit alpha-3 (hba3) CSB-YP356898XBE
CSB-EP356898XBE
CSB-BP356898XBE
CSB-MP356898XBE
CSB-EP356898XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis Hemoglobin subunit alpha-5 (hba5) CSB-YP356899XBE
CSB-EP356899XBE
CSB-BP356899XBE
CSB-MP356899XBE
CSB-EP356899XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bovine Hemoglobin subunit epsilon-4 (HBE4) CSB-YP356900BO
CSB-EP356900BO
CSB-BP356900BO
CSB-MP356900BO
CSB-EP356900BO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lachancea kluyveri Mating factor alpha CSB-YP356901LMA
CSB-EP356901LMA
CSB-BP356901LMA
CSB-MP356901LMA
CSB-EP356901LMA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus phage SPbeta Immunity protein (D) CSB-YP356902BQZ
CSB-EP356902BQZ
CSB-BP356902BQZ
CSB-MP356902BQZ
CSB-EP356902BQZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Schizosaccharomyces pombe M-phase inducer phosphatase (cdc25), partial CSB-YP356903SXV
CSB-EP356903SXV
CSB-BP356903SXV
CSB-MP356903SXV
CSB-EP356903SXV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus Immunoglobulin G-binding protein G (spg) CSB-YP356904SND
CSB-EP356904SND
CSB-BP356904SND
CSB-MP356904SND
CSB-EP356904SND-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Heat shock-like 85 kDa protein, partial CSB-YP356905TQY
CSB-EP356905TQY
CSB-BP356905TQY
CSB-MP356905TQY
CSB-EP356905TQY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thiobacillus ferrooxidans Nitrogenase molybdenum-iron protein alpha chain (nifD) CSB-YP356906TOA
CSB-EP356906TOA
CSB-BP356906TOA
CSB-MP356906TOA
CSB-EP356906TOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhizobium radiobacter Protein virC1 (virC1) CSB-YP356907RKV
CSB-EP356907RKV
CSB-BP356907RKV
CSB-MP356907RKV
CSB-EP356907RKV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhizobium radiobacter T-DNA border endonuclease virD1 (virD1) CSB-YP356908RKV
CSB-EP356908RKV
CSB-BP356908RKV
CSB-MP356908RKV
CSB-EP356908RKV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vicia faba Apocytochrome f (petA), partial CSB-YP356909VFJ1
CSB-EP356909VFJ1
CSB-BP356909VFJ1
CSB-MP356909VFJ1
CSB-EP356909VFJ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Zea mays Chlorophyll a-b binding protein, chloroplastic, partial CSB-YP356910ZAX1
CSB-EP356910ZAX1
CSB-BP356910ZAX1
CSB-MP356910ZAX1
CSB-EP356910ZAX1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Zea mays Zein-beta CSB-YP356911ZAX
CSB-EP356911ZAX
CSB-BP356911ZAX
CSB-MP356911ZAX
CSB-EP356911ZAX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Zea mays Zein-alpha 19B1 CSB-YP356912ZAX
CSB-EP356912ZAX
CSB-BP356912ZAX
CSB-MP356912ZAX
CSB-EP356912ZAX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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