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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Cyprinus carpio Prepro-urotensin II-gamma CSB-YP356873EQE
CSB-EP356873EQE
CSB-BP356873EQE
CSB-MP356873EQE
CSB-EP356873EQE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Heat shock protein Hsp-16.2 (hsp-16.2) CSB-YP356874CXY
CSB-EP356874CXY
CSB-BP356874CXY
CSB-MP356874CXY
CSB-EP356874CXY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pisum sativum Photosystem Q (B) protein, partial CSB-YP356875EWE1
CSB-EP356875EWE1
CSB-BP356875EWE1
CSB-MP356875EWE1
CSB-EP356875EWE1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pisum sativum 30S ribosomal protein S11, chloroplastic (rps11) CSB-YP356876EWE
CSB-EP356876EWE
CSB-BP356876EWE
CSB-MP356876EWE
CSB-EP356876EWE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Zea mays 50S ribosomal protein L22, chloroplastic (rpl22) CSB-YP356877ZAX
CSB-EP356877ZAX
CSB-BP356877ZAX
CSB-MP356877ZAX
CSB-EP356877ZAX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Murine coronavirus Envelope small membrane protein (E), partial CSB-YP356878MKA1
CSB-EP356878MKA1
CSB-BP356878MKA1
CSB-MP356878MKA1
CSB-EP356878MKA1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Avena sativa Phytochrome A type 3 (PHYA3), partial CSB-YP356879DPO
CSB-EP356879DPO
CSB-BP356879DPO
CSB-MP356879DPO
CSB-EP356879DPO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Avena sativa Phytochrome A type 5 (PHYA5) CSB-YP356880DPO
CSB-EP356880DPO
CSB-BP356880DPO
CSB-MP356880DPO
CSB-EP356880DPO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chlamydia trachomatis serovar L2 Major outer membrane porin (ompA) CSB-YP356881DSG
CSB-BP356881DSG
CSB-MP356881DSG
CSB-EP356881DSG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Daucus carota Extensin CSB-YP356882DIR
CSB-EP356882DIR
CSB-BP356882DIR
CSB-MP356882DIR
CSB-EP356882DIR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila melanogaster Segmentation protein paired (prd), partial CSB-YP356883DLU
CSB-EP356883DLU
CSB-BP356883DLU
CSB-MP356883DLU
CSB-EP356883DLU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila melanogaster Tubulin alpha-2 chain (alphaTub85E) CSB-YP356884DLU
CSB-EP356884DLU
CSB-BP356884DLU
CSB-MP356884DLU
CSB-EP356884DLU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila melanogaster Tubulin alpha-4 chain (alphaTub67C) CSB-YP356885DLU
CSB-EP356885DLU
CSB-BP356885DLU
CSB-MP356885DLU
CSB-EP356885DLU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila melanogaster Vitellogenin-3 (Yp3) CSB-YP356886DLU
CSB-EP356886DLU
CSB-BP356886DLU
CSB-MP356886DLU
CSB-EP356886DLU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Vitamin B12 import system permease protein BtuC (btuC), partial CSB-YP356887ENV
CSB-EP356887ENV
CSB-BP356887ENV
CSB-MP356887ENV
CSB-EP356887ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Resolvase (resD) CSB-YP356888ENV
CSB-EP356888ENV
CSB-BP356888ENV
CSB-MP356888ENV
CSB-EP356888ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Tryptophan 2-monooxygenase (iaaM), partial CSB-YP356889PWC
CSB-EP356889PWC
CSB-BP356889PWC
CSB-MP356889PWC
CSB-EP356889PWC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Indoleacetamide hydrolase (iaaH) CSB-YP356890PWC
CSB-EP356890PWC
CSB-BP356890PWC
CSB-MP356890PWC
CSB-EP356890PWC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas syringae pv. syringae Ice nucleation protein (inaZ), partial CSB-YP356891PWD
CSB-EP356891PWD
CSB-BP356891PWD
CSB-MP356891PWD
CSB-EP356891PWD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas putida Metapyrocatechase (xylE) CSB-YP356892FFZ
CSB-BP356892FFZ
CSB-MP356892FFZ
CSB-EP356892FFZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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