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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Human Dual specificity protein phosphatase 22 (DUSP22) CSB-EP865123HU 1mg(1mg*1 or 500ug*2)/100ug/20ug
Recombinant Human Three-prime repair exonuclease 1 (TREX1) CSB-BP865133HU 100μg/50μg/20μg/10μg
Recombinant Human DDB1- and CUL4-associated factor 16 (DCAF16) CSB-EP865168HU 1mg(1mg*1 or 500ug*2)/100μg/20μg
Recombinant Human Interleukin-37 (IL37) CSB-EP865190HU(A4) 1mg(1mg*1 or 500ug*2)/100ug/20ug
Recombinant Pisum sativum Zinc finger CCCH domain-containing protein ZFN-like CSB-EP866006EWE 1mg(1mg*1 or 500ug*2)/100μg/20μg
Recombinant Human Guanine nucleotide-binding protein subunit beta-like protein 1 (GNB1L) CSB-EP866315HU 1mg(1mg*1 or 500ug*2)/100μg/20μg
Recombinant Mouse Sclerostin domain-containing protein 1 (Sostdc1) CSB-BP866588MO 1mg(1mg*1 or 500ug*2)/100ug/20ug
Recombinant Human Phospholysine phosphohistidine inorganic pyrophosphate phosphatase (LHPP) CSB-EP867114HU 1mg(1mg*1 or 500ug*2)/100ug/20ug
Recombinant Human HAUS augmin-like complex subunit 4 (HAUS4) CSB-EP867157HU 1mg(1mg*1 or 500ug*2)/100μg/20μg
Recombinant Human AN1-type zinc finger protein 3 (ZFAND3) CSB-EP867174HU 1mg(1mg*1 or 500ug*2)/100ug/20ug
Recombinant Human Ankyrin repeat and SOCS box protein 6 (ASB6) CSB-EP868341HU 1mg(1mg*1 or 500ug*2)/100ug/20ug
Recombinant Human Thioredoxin-like protein 4B (TXNL4B) CSB-EP868342HU 1mg(1mg*1 or 500ug*2)/100ug/20ug
Recombinant Human Ganglioside-induced differentiation-associated protein 2 (GDAP2) CSB-EP868350HU 1mg(1mg*1 or 500ug*2)/100ug/20ug
Recombinant Human TGF-beta-activated kinase 1 and MAP3K7-binding protein 2 (TAB2), partial CSB-BP868358HU 1mg(1mg*1 or 500ug*2)/100ug/20ug
Recombinant Human Appetite-regulating hormone (GHRL) CSB-EP871388HU 1mg(1mg*1 or 500ug*2)/100ug/20ug
Recombinant Human CCR4-NOT transcription complex subunit 8 (CNOT8) CSB-EP871398HU 1mg(1mg*1 or 500ug*2)/100ug/20ug
Recombinant Human Programmed cell death protein 2-like (PDCD2L) CSB-EP871560HU 1mg(1mg*1 or 500ug*2)/100μg/20μg
Recombinant Human Nuclear distribution protein nudE-like 1 (NDEL1) CSB-EP872414HU 1mg(1mg*1 or 500ug*2)/100ug/20ug
Recombinant Human E3 ubiquitin-protein ligase SMURF2 (SMURF2) CSB-EP872532HU 1mg(1mg*1 or 500ug*2)/100ug/20ug
Recombinant Calloselasma rhodostoma Snaclec rhodocytin subunit beta,E.coli CSB-EP872766CBG 1mg(1mg*1 or 500ug*2)/100μg/20μg

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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