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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Mouse Protein Wnt-5a (Wnt5a) CSB-YP026138MO
CSB-BP026138MO
CSB-MP026138MO
CSB-EP026138MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Carbonic anhydrase 4 (CA4) CSB-YP004372HU
CSB-EP004372HU
CSB-BP004372HU
CSB-MP004372HU
CSB-EP004372HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rat Tumor necrosis factor receptor superfamily member 1A (Tnfrsf1a), partial CSB-YP023977RA1
CSB-EP023977RA1
CSB-BP023977RA1
CSB-MP023977RA1
CSB-EP023977RA1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Liver carboxylesterase 1 (CES1) CSB-YP005258HU
CSB-EP005258HU
CSB-EP005258HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Kell blood group glycoprotein (KEL), partial CSB-YP012148HU1
CSB-EP012148HU1
CSB-BP012148HU1
CSB-MP012148HU1
CSB-EP012148HU1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse Protein kinase C eta type (Prkch) CSB-YP018706MO
CSB-EP018706MO
CSB-BP018706MO
CSB-MP018706MO
CSB-EP018706MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bovine Ubiquitin carboxyl-terminal hydrolase isozyme L1 (UCHL1) CSB-YP025541BO
CSB-EP025541BO
CSB-BP025541BO
CSB-MP025541BO
CSB-EP025541BO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rat Brain-derived neurotrophic factor (Bdnf) CSB-YP002655RA
CSB-EP002655RA
CSB-BP002655RA
CSB-MP002655RA
CSB-EP002655RA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Glycine cleavage system H protein, mitochondrial (GCSH) CSB-YP009335HU
CSB-EP009335HU
CSB-BP009335HU
CSB-MP009335HU
CSB-EP009335HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rat Hepatocyte nuclear factor 3-alpha (Foxa1) CSB-YP008794RA
CSB-EP008794RA
CSB-BP008794RA
CSB-MP008794RA
CSB-EP008794RA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Adenosylhomocysteinase (AHCY) CSB-YP001474HU
CSB-BP001474HU
CSB-MP001474HU
CSB-EP001474HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bovine NADH dehydrogenase [ubiquinone] iron-sulfur protein 3, mitochondrial (NDUFS3) CSB-YP015662BO
CSB-EP015662BO
CSB-BP015662BO
CSB-MP015662BO
CSB-EP015662BO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Paired box protein Pax-7 (PAX7) CSB-YP017493HU
CSB-BP017493HU
CSB-MP017493HU
CSB-EP017493HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rat Sodium- and chloride-dependent GABA transporter 1 (Slc6a1), partial CSB-YP021687RA
CSB-EP021687RA
CSB-BP021687RA
CSB-MP021687RA
CSB-EP021687RA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rat Amphiregulin (Areg), partial CSB-YP001986RA1
CSB-EP001986RA1
CSB-BP001986RA1
CSB-MP001986RA1
CSB-EP001986RA1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dog Ras-related protein Rab-10 (RAB10) CSB-YP019152DO
CSB-EP019152DO
CSB-BP019152DO
CSB-MP019152DO
CSB-EP019152DO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Growth arrest and DNA damage-inducible protein GADD45 alpha (GADD45A) CSB-YP009161HU
CSB-BP009161HU
CSB-MP009161HU
CSB-EP009161HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human ATP synthase F (0) complex subunit B1, mitochondrial (ATP5PB) CSB-YP002358HU
CSB-BP002358HU
CSB-MP002358HU
CSB-EP002358HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bovine Insulin-like growth factor-binding protein 1 (IGFBP1) CSB-YP011095BO
CSB-EP011095BO
CSB-BP011095BO
CSB-MP011095BO
CSB-EP011095BO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse Paired box protein Pax-3 (Pax3) CSB-YP017489MO
CSB-EP017489MO
CSB-BP017489MO
CSB-MP017489MO
CSB-EP017489MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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