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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Anabaena sp. Flavodoxin (isiB) CSB-YP363383BYM
CSB-EP363383BYM
CSB-BP363383BYM
CSB-MP363383BYM
CSB-EP363383BYM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bordetella pertussis Ferric uptake regulation protein (fur) CSB-YP363384BUA
CSB-EP363384BUA
CSB-BP363384BUA
CSB-MP363384BUA
CSB-EP363384BUA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ferric uptake regulation protein (fur) CSB-YP363385BIK
CSB-EP363385BIK
CSB-BP363385BIK
CSB-MP363385BIK
CSB-EP363385BIK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Listeria innocua serovar 6a Transcriptional regulator ZurR (zurR) CSB-YP363386LGU
CSB-EP363386LGU
CSB-BP363386LGU
CSB-MP363386LGU
CSB-EP363386LGU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant tRNA uridine 5-carboxymethylaminomethyl modification enzyme MnmG (mnmG), partial CSB-YP363387SMH
CSB-EP363387SMH
CSB-BP363387SMH
CSB-MP363387SMH
CSB-EP363387SMH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Agrobacterium tumefaciens Glycogen synthase 1 (glgA1) CSB-YP363388AYS
CSB-EP363388AYS
CSB-BP363388AYS
CSB-MP363388AYS
CSB-EP363388AYS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechococcus elongatus Nitrogen regulatory protein P-II (glnB) CSB-YP363389FPY
CSB-EP363389FPY
CSB-BP363389FPY
CSB-MP363389FPY
CSB-EP363389FPY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Putative uncharacterized dsdX-like protein (dsdX) CSB-YP363390EOD
CSB-EP363390EOD
CSB-BP363390EOD
CSB-MP363390EOD
CSB-EP363390EOD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhizobium radiobacter Protein VirB11 (virB11) CSB-YP363391RKV
CSB-EP363391RKV
CSB-BP363391RKV
CSB-MP363391RKV
CSB-EP363391RKV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oscillatoria agardhii Gas vesicle structural protein (gvpA) CSB-YP363392OCP
CSB-EP363392OCP
CSB-BP363392OCP
CSB-MP363392OCP
CSB-EP363392OCP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Haloalkane dehalogenase (dhaA) CSB-YP363393RDH
CSB-EP363393RDH
CSB-BP363393RDH
CSB-MP363393RDH
CSB-EP363393RDH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Haloalkane dehalogenase (dhaA) CSB-YP363394PUR
CSB-EP363394PUR
CSB-BP363394PUR
CSB-MP363394PUR
CSB-EP363394PUR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactococcus lactis subsp. cremoris Lactococcin A secretion protein lcnD (lcnD), partial CSB-YP363395LNF
CSB-EP363395LNF
CSB-BP363395LNF
CSB-MP363395LNF
CSB-EP363395LNF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella suis biovar 1 Protein hfq (hfq) CSB-YP363396BMQ
CSB-EP363396BMQ
CSB-BP363396BMQ
CSB-MP363396BMQ
CSB-EP363396BMQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant DNA-binding protein HU (hup) CSB-YP363397BAI
CSB-EP363397BAI
CSB-BP363397BAI
CSB-MP363397BAI
CSB-EP363397BAI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant DNA-binding protein HRL18 CSB-YP363398RKN
CSB-EP363398RKN
CSB-BP363398RKN
CSB-MP363398RKN
CSB-EP363398RKN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptomyces coelicolor DNA-binding protein HU 1 (hup1) CSB-YP363399FOB
CSB-EP363399FOB
CSB-BP363399FOB
CSB-MP363399FOB
CSB-EP363399FOB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptomyces coelicolor DNA-binding protein HU 2 (hup2) CSB-YP363400FOB
CSB-EP363400FOB
CSB-BP363400FOB
CSB-MP363400FOB
CSB-EP363400FOB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhizobium radiobacter Integration host factor subunit beta (ihfB) CSB-YP363401RKV
CSB-EP363401RKV
CSB-BP363401RKV
CSB-MP363401RKV
CSB-EP363401RKV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Actinobacillus pleuropneumoniae RTX-II toxin-activating lysine-acyltransferase ApxIIC (apxIIC) CSB-YP363402AXG
CSB-EP363402AXG
CSB-BP363402AXG
CSB-MP363402AXG
CSB-EP363402AXG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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