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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Bordetella pertussis Cyclolysin-activating lysine-acyltransferase CyaC (cyaC) CSB-YP363403BUA
CSB-EP363403BUA
CSB-BP363403BUA
CSB-MP363403BUA
CSB-EP363403BUA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cyclolysin-activating lysine-acyltransferase CyaC (cyaC) CSB-YP363404BIK
CSB-EP363404BIK
CSB-BP363404BIK
CSB-MP363404BIK
CSB-EP363404BIK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhizobium radiobacter Heat-inducible transcription repressor HrcA (hrcA) CSB-YP363405RKV
CSB-EP363405RKV
CSB-BP363405RKV
CSB-MP363405RKV
CSB-EP363405RKV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactococcus lactis subsp. lactis Chaperone protein DnaK (dnaK), partial CSB-YP363406LNG
CSB-EP363406LNG
CSB-BP363406LNG
CSB-MP363406LNG
CSB-EP363406LNG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chaperone protein DnaK (dnaK), partial CSB-YP363407SMI
CSB-EP363407SMI
CSB-BP363407SMI
CSB-MP363407SMI
CSB-EP363407SMI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Transcriptional activator protein AhyR/AsaR (ahyR) CSB-YP363408AXW
CSB-EP363408AXW
CSB-BP363408AXW
CSB-MP363408AXW
CSB-EP363408AXW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhizobium radiobacter Uncharacterized protein 7 CSB-YP363409RKV
CSB-EP363409RKV
CSB-BP363409RKV
CSB-MP363409RKV
CSB-EP363409RKV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Agrobacterium tumefaciens Uncharacterized protein 7 CSB-YP363410AEZ
CSB-EP363410AEZ
CSB-BP363410AEZ
CSB-MP363410AEZ
CSB-EP363410AEZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Uncharacterized low temperature-induced protein all0457 homolog CSB-YP363411AJN
CSB-EP363411AJN
CSB-BP363411AJN
CSB-MP363411AJN
CSB-EP363411AJN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactococcus lactis subsp. cremoris Translation initiation factor IF-1 (infA) CSB-YP363413LNF
CSB-EP363413LNF
CSB-BP363413LNF
CSB-MP363413LNF
CSB-EP363413LNF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Agrobacterium tumefaciens Insertion element IS136 uncharacterized protein CSB-YP363414AFF
CSB-EP363414AFF
CSB-BP363414AFF
CSB-MP363414AFF
CSB-EP363414AFF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Agrobacterium tumefaciens Isopentenyl transferase (izt) CSB-YP363415AFF
CSB-EP363415AFF
CSB-BP363415AFF
CSB-MP363415AFF
CSB-EP363415AFF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pneumoniae Homoserine kinase (thrB) CSB-YP363416SMP
CSB-EP363416SMP
CSB-BP363416SMP
CSB-MP363416SMP
CSB-EP363416SMP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas aeruginosa Beta-lactamase SHV-5 (bla) CSB-YP363417EZW
CSB-EP363417EZW
CSB-BP363417EZW
CSB-MP363417EZW
CSB-EP363417EZW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Enterobacter aerogenes Beta-lactamase OXA-9 (bla) CSB-YP363418EKN
CSB-EP363418EKN
CSB-BP363418EKN
CSB-MP363418EKN
CSB-EP363418EKN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pneumoniae Penicillin-binding protein 2B (penA), partial CSB-YP363419SMP
CSB-EP363419SMP
CSB-BP363419SMP
CSB-MP363419SMP
CSB-EP363419SMP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactococcus lactis subsp. cremoris Lactococcin-A immunity protein (lciA) CSB-YP363420LNF
CSB-EP363420LNF
CSB-BP363420LNF
CSB-MP363420LNF
CSB-EP363420LNF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pneumoniae L-lactate dehydrogenase (ldh) CSB-YP363421SMP
CSB-EP363421SMP
CSB-BP363421SMP
CSB-MP363421SMP
CSB-EP363421SMP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Tetracycline resistance leader peptide (tetL) CSB-YP363422BQJ
CSB-EP363422BQJ
CSB-BP363422BQJ
CSB-MP363422BQJ
CSB-EP363422BQJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rickettsia conorii 17 kDa surface antigen (omp) CSB-YP363423RMS
CSB-EP363423RMS
CSB-BP363423RMS
CSB-MP363423RMS
CSB-EP363423RMS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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