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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Borreliella burgdorferi Outer surface protein A (ospA) CSB-BP363424BIO
CSB-MP363424BIO
CSB-EP363424BIO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella melitensis biotype 1 Outer membrane lipoprotein omp10 (omp10) CSB-YP363425BMO
CSB-EP363425BMO
CSB-BP363425BMO
CSB-MP363425BMO
CSB-EP363425BMO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella abortus biovar 1 Outer membrane lipoprotein omp10 (omp10) CSB-YP363426BWS
CSB-EP363426BWS
CSB-BP363426BWS
CSB-MP363426BWS
CSB-EP363426BWS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella suis biovar 1 Outer membrane lipoprotein omp19 (omp19) CSB-YP363427BMQ
CSB-EP363427BMQ
CSB-BP363427BMQ
CSB-MP363427BMQ
CSB-EP363427BMQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella suis biovar 1 UDP-3-O-acylglucosamine N-acyltransferase (lpxD) CSB-YP363428BMQ
CSB-EP363428BMQ
CSB-BP363428BMQ
CSB-MP363428BMQ
CSB-EP363428BMQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 4-alpha-glucanotransferase (malQ), partial CSB-YP363429FMW
CSB-EP363429FMW
CSB-BP363429FMW
CSB-MP363429FMW
CSB-EP363429FMW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella melitensis biotype 1 Methylglyoxal synthase (mgsA) CSB-YP363430BMO
CSB-EP363430BMO
CSB-BP363430BMO
CSB-MP363430BMO
CSB-EP363430BMO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Glycerol uptake facilitator protein (glpF), partial CSB-YP363431FMW1
CSB-EP363431FMW1
CSB-BP363431FMW1
CSB-MP363431FMW1
CSB-EP363431FMW1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Peptide methionine sulfoxide reductase MsrA/MsrB 1 (msrAB1) CSB-YP363432FMW
CSB-EP363432FMW
CSB-BP363432FMW
CSB-MP363432FMW
CSB-EP363432FMW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant DNA mismatch repair protein hexB (hexB), partial CSB-YP363433FMW
CSB-EP363433FMW
CSB-BP363433FMW
CSB-MP363433FMW
CSB-EP363433FMW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant DNA mismatch repair protein HexA (hexA), partial CSB-YP363434FMW
CSB-EP363434FMW
CSB-BP363434FMW
CSB-MP363434FMW
CSB-EP363434FMW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bordetella pertussis Pertussis toxin subunit 4 (ptxD) CSB-YP363435BUA
CSB-EP363435BUA
CSB-BP363435BUA
CSB-MP363435BUA
CSB-EP363435BUA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cell volume regulation protein A (cvrA), partial CSB-YP363436EGX
CSB-EP363436EGX
CSB-BP363436EGX
CSB-MP363436EGX
CSB-EP363436EGX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neocarzinostatin (ncsA) CSB-YP363437SNT
CSB-EP363437SNT
CSB-BP363437SNT
CSB-MP363437SNT
CSB-EP363437SNT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Anabaena variabilis Nitrogenase iron protein 1 (nifH1) CSB-YP363438BYN
CSB-EP363438BYN
CSB-BP363438BYN
CSB-MP363438BYN
CSB-EP363438BYN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant DNA-entry nuclease (endA), partial CSB-YP363439FMW
CSB-EP363439FMW
CSB-BP363439FMW
CSB-MP363439FMW
CSB-EP363439FMW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella melitensis biotype 1 Outer membrane lipoprotein Omp16 (omp16) CSB-YP363440BMO
CSB-EP363440BMO
CSB-BP363440BMO
CSB-MP363440BMO
CSB-EP363440BMO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella abortus biovar 1 Peptidoglycan-associated lipoprotein (pal) CSB-YP363441BWS
CSB-EP363441BWS
CSB-BP363441BWS
CSB-MP363441BWS
CSB-EP363441BWS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Agrobacterium tumefaciens Protein 6b (6b) CSB-YP363442AEZ
CSB-EP363442AEZ
CSB-BP363442AEZ
CSB-MP363442AEZ
CSB-EP363442AEZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella abortus biovar 1 31 kDa immunogenic protein (bcsP31) CSB-YP363443BWS
CSB-EP363443BWS
CSB-BP363443BWS
CSB-MP363443BWS
CSB-EP363443BWS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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