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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Thermosynechococcus elongatus Phosphoenolpyruvate carboxylase (ppc), partial CSB-YP363464TDQ
CSB-EP363464TDQ
CSB-BP363464TDQ
CSB-MP363464TDQ
CSB-EP363464TDQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Anabaena variabilis Cytochrome b6-f complex subunit 7 (petM), partial CSB-YP363465BYN
CSB-EP363465BYN
CSB-BP363465BYN
CSB-MP363465BYN
CSB-EP363465BYN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pneumoniae 2,3-bisphosphoglycerate-dependent phosphoglycerate mutase (gpmA) CSB-YP363466SMP
CSB-EP363466SMP
CSB-BP363466SMP
CSB-MP363466SMP
CSB-EP363466SMP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pneumoniae Phosphate transport system protein phoU (phoU) CSB-YP363468SMP
CSB-EP363468SMP
CSB-BP363468SMP
CSB-MP363468SMP
CSB-EP363468SMP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Putative peptidyl-prolyl cis-trans isomerase NifM (nifM) CSB-YP363469KBE
CSB-EP363469KBE
CSB-BP363469KBE
CSB-MP363469KBE
CSB-EP363469KBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella suis biovar 1 Probable periplasmic serine endoprotease DegP-like (htrA) CSB-YP363470BMQ
CSB-EP363470BMQ
CSB-BP363470BMQ
CSB-MP363470BMQ
CSB-EP363470BMQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Extracellular small neutral protease (snpA) CSB-YP363471FOI
CSB-EP363471FOI
CSB-BP363471FOI
CSB-MP363471FOI
CSB-EP363471FOI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactococcus lactis subsp. cremoris Pur operon repressor (purR) CSB-YP363472LND
CSB-EP363472LND
CSB-BP363472LND
CSB-MP363472LND
CSB-EP363472LND-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Photosystem I reaction center subunit III (psaF) CSB-YP363473SSD
CSB-EP363473SSD
CSB-BP363473SSD
CSB-MP363473SSD
CSB-EP363473SSD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Photosystem I reaction center subunit XII (psaM), partial CSB-YP363474SSD
CSB-EP363474SSD
CSB-BP363474SSD
CSB-MP363474SSD
CSB-EP363474SSD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Photosystem I P700 chlorophyll a apoprotein A1 (psaA), partial CSB-YP363475SSD
CSB-EP363475SSD
CSB-BP363475SSD
CSB-MP363475SSD
CSB-EP363475SSD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Photosystem I P700 chlorophyll a apoprotein A2 (psaB), partial CSB-YP363476SSD
CSB-EP363476SSD
CSB-BP363476SSD
CSB-MP363476SSD
CSB-EP363476SSD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nostoc sp. Photosystem I iron-sulfur center (psaC) CSB-YP363477NHR
CSB-EP363477NHR
CSB-BP363477NHR
CSB-MP363477NHR
CSB-EP363477NHR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Fremyella diplosiphon Photosystem I iron-sulfur center (psaC) CSB-YP363478FCY
CSB-EP363478FCY
CSB-BP363478FCY
CSB-MP363478FCY
CSB-EP363478FCY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nostoc sp. Photosystem I iron-sulfur center (psaC) CSB-YP363479NHS
CSB-EP363479NHS
CSB-BP363479NHS
CSB-MP363479NHS
CSB-EP363479NHS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Photosystem I iron-sulfur center (psaC) CSB-YP363480SSD
CSB-EP363480SSD
CSB-BP363480SSD
CSB-MP363480SSD
CSB-EP363480SSD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechococcus sp. Photosystem I iron-sulfur center (psaC) CSB-YP363481SSL
CSB-EP363481SSL
CSB-BP363481SSL
CSB-MP363481SSL
CSB-EP363481SSL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermosynechococcus elongatus Photosystem I reaction center subunit II (psaD) CSB-YP363482TDQ
CSB-EP363482TDQ
CSB-BP363482TDQ
CSB-MP363482TDQ
CSB-EP363482TDQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermosynechococcus vulcanus Photosystem I reaction center subunit II (psaD) CSB-YP363483TNI
CSB-EP363483TNI
CSB-BP363483TNI
CSB-MP363483TNI
CSB-EP363483TNI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Photosystem I reaction center subunit IV (psaE) CSB-YP363484SSD
CSB-EP363484SSD
CSB-BP363484SSD
CSB-MP363484SSD
CSB-EP363484SSD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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