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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Rabbit Elongation factor 1-alpha 1 (EEF1A1) CSB-YP007409RB
CSB-EP007409RB
CSB-BP007409RB
CSB-MP007409RB
CSB-EP007409RB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse Actin, alpha skeletal muscle (Acta1) CSB-YP001205MO
CSB-EP001205MO
CSB-BP001205MO
CSB-MP001205MO
CSB-EP001205MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chicken Actin, alpha skeletal muscle (ACTA1) CSB-YP001205CH
CSB-EP001205CH
CSB-BP001205CH
CSB-MP001205CH
CSB-EP001205CH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sheep Fibrinogen alpha chain (FGA) CSB-YP008607SH
CSB-EP008607SH
CSB-BP008607SH
CSB-MP008607SH
CSB-EP008607SH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bovine 14-3-3 protein beta/alpha (YWHAB) CSB-YP026285BO
CSB-EP026285BO
CSB-BP026285BO
CSB-MP026285BO
CSB-EP026285BO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse Tubulin alpha-4A chain (Tuba4a) CSB-YP025313MO
CSB-BP025313MO
CSB-MP025313MO
CSB-EP025313MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse Protein kinase C beta type (Prkcb) CSB-YP018700MO
CSB-EP018700MO
CSB-BP018700MO
CSB-MP018700MO
CSB-EP018700MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rat Endothelial differentiation-related factor 1 (Edf1) CSB-YP007398RA
CSB-EP007398RA
CSB-BP007398RA
CSB-MP007398RA
CSB-EP007398RA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Hemoglobin subunit gamma-2 (HBG2) CSB-YP010156HU
CSB-BP010156HU
CSB-MP010156HU
CSB-EP010156HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse Zinc fingers and homeoboxes protein 1 (Zhx1) CSB-YP026481MO
CSB-EP026481MO
CSB-BP026481MO
CSB-MP026481MO
CSB-EP026481MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse Double C2-like domain-containing protein beta (Doc2b) CSB-YP007092MO
CSB-EP007092MO
CSB-BP007092MO
CSB-MP007092MO
CSB-EP007092MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse Phosphatidylinositol 4-phosphate 5-kinase type-1 alpha (Pip5k1a) CSB-YP018025MO
CSB-EP018025MO
CSB-BP018025MO
CSB-MP018025MO
CSB-EP018025MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse C-type lectin domain family 4 member F (Clec4f), partial CSB-YP005536MO1
CSB-EP005536MO1
CSB-BP005536MO1
CSB-MP005536MO1
CSB-EP005536MO1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse Plexin-A3 (Plxna3), partial CSB-YP018219MO
CSB-EP018219MO
CSB-BP018219MO
CSB-MP018219MO
CSB-EP018219MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse GTPase IMAP family member 1 (Gimap1), partial CSB-YP009420MO1
CSB-EP009420MO1
CSB-BP009420MO1
CSB-MP009420MO1
CSB-EP009420MO1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse Nuclear factor 1 C-type (Nfic) CSB-YP015756MO
CSB-EP015756MO
CSB-BP015756MO
CSB-MP015756MO
CSB-EP015756MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse Cathepsin E (Ctse) CSB-YP006188MO
CSB-EP006188MO
CSB-BP006188MO
CSB-MP006188MO
CSB-EP006188MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse Stimulated by retinoic acid gene 8 protein (Stra8) CSB-YP022873MO
CSB-EP022873MO
CSB-BP022873MO
CSB-MP022873MO
CSB-EP022873MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse Receptor-type tyrosine-protein phosphatase V (Ptprv), partial CSB-YP019067MO
CSB-EP019067MO
CSB-BP019067MO
CSB-MP019067MO
CSB-EP019067MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse Nitric oxide synthase, endothelial (Nos3), partial CSB-YP015946MO
CSB-EP015946MO
CSB-BP015946MO
CSB-MP015946MO
CSB-EP015946MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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