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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Neosartorya fischeri ATP-dependent RNA helicase dbp10 (dbp10), partial CSB-YP374068NEZ
CSB-EP374068NEZ
CSB-BP374068NEZ
CSB-MP374068NEZ
CSB-EP374068NEZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neosartorya fischeri Probable beta-glucosidase J (bglJ), partial CSB-YP374069NEZ
CSB-EP374069NEZ
CSB-BP374069NEZ
CSB-MP374069NEZ
CSB-EP374069NEZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neosartorya fischeri WD repeat-containing protein NFIA_058290 (NFIA_058290), partial CSB-YP374070NEZ
CSB-EP374070NEZ
CSB-BP374070NEZ
CSB-MP374070NEZ
CSB-EP374070NEZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neosartorya fischeri Protein transport protein SEC24 (sec24), partial CSB-YP374071NEZ
CSB-EP374071NEZ
CSB-BP374071NEZ
CSB-MP374071NEZ
CSB-EP374071NEZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neosartorya fischeri Autophagy-related protein 2 (atg2), partial CSB-YP374072NEZ
CSB-EP374072NEZ
CSB-BP374072NEZ
CSB-MP374072NEZ
CSB-EP374072NEZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neosartorya fischeri U3 small nucleolar RNA-associated protein 10 (utp10), partial CSB-YP374073NEZ
CSB-EP374073NEZ
CSB-BP374073NEZ
CSB-MP374073NEZ
CSB-EP374073NEZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neosartorya fischeri Carboxypeptidase Y homolog A (cpyA) CSB-YP374074NEZ
CSB-EP374074NEZ
CSB-BP374074NEZ
CSB-MP374074NEZ
CSB-EP374074NEZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neosartorya fischeri Probable pectate lyase C (plyC) CSB-YP374075NEZ
CSB-EP374075NEZ
CSB-BP374075NEZ
CSB-MP374075NEZ
CSB-EP374075NEZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Halobacillus halophilus Uncharacterized methyltransferase YrrT CSB-YP374076HSF
CSB-EP374076HSF
CSB-BP374076HSF
CSB-MP374076HSF
CSB-EP374076HSF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chrysanthemum morifolium Chalcone--flavonone isomerase 2 (CHI2) CSB-YP374077CWQ
CSB-EP374077CWQ
CSB-BP374077CWQ
CSB-MP374077CWQ
CSB-EP374077CWQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Hordeum vulgare 30S ribosomal protein S19, chloroplastic (rps19) CSB-YP374078HWQ
CSB-EP374078HWQ
CSB-BP374078HWQ
CSB-MP374078HWQ
CSB-EP374078HWQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Hordeum vulgare DNA-directed RNA polymerase subunit beta (rpoB), partial CSB-YP374079HWQ
CSB-EP374079HWQ
CSB-BP374079HWQ
CSB-MP374079HWQ
CSB-EP374079HWQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Hordeum vulgare DNA-directed RNA polymerase subunit beta' (rpoC2), partial CSB-YP374080HWQ
CSB-EP374080HWQ
CSB-BP374080HWQ
CSB-MP374080HWQ
CSB-EP374080HWQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Hordeum vulgare ATP synthase subunit a, chloroplastic (atpI), partial CSB-YP374081HWQ1
CSB-EP374081HWQ1
CSB-BP374081HWQ1
CSB-MP374081HWQ1
CSB-EP374081HWQ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Hordeum vulgare ATP synthase subunit c, chloroplastic (atpH), partial CSB-YP374082HWQ1
CSB-EP374082HWQ1
CSB-BP374082HWQ1
CSB-MP374082HWQ1
CSB-EP374082HWQ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Hordeum vulgare ATP synthase subunit alpha, chloroplastic (atpA), partial CSB-YP374083HWQ
CSB-EP374083HWQ
CSB-BP374083HWQ
CSB-MP374083HWQ
CSB-EP374083HWQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Hordeum vulgare Photosystem I P700 chlorophyll a apoprotein A2 (psaB), partial CSB-YP374084HWQ
CSB-EP374084HWQ
CSB-BP374084HWQ
CSB-MP374084HWQ
CSB-EP374084HWQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Hordeum vulgare Photosystem I assembly protein ycf3 (ycf3) CSB-YP374085HWQ
CSB-EP374085HWQ
CSB-BP374085HWQ
CSB-MP374085HWQ
CSB-EP374085HWQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Hordeum vulgare NAD (P)H-quinone oxidoreductase subunit 3, chloroplastic, partial CSB-YP374086HWQ1
CSB-EP374086HWQ1
CSB-BP374086HWQ1
CSB-MP374086HWQ1
CSB-EP374086HWQ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Hordeum vulgare Apocytochrome f (petA), partial CSB-YP374087HWQ1
CSB-EP374087HWQ1
CSB-BP374087HWQ1
CSB-MP374087HWQ1
CSB-EP374087HWQ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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