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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Neisseria meningitidis serogroup C / serotype 2a NADH-quinone oxidoreductase subunit K (nuoK), partial CSB-YP375869NEX1
CSB-EP375869NEX1
CSB-BP375869NEX1
CSB-MP375869NEX1
CSB-EP375869NEX1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neisseria meningitidis serogroup C / serotype 2a Indole-3-glycerol phosphate synthase (trpC) CSB-YP375870NEX
CSB-EP375870NEX
CSB-BP375870NEX
CSB-MP375870NEX
CSB-EP375870NEX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neisseria meningitidis serogroup C / serotype 2a Ribonuclease P protein component (rnpA) CSB-YP375871NEX
CSB-EP375871NEX
CSB-BP375871NEX
CSB-MP375871NEX
CSB-EP375871NEX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neisseria meningitidis serogroup C / serotype 2a Chromosomal replication initiator protein DnaA (dnaA), partial CSB-YP375872NEX
CSB-EP375872NEX
CSB-BP375872NEX
CSB-MP375872NEX
CSB-EP375872NEX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neisseria meningitidis serogroup C / serotype 2a Formate--tetrahydrofolate ligase (fhs), partial CSB-YP375873NEX
CSB-EP375873NEX
CSB-BP375873NEX
CSB-MP375873NEX
CSB-EP375873NEX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neisseria meningitidis serogroup C / serotype 2a Tyrosine--tRNA ligase (tyrS) CSB-YP375874NEX
CSB-EP375874NEX
CSB-BP375874NEX
CSB-MP375874NEX
CSB-EP375874NEX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neisseria meningitidis serogroup C / serotype 2a 4-hydroxy-3-methylbut-2-enyl diphosphate reductase (ispH) CSB-YP375875NEX
CSB-EP375875NEX
CSB-BP375875NEX
CSB-MP375875NEX
CSB-EP375875NEX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neisseria meningitidis serogroup C / serotype 2a Protein-export protein secB (secB) CSB-YP375876NEX
CSB-EP375876NEX
CSB-BP375876NEX
CSB-MP375876NEX
CSB-EP375876NEX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neisseria meningitidis serogroup C / serotype 2a UDP-2,3-diacylglucosamine hydrolase (lpxH) CSB-YP375877NEX
CSB-EP375877NEX
CSB-BP375877NEX
CSB-MP375877NEX
CSB-EP375877NEX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neisseria meningitidis serogroup C / serotype 2a Na (+)-translocating NADH-quinone reductase subunit E, partial CSB-YP375878NEX1
CSB-EP375878NEX1
CSB-BP375878NEX1
CSB-MP375878NEX1
CSB-EP375878NEX1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neisseria meningitidis serogroup C / serotype 2a Glutamyl-tRNA reductase (hemA) CSB-YP375879NEX
CSB-EP375879NEX
CSB-BP375879NEX
CSB-MP375879NEX
CSB-EP375879NEX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neisseria meningitidis serogroup C / serotype 2a Sec-independent protein translocase protein TatB (tatB), partial CSB-YP375880NEX
CSB-EP375880NEX
CSB-BP375880NEX
CSB-MP375880NEX
CSB-EP375880NEX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neisseria meningitidis serogroup C / serotype 2a Peptide chain release factor 3 (prfC), partial CSB-YP375881NEX
CSB-EP375881NEX
CSB-BP375881NEX
CSB-MP375881NEX
CSB-EP375881NEX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neisseria meningitidis serogroup C / serotype 2a Phosphoribosyl-AMP cyclohydrolase (hisI) CSB-YP375882NEX
CSB-EP375882NEX
CSB-BP375882NEX
CSB-MP375882NEX
CSB-EP375882NEX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neisseria meningitidis serogroup C / serotype 2a DNA ligase (ligA), partial CSB-YP375883NEX
CSB-EP375883NEX
CSB-BP375883NEX
CSB-MP375883NEX
CSB-EP375883NEX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neisseria meningitidis serogroup C / serotype 2a UPF0434 protein NMC0623 (NMC0623) CSB-YP375884NEX
CSB-EP375884NEX
CSB-BP375884NEX
CSB-MP375884NEX
CSB-EP375884NEX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neisseria meningitidis serogroup C / serotype 2a 6,7-dimethyl-8-ribityllumazine synthase (ribH) CSB-YP375885NEX
CSB-EP375885NEX
CSB-BP375885NEX
CSB-MP375885NEX
CSB-EP375885NEX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neisseria meningitidis serogroup C / serotype 2a Ribosomal RNA small subunit methyltransferase A (rsmA) CSB-YP375886NEX
CSB-EP375886NEX
CSB-BP375886NEX
CSB-MP375886NEX
CSB-EP375886NEX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neisseria meningitidis serogroup C / serotype 2a HPr kinase/phosphorylase (hprK) CSB-YP375887NEX
CSB-EP375887NEX
CSB-BP375887NEX
CSB-MP375887NEX
CSB-EP375887NEX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neisseria meningitidis serogroup C / serotype 2a UPF0042 nucleotide-binding protein NMC0691 (NMC0691) CSB-YP375888NEX
CSB-EP375888NEX
CSB-BP375888NEX
CSB-MP375888NEX
CSB-EP375888NEX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務,您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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