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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Neisseria meningitidis serogroup C / serotype 2a 3-isopropylmalate dehydratase small subunit (leuD) CSB-YP375469NEX
CSB-EP375469NEX
CSB-BP375469NEX
CSB-MP375469NEX
CSB-EP375469NEX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neisseria meningitidis serogroup C / serotype 2a Aspartate 1-decarboxylase (panD) CSB-YP375470NEX
CSB-EP375470NEX
CSB-BP375470NEX
CSB-MP375470NEX
CSB-EP375470NEX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neisseria meningitidis serogroup C / serotype 2a Integration host factor subunit beta (ihfB) CSB-YP375471NEX
CSB-EP375471NEX
CSB-BP375471NEX
CSB-MP375471NEX
CSB-EP375471NEX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neisseria meningitidis serogroup C / serotype 2a ATP-dependent Clp protease proteolytic subunit (clpP) CSB-YP375472NEX
CSB-EP375472NEX
CSB-BP375472NEX
CSB-MP375472NEX
CSB-EP375472NEX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neisseria meningitidis serogroup C / serotype 2a Primosomal replication protein n (priB) CSB-YP375473NEX
CSB-EP375473NEX
CSB-BP375473NEX
CSB-MP375473NEX
CSB-EP375473NEX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neisseria meningitidis serogroup C / serotype 2a tRNA (guanine-N (7)-)-methyltransferase CSB-YP375474NEX
CSB-EP375474NEX
CSB-BP375474NEX
CSB-MP375474NEX
CSB-EP375474NEX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neisseria meningitidis serogroup C / serotype 2a Proline--tRNA ligase (proS), partial CSB-YP375475NEX
CSB-EP375475NEX
CSB-BP375475NEX
CSB-MP375475NEX
CSB-EP375475NEX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neisseria meningitidis serogroup C / serotype 2a Glutamyl-tRNA (Gln) amidotransferase subunit A CSB-YP375476NEX
CSB-EP375476NEX
CSB-BP375476NEX
CSB-MP375476NEX
CSB-EP375476NEX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neisseria meningitidis serogroup C / serotype 2a Co-chaperone protein hscB homolog (hscB) CSB-YP375477NEX
CSB-EP375477NEX
CSB-BP375477NEX
CSB-MP375477NEX
CSB-EP375477NEX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neisseria meningitidis serogroup C / serotype 2a Crossover junction endodeoxyribonuclease RuvC (ruvC) CSB-YP375478NEX
CSB-EP375478NEX
CSB-BP375478NEX
CSB-MP375478NEX
CSB-EP375478NEX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neisseria meningitidis serogroup C / serotype 2a Regulatory protein recX (recX) CSB-YP375479NEX
CSB-EP375479NEX
CSB-BP375479NEX
CSB-MP375479NEX
CSB-EP375479NEX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neisseria meningitidis serogroup C / serotype 2a 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase (ispF) CSB-YP375480NEX
CSB-EP375480NEX
CSB-BP375480NEX
CSB-MP375480NEX
CSB-EP375480NEX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neisseria meningitidis serogroup C / serotype 2a ATP phosphoribosyltransferase (hisG) CSB-YP375481NEX
CSB-EP375481NEX
CSB-BP375481NEX
CSB-MP375481NEX
CSB-EP375481NEX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neisseria meningitidis serogroup C / serotype 2a Alanine--tRNA ligase (alaS), partial CSB-YP375482NEX
CSB-EP375482NEX
CSB-BP375482NEX
CSB-MP375482NEX
CSB-EP375482NEX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neisseria meningitidis serogroup C / serotype 2a Ribosome maturation factor RimP (rimP) CSB-YP375483NEX
CSB-EP375483NEX
CSB-BP375483NEX
CSB-MP375483NEX
CSB-EP375483NEX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neisseria meningitidis serogroup C / serotype 2a RNA pyrophosphohydrolase (rppH) CSB-YP375484NEX
CSB-EP375484NEX
CSB-BP375484NEX
CSB-MP375484NEX
CSB-EP375484NEX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neisseria meningitidis serogroup C / serotype 2a Phosphoglucosamine mutase (glmM) CSB-YP375485NEX
CSB-EP375485NEX
CSB-BP375485NEX
CSB-MP375485NEX
CSB-EP375485NEX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neisseria meningitidis serogroup C / serotype 2a Putative 8-amino-7-oxononanoate synthase (bioF) CSB-YP375486NEX
CSB-EP375486NEX
CSB-BP375486NEX
CSB-MP375486NEX
CSB-EP375486NEX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neisseria meningitidis serogroup C / serotype 2a Aspartate--tRNA ligase (aspS), partial CSB-YP375487NEX
CSB-EP375487NEX
CSB-BP375487NEX
CSB-MP375487NEX
CSB-EP375487NEX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neisseria meningitidis serogroup C / serotype 2a DNA repair protein recO (recO) CSB-YP375488NEX
CSB-EP375488NEX
CSB-BP375488NEX
CSB-MP375488NEX
CSB-EP375488NEX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標題所示,本文主要總結了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務,您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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