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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Nocardioides sp. NADH-quinone oxidoreductase subunit K (nuoK), partial CSB-YP376469NFC1
CSB-EP376469NFC1
CSB-BP376469NFC1
CSB-MP376469NFC1
CSB-EP376469NFC1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nocardioides sp. UPF0234 protein Noca_0564 (Noca_0564) CSB-YP376470NFC
CSB-EP376470NFC
CSB-BP376470NFC
CSB-MP376470NFC
CSB-EP376470NFC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nocardioides sp. 50S ribosomal protein L1 (rplA) CSB-YP376471NFC
CSB-EP376471NFC
CSB-BP376471NFC
CSB-MP376471NFC
CSB-EP376471NFC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nocardioides sp. DNA-directed RNA polymerase subunit beta' (rpoC), partial CSB-YP376472NFC
CSB-EP376472NFC
CSB-BP376472NFC
CSB-MP376472NFC
CSB-EP376472NFC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nocardioides sp. Flagellar hook-basal body complex protein FliE (fliE) CSB-YP376473NFC
CSB-EP376473NFC
CSB-BP376473NFC
CSB-MP376473NFC
CSB-EP376473NFC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nocardioides sp. Molybdenum cofactor biosynthesis protein C (moaC) CSB-YP376474NFC
CSB-EP376474NFC
CSB-BP376474NFC
CSB-MP376474NFC
CSB-EP376474NFC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nocardioides sp. Phosphoglucosamine mutase (glmM) CSB-YP376475NFC
CSB-EP376475NFC
CSB-BP376475NFC
CSB-MP376475NFC
CSB-EP376475NFC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nocardioides sp. Enolase (eno) CSB-YP376476NFC
CSB-EP376476NFC
CSB-BP376476NFC
CSB-MP376476NFC
CSB-EP376476NFC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nocardioides sp. Methionine--tRNA ligase (metG), partial CSB-YP376477NFC
CSB-EP376477NFC
CSB-BP376477NFC
CSB-MP376477NFC
CSB-EP376477NFC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nocardioides sp. 4-hydroxy-2-oxovalerate aldolase 1 (Noca_1649) CSB-YP376478NFC
CSB-EP376478NFC
CSB-BP376478NFC
CSB-MP376478NFC
CSB-EP376478NFC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nocardioides sp. ATP synthase subunit c (atpE), partial CSB-YP376479NFC1
CSB-EP376479NFC1
CSB-BP376479NFC1
CSB-MP376479NFC1
CSB-EP376479NFC1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nocardioides sp. Gamma-glutamyl phosphate reductase (proA) CSB-YP376480NFC
CSB-EP376480NFC
CSB-BP376480NFC
CSB-MP376480NFC
CSB-EP376480NFC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nocardioides sp. 30S ribosomal protein S20 (rpsT) CSB-YP376481NFC
CSB-EP376481NFC
CSB-BP376481NFC
CSB-MP376481NFC
CSB-EP376481NFC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nocardioides sp. 3-methyl-2-oxobutanoate hydroxymethyltransferase (panB) CSB-YP376482NFC
CSB-EP376482NFC
CSB-BP376482NFC
CSB-MP376482NFC
CSB-EP376482NFC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nocardioides sp. Threonine--tRNA ligase (thrS), partial CSB-YP376483NFC
CSB-EP376483NFC
CSB-BP376483NFC
CSB-MP376483NFC
CSB-EP376483NFC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nocardioides sp. Crossover junction endodeoxyribonuclease RuvC (ruvC) CSB-YP376484NFC
CSB-EP376484NFC
CSB-BP376484NFC
CSB-MP376484NFC
CSB-EP376484NFC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nocardioides sp. Alanine--tRNA ligase (alaS), partial CSB-YP376485NFC
CSB-EP376485NFC
CSB-BP376485NFC
CSB-MP376485NFC
CSB-EP376485NFC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nocardioides sp. Elongation factor P (efp) CSB-YP376486NFC
CSB-EP376486NFC
CSB-BP376486NFC
CSB-MP376486NFC
CSB-EP376486NFC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nocardioides sp. Aspartate carbamoyltransferase (pyrB) CSB-YP376487NFC
CSB-EP376487NFC
CSB-BP376487NFC
CSB-MP376487NFC
CSB-EP376487NFC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nocardioides sp. S-adenosylmethionine synthase (metK) CSB-YP376488NFC
CSB-EP376488NFC
CSB-BP376488NFC
CSB-MP376488NFC
CSB-EP376488NFC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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