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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Agrostis stolonifera Photosystem II reaction center protein H (psbH), partial CSB-YP375509AUM1
CSB-EP375509AUM1
CSB-BP375509AUM1
CSB-MP375509AUM1
CSB-EP375509AUM1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Agrostis stolonifera Translation initiation factor IF-1, chloroplastic (infA) CSB-YP375510AUM
CSB-EP375510AUM
CSB-BP375510AUM
CSB-MP375510AUM
CSB-EP375510AUM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Agrostis stolonifera 50S ribosomal protein L22, chloroplastic (rpl22) CSB-YP375511AUM
CSB-EP375511AUM
CSB-BP375511AUM
CSB-MP375511AUM
CSB-EP375511AUM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Agrostis stolonifera 30S ribosomal protein S7, chloroplastic (rps7-A) CSB-YP375512AUM
CSB-EP375512AUM
CSB-BP375512AUM
CSB-MP375512AUM
CSB-EP375512AUM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Agrostis stolonifera 50S ribosomal protein L32, chloroplastic (rpl32) CSB-YP375513AUM
CSB-EP375513AUM
CSB-BP375513AUM
CSB-MP375513AUM
CSB-EP375513AUM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Agrostis stolonifera NAD (P)H-quinone oxidoreductase subunit 6, chloroplastic, partial CSB-YP375514AUM1
CSB-EP375514AUM1
CSB-BP375514AUM1
CSB-MP375514AUM1
CSB-EP375514AUM1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Zinnia elegans CLAVATA3/ESR (CLE)-related protein TDIF, partial CSB-YP375515ZBF1
CSB-EP375515ZBF1
CSB-BP375515ZBF1
CSB-MP375515ZBF1
CSB-EP375515ZBF1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rat Rho guanine nucleotide exchange factor 37 (Arhgef37) CSB-YP375516RA
CSB-EP375516RA
CSB-BP375516RA
CSB-MP375516RA
CSB-EP375516RA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Danio rerio Fin bud initiation factor (fibin) CSB-YP375517DIL
CSB-EP375517DIL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neisseria meningitidis serogroup A / serotype 4A NADH-quinone oxidoreductase subunit B (nuoB) CSB-YP375518NGF
CSB-EP375518NGF
CSB-BP375518NGF
CSB-MP375518NGF
CSB-EP375518NGF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neisseria meningitidis serogroup A / serotype 4A Probable tRNA threonylcarbamoyladenosine biosynthesis protein Gcp (gcp) CSB-YP375519NGF
CSB-EP375519NGF
CSB-BP375519NGF
CSB-MP375519NGF
CSB-EP375519NGF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neisseria meningitidis serogroup A / serotype 4A UPF0434 protein NMA0874 (NMA0874) CSB-YP375520NGF
CSB-EP375520NGF
CSB-BP375520NGF
CSB-MP375520NGF
CSB-EP375520NGF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neisseria meningitidis serogroup A / serotype 4A Polyribonucleotide nucleotidyltransferase (pnp), partial CSB-YP375521NGF
CSB-EP375521NGF
CSB-BP375521NGF
CSB-MP375521NGF
CSB-EP375521NGF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neisseria meningitidis serogroup A / serotype 4A tRNA 2-thiocytidine biosynthesis protein TtcA (ttcA) CSB-YP375522NGF
CSB-EP375522NGF
CSB-BP375522NGF
CSB-MP375522NGF
CSB-EP375522NGF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neisseria meningitidis serogroup A / serotype 4A Putative 8-amino-7-oxononanoate synthase (bioF) CSB-YP375523NGF
CSB-EP375523NGF
CSB-BP375523NGF
CSB-MP375523NGF
CSB-EP375523NGF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neisseria meningitidis serogroup A / serotype 4A UPF0756 membrane protein NMA2160 (NMA2160), partial CSB-YP375524NGF1
CSB-EP375524NGF1
CSB-BP375524NGF1
CSB-MP375524NGF1
CSB-EP375524NGF1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia enterocolitica serotype O:8 / biotype 1B Protein viaA (viaA) CSB-YP375525YAK
CSB-EP375525YAK
CSB-BP375525YAK
CSB-MP375525YAK
CSB-EP375525YAK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia enterocolitica serotype O:8 / biotype 1B Formamidopyrimidine-DNA glycosylase (mutM) CSB-YP375526YAK
CSB-EP375526YAK
CSB-BP375526YAK
CSB-MP375526YAK
CSB-EP375526YAK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia enterocolitica serotype O:8 / biotype 1B Der GTPase-activating protein YihI (yihI) CSB-YP375527YAK
CSB-EP375527YAK
CSB-BP375527YAK
CSB-MP375527YAK
CSB-EP375527YAK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia enterocolitica serotype O:8 / biotype 1B Orotate phosphoribosyltransferase (pyrE) CSB-YP375528YAK
CSB-EP375528YAK
CSB-BP375528YAK
CSB-MP375528YAK
CSB-EP375528YAK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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