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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Neisseria meningitidis serogroup C / serotype 2a UPF0133 protein NMC1380 (NMC1380) CSB-YP375909NEX
CSB-EP375909NEX
CSB-BP375909NEX
CSB-MP375909NEX
CSB-EP375909NEX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neisseria meningitidis serogroup C / serotype 2a 3-dehydroquinate dehydratase (aroD) CSB-YP375910NEX
CSB-EP375910NEX
CSB-BP375910NEX
CSB-MP375910NEX
CSB-EP375910NEX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neisseria meningitidis serogroup C / serotype 2a Ribonuclease PH (rph) CSB-YP375911NEX
CSB-EP375911NEX
CSB-BP375911NEX
CSB-MP375911NEX
CSB-EP375911NEX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neisseria meningitidis serogroup C / serotype 2a SsrA-binding protein (smpB) CSB-YP375912NEX
CSB-EP375912NEX
CSB-BP375912NEX
CSB-MP375912NEX
CSB-EP375912NEX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neisseria meningitidis serogroup C / serotype 2a tRNA-specific 2-thiouridylase mnmA (mnmA) CSB-YP375913NEX
CSB-EP375913NEX
CSB-BP375913NEX
CSB-MP375913NEX
CSB-EP375913NEX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neisseria meningitidis serogroup C / serotype 2a Histidinol-phosphate aminotransferase (hisC) CSB-YP375914NEX
CSB-EP375914NEX
CSB-BP375914NEX
CSB-MP375914NEX
CSB-EP375914NEX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neisseria meningitidis serogroup C / serotype 2a 2,3-bisphosphoglycerate-dependent phosphoglycerate mutase (gpmA) CSB-YP375915NEX
CSB-EP375915NEX
CSB-BP375915NEX
CSB-MP375915NEX
CSB-EP375915NEX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neisseria meningitidis serogroup C / serotype 2a Ribosomal RNA small subunit methyltransferase J (rsmJ) CSB-YP375916NEX
CSB-EP375916NEX
CSB-BP375916NEX
CSB-MP375916NEX
CSB-EP375916NEX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neisseria meningitidis serogroup C / serotype 2a Glutathione peroxidase homolog (gpxA) CSB-YP375917NEX
CSB-EP375917NEX
CSB-BP375917NEX
CSB-MP375917NEX
CSB-EP375917NEX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neisseria meningitidis serogroup C / serotype 2a Disulfide bond formation protein B (dsbB), partial CSB-YP375918NEX1
CSB-EP375918NEX1
CSB-BP375918NEX1
CSB-MP375918NEX1
CSB-EP375918NEX1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neisseria meningitidis serogroup C / serotype 2a Chorismate synthase (aroC) CSB-YP375919NEX
CSB-EP375919NEX
CSB-BP375919NEX
CSB-MP375919NEX
CSB-EP375919NEX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neisseria meningitidis serogroup C / serotype 2a Serine--tRNA ligase (serS) CSB-YP375920NEX
CSB-EP375920NEX
CSB-BP375920NEX
CSB-MP375920NEX
CSB-EP375920NEX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neisseria meningitidis serogroup C / serotype 2a Holo-[acyl-carrier-protein] synthase (acpS) CSB-YP375921NEX
CSB-EP375921NEX
CSB-BP375921NEX
CSB-MP375921NEX
CSB-EP375921NEX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neisseria meningitidis serogroup C / serotype 2a UDP-N-acetylmuramoylalanine--D-glutamate ligase (murD) CSB-YP375922NEX
CSB-EP375922NEX
CSB-BP375922NEX
CSB-MP375922NEX
CSB-EP375922NEX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neisseria meningitidis serogroup C / serotype 2a Shikimate dehydrogenase (aroE) CSB-YP375923NEX
CSB-EP375923NEX
CSB-BP375923NEX
CSB-MP375923NEX
CSB-EP375923NEX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neisseria meningitidis serogroup C / serotype 2a D-tyrosyl-tRNA (Tyr) deacylase CSB-YP375924NEX
CSB-EP375924NEX
CSB-BP375924NEX
CSB-MP375924NEX
CSB-EP375924NEX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neisseria meningitidis serogroup C / serotype 2a 50S ribosomal protein L21 (rplU) CSB-YP375925NEX
CSB-EP375925NEX
CSB-BP375925NEX
CSB-MP375925NEX
CSB-EP375925NEX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neisseria meningitidis serogroup C / serotype 2a NADPH-dependent 7-cyano-7-deazaguanine reductase (queF) CSB-YP375926NEX
CSB-EP375926NEX
CSB-BP375926NEX
CSB-MP375926NEX
CSB-EP375926NEX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neisseria meningitidis serogroup C / serotype 2a 30S ribosomal protein S21 (rpsU) CSB-YP375927NEX
CSB-EP375927NEX
CSB-BP375927NEX
CSB-MP375927NEX
CSB-EP375927NEX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neisseria meningitidis serogroup C / serotype 2a S-ribosylhomocysteine lyase (luxS) CSB-YP375928NEX
CSB-EP375928NEX
CSB-BP375928NEX
CSB-MP375928NEX
CSB-EP375928NEX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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